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CalGonJim 6/9 MONDAY 12am. Looks good. Using distilled water in a spray bottle for now, did a light mix of Grow Con .5ml per L ONE TIME...good. 6/10 weed good. In a kit stickers temperature indicators too hot icon shows hot face or something. without power like fishtanks sticker thermometer. 3pm BPP Picture today Analog special effects lost a day to lighting and special effects all practical im also a tiny bit demonic it adds to the character. 4pm look up fluid dispersal in root medium 6/13 5am everyone looks good medium lights. Now to try the fiberglass cubes.... tired of bugs hope I dont regret this... 6/14 1230pm looking good. no problems. very tired. found a little pair of unicorn slippers from a long time ago. probably headed to another Leaving Las Vegas melt down so who knows. 1pm 6/15 245am BPP is hard to grow. so its good for a small container grow since it seems to be a smaller plant, bigger containers dont make that much of a difference. It's this one is growing great setting 5 on AC Humidity dome now, with blue & red as well full power. Going to try fiberglass for all this time. 1053am IVE SEEN THINGS YOU PEOPLE WOULDN'T BELIEVE ....BECAUSE I CANT UPLOAD MY PRECIOUS PHOTOS... I CANT SPELL BELIVE...GOT DAMNITO

CalGonJim 6/16 12AM MONDAY looking good minimal nutrients. 0.5ml per L con. grow. 6/16 1145AM I am going to veg at higher than past use wattage. The main stalks aren't getting as big as they should. 6/17 320pm looking good they are in with some clone experiments. 6/18 11 am...SPIDER FARMER!!!! I JUST ORDERED THE SMALL WHATEVER THAT IS, DONT KNOW, IT LOOKS SO AWESOME!!!! I found an old folder when I went to cannabis University in Los Angeles must’ve been 2010⏰ 6/19 Its here VIVOSUN!!!! brand planters, AC INFINITY!!!! drying rack small perfect size 6 VIVOSUN trimmers & Ph Meters. 🚨🚨🚨SPIDERFARMER. ZERO STARS MOTHERFUCKERS!!!!! YOU ARE A LIGHT COMPANY AND YOU SELL A SYSTEM WITH NO DIMMER, NO HIGH, MEDIUM, LOW!!!!!!??????? 🚨🚨🚨 YOU ARE DONE. I WILL RETURN THIS AS SOON AS POSSIBLE, I WILL THROW THIS AWAY, AND EVERY SINGLE SPIDER FAMER TENT AND REPLACE IT WITH SOMEONE ELSE. 4PM I AM RETURNING IT, GODDAMN YOU IDIOTS...NO DONT PUT A DIMMER, OR HIGH MEDUM LOW SETTINGS ON A SMALL PLANTER WITH NO VENTILATION. MY FIRST LIGHTS LOOKED LIKE COUNTERFEIT leds, now this. NOW I HAVE TO DIG OUT THE FUCKING BOX AND PACK IT BACK UP.... LOSERS!!!! 430PM PACKED THAT POS BACK UP, NOW WAIT FOR AMAZON. WILL TRY A BETTER BRAND FOR MOBILE GERMINATION IDEAS. CUT OFF ALL THE LABLES ON THE SF TENTS, NEVER WANT TO BE REMINDED OF THAT SHIT COMPANY AGAIN. .. NOT THE FIRST TIME, THE LIGHTS HAS PROBLEMS TOO JUST HATE THIS COMPNAY NOW.E AC INFINITY, MARS HYDRO AND VIVOSUN DONT LIE BY OMMISON FOR A SALE OR SUFFER THE SAME FATE...LOOKING AT YOU VIVOSUN......... YOU'RE THE ADMIRALS NOW!!! SPIDER FARMER HAS BEEN FORCE CHOKED TO DEATH!! 6/219am. Setup a waterboarding and defoliation interrogation chamber for SERIOUS HST!!! THERE ARE 5 LIGHTS

CalGonJim 6/23 12am MONDAY. a gang of Mexicans have taken over my neighborhood. every thing from lawyers to landscapers all totally corrupt and running various scams, since they are cartel cops ill end up being setup or killed in the coming weeks. 110am. BIG DAY.... ROCKWOOL.... I am going to try this because I dont want to deal with dirt all the time. My experiments seem to fail the 1st few times...or always BUT THIS TIME I HAVE A GOOD FEELING ABOUT THIS! I firmly believe READING, WHICH IS ALWAYS BULLSHIT, or using AI, WHICH IS DEMONIC I WONT GO NEAR IT.... anything other than trial and error is like CHEATING, NO CHEAT CODES, THE MORE YOU BURN THE MORE YOU LEARN!!!!! Thinking of a Baderunner 2049 ending.. Wallace water lair Rachael clone....her eyes were green.....BANG....I chop the plant at that scene then sing off key Titanic song for no reason. im glad I lived in a time when you could bang a chick in the back of an old car and then sink a ship to get away from her. 6/24 215am I need a bandolier full of Churu treats, and a plastic army helmet....with more Churus on it & BORN TO CAT on the side...dry shave with the Churu's...protecting neighborhood cats from Invisible force field Predator..... I got all kinds of stupid ideas... 4am Star Trek Wrath of Dracula Khan. In Transylvania 200 years ago I was. a prince with power over 6 MILLIONS AH AH AH AH of people!!! An impossible number? How dare you???, IM THE VICTIM!!! 6/26 245am 6/27 11am Methylene Blue EXPERIMENT!!!!! anti-fungal 2 DROPS per liter mixed with nutrients.....GOING TO GO FUCK AROUND RIGHT NOOW!!!!!! All right I put some of my organic plugs into the rockwool PH 6.0 Ish 3 mL per liter, advanced nutrients grow half a milliliter of rapid start and two drops of methylene blue🤪👍👍👍🚨🚨 I used the generic. I did not want to use the expensive. Alex Jones brand for this experiment. I’m afraid I would contaminate it. 6//28 340am Methylene Blue EXPERIMENT!!!!! THEY ARE NOT DEAD YET... big win!!. 1PM THEY FINALLY UNDERSTAND THAT THE USA IS HAS BEEN TAKEN OVER BY GLOBALISTS, IT WORKS!!!!! 6/29 2 pm I found a Rolling Stone magazine w Hunter Thompson’s picture in it so I put that up on the wall. I use the slide gels whatever you call them the acetate rolled them up into cones with rubber bands and put them over. The lights. looks really good in here.👍👍👍👍

CalGonJim 6/31 145am MONDAY I waited too long to put them in vita-wool, they will be stuned but still good. I accidentally have 2 more plants than I expected so I have a RQS Gelato 44, it was supposed to be an Auto, but I got a free upgrade and was sent regulars and HOLDY Scti! THEY ARE MASSIVE....ITS THE Mike Tyson 2.0 seeds. 7/1 Experiment with this on other grow. This is a FAIL, I WAITED SO SO DAMN LONG I RUINED THE PLANTS....I will just plant new seeds and throw away SC, NOT WORTH KEEPING ALIVE........DOING IT ALL OVER AGAIN......STARTING......NOW!!!! 7/2 I used full strength 4ml Con Grow AND Mythelyne Blue AND Quick Sorb. Amino Acid FROM BIOTICS RESEARCH.... LIKE A MIRACLE DRUG ON ME SO I USED ON PLANTS HAS BEEN GOOD. Still 7/2 1150pm. ANOTHER watering 4ml Per L Con Grow ONLY this time. Taking up water fast, looking great. the lights might be a little too bright at 59watts these new lights I can veg at lower watts and flower with 93watts 7/3 145am. TOTAL LOSS. THIS GROW IS A TOTAL LOSS. GOT SO BUSY WITH PHOTOPERIODS IN THE OTHER GROW I F'D ALL OF THEM INTO STUNTED NOTHINGS.....FUCK. ALSO THE WOOL CUBES TAKE UP TOO MUCH SPACE....WHEN I TEAR OUT THE 4 RUNTS IM GOING TO SEE I I CAN GET THE WOOL TO FIT INSIDE A GALLON CONTAINER. I WILL CONSIDER WOOL A TOTAL FAIL FOR THIS MICRO-GROW STYLE..... THE WOOL CUBES ARE TOO BIG FOR SMALL SPACES REQUIRING A 10INCH ROUND SAUCER, INSTEAD OF 8 OR 6, MAYBE IF IT WERE IN A REGULAR SIZE CONTAINER BUT IMPRACTICAL AS HELL FOR ME. 7/3 5am🚨🚨👍⚡️⚡️FROM GROK I ASKED ABOUT QUICK SORB....What effects would Amino Acid Quick Sorb by Biotics Research have on plant growth??........There is no direct evidence or studies specifically examining the effects of Amino Acid Quick-Sorb by Biotics Research on plant growth. This product is designed as a human dietary supplement, containing nine free-form L-amino acids (Glycine, L-Alanine, L-Arginine HCl, L-Lysine HCl, L-Proline, L-Histidine HCl, L-Serine, L-Threonine, L-Valine) intended to support healthy blood glucose levels and other physiological functions in humans. However, based on general knowledge about amino acids and their role in plant physiology, we can infer potential effects if this product were applied to plants, with the caveat that it is not formulated or intended for agricultural use. Amino acids, when used as biostimulants in agriculture, can positively influence plant growth by enhancing nutrient uptake, improving stress resistance, and supporting metabolic processes. Research on amino acid-based biostimulants (not specific to Amino Acid Quick-Sorb) indicates they can improve photosynthesis, promote root development, increase nutrient assimilation, and enhance tolerance to abiotic stresses like drought, salinity, or extreme temperatures. For example, glycine and glutamic acid are involved in chlorophyll synthesis, which supports photosynthesis, while proline and arginine can enhance stress tolerance by stabilizing cell membranes and boosting antioxidant activity. If Amino Acid Quick-Sorb were applied to plants (e.g., via foliar spray or soil application), its amino acid content could theoretically provide similar benefits, as it contains several amino acids known to support plant physiology: * Glycine: Supports chlorophyll synthesis and nitrogen metabolism, potentially enhancing photosynthesis and growth. * L-Alanine: Involved in energy production and nitrogen assimilation, which could promote vegetative growth. * L-Arginine: Can enhance root development and stress tolerance by supporting hormone production. * L-Lysine: May contribute to stress tolerance through metabolic pathways, though it’s less abundant in plants naturally. * L-Proline: Known for improving stress resistance (e.g., drought, salinity) by stabilizing cell membranes and acting as an osmoprotectant. * L-Histidine: Supports enzyme activity and stress responses. * L-Serine: Contributes to protein synthesis and cell membrane formation, potentially aiding growth. * L-Threonine: Supports immune function and protein synthesis, which could enhance overall plant vigor. * L-Valine: A branched-chain amino acid that may support energy provision and growth under stress. However, several factors would influence the efficacy of Amino Acid Quick-Sorb on plants: 1. Formulation: The product contains additional ingredients like glycerol, citric acid, potassium sorbate, and sodium benzoate, which are not typically used in plant biostimulants. These could have unknown or potentially adverse effects on plants, such as altering soil pH or microbial activity. 2. Concentration and Application: The recommended human dosage (5 drops, ~0.16 ml, 1–3 times daily) is likely too low for effective plant application. Agricultural biostimulants typically use higher concentrations (e.g., 2 mL/L for products like Terra Sorb radicular). Improper dosing could lead to ineffective results or phytotoxicity. 3. Absorption: The sublingual delivery method for humans may not translate well to plants, which absorb nutrients primarily through roots or leaves. The product’s liquid form could be adapted for foliar or soil application, but its bioavailability to plants is untested. 4. Plant Species and Conditions: Effects vary by plant species and environmental conditions. For example, amino acids are more effective under stress conditions (e.g., drought, nutrient deficiency) than in optimal settings. Potential Effects on Plant Growth: * Enhanced Growth: The amino acids could stimulate protein synthesis, hormone production, and photosynthesis, leading to improved root, stem, and leaf growth. * Stress Resistance: Proline, arginine, and glycine may help plants cope with abiotic stresses, potentially improving resilience in challenging environments. * Nutrient Uptake: Amino acids can chelate micronutrients, improving their absorption and potentially boosting nutrient use efficiency. * Yield and Quality: If applied correctly, amino acids might increase yield components (e.g., fruit size, number) and improve quality (e.g., flavor, aroma). Limitations and Risks: * Lack of Specificity: Amino Acid Quick-Sorb is not designed for plants, and its amino acid profile may not be optimized for agricultural use compared to products like Terra Sorb or ATA NRG Alga-C. * Potential Toxicity: Non-amino acid components (e.g., preservatives) could harm plants or soil microbes if applied in high concentrations. * Cost and Practicality: Using a human supplement for plants is likely cost-prohibitive compared to dedicated agricultural biostimulants. Recommendation: While Amino Acid Quick-Sorb contains amino acids that could theoretically benefit plant growth, it is not formulated for this purpose, and no studies confirm its efficacy or safety in plants. For plant growth, consider using established amino acid-based biostimulants like Terra Sorb radicular, Terramin Pro, or ATA NRG Alga-C, which are designed and tested for agricultural use. If you wish to experiment with Amino Acid Quick-Sorb, start with a diluted solution (e.g., 1–2 drops per liter of water) applied as a foliar spray or soil drench, and test on a small number of plants to monitor for adverse effects. Consult with an agricultural expert to optimize application.🚨⚡️ I AM USING IT A FEW DROPS per L IN 2 LITERS OF NUTRIENT WATER PH'D TO 5.8ISH THE SYSTEM I AM INVENTING IS A HIGH PLANT STRESS ENVIROMENT, WITH TOO SMALL POTS IN TOO SMALL SPACE, BUT THAT IS THE GOAL OF A MICRO-GROW...MAKING IT WORK LIKE LANDING A ROCKET BACK AT THE PAD, IM GOING TO DO THAT WITH WEED. 🚨🚨 I had to consume several gallons of ice cream to find a container for the wool rock the wool cubes to sit in and keep them separate from the other grows and maybe do some more autos here very disappointed in this, but the amino acid and the methylene blue has had incredible effects on repairing these plants cause they are under super high stress🚨🚨👍👍👍 7/3 10am STARTED A NEW MPA (mystery seed) BY FASTBUDS AND A NEW NORTHERN LIGHTS!! EVERY TIME FASTBUDS HAS A BOGO I GET A 10 PACK OF MIX PACK AUTO SEEDS, THE BEST MOST COST EFFECTIVE WAY TO COLLECT AND EVERY SINGLE ONE IS A WINNER, NO MATTER WHAT!!! ONE OF THE VERY FEW COMPANIES THAT THE MARKETING AND THE GRAPHICS AND THE ARTISTIC VISION ALL MATCH THE PRODUCTS. VERY NICE. 7/4 5am They are repairing.....I cant believe it, amino acid and mblue with .25ml per L rhino skin, 4ml per l AN Con Bloom 7/5 1025am. Pinched the top off of NL today, she might get fully repaired the style Im using seems to do wonders!!! Holy Amino Acid Batman!!!! My plants piss blue too!!!! 🚨NEXT TRICK... NOW TO FIND EACH AMINO AND FIND IF IT CAN BE APPLIED AT DIFFERENT TIMES, DIFFERENT AMINOS . CAN THEY BE THROTTLED BY AMOUNT OR CO-APPLICATIONS WITH ALL THE OTHER?? NOTICE RESULTS BY EXTRA NITROGEN WITH SLIGHTLY BURNED TIPS EXPECTED!!! WOOOO! kyowa usa is one main mfgr. OH NO. I CAN BUY EACH OF THE AMINOS IN BULK... IM GOING ALL SPORES MOLD AND FUNGUS AGAIN... 245pm looking pretty good. Amino/ M Blue and 4ml L Con Grow pH5.8!!Doing HIGH doses stress testing. 7/6 3am NEW AMINO UNLOCKED 🚨 AI Overview Studies show that L-citrulline malate can have several positive effects on plants, particularly in relation to: 1. Stress Tolerance: Drought tolerance: Citrulline accumulates in plants like watermelon under drought conditions and acts as a potent hydroxyl radical scavenger, protecting leaves from oxidative stress. Salt tolerance: Citrulline accumulation can also be induced by salt stress, and the ratio of citrulline to proline may indicate salt tolerance in melon genotypes. Antioxidant defense: Exogenous application of citrulline can boost the activity of antioxidant enzymes like superoxide dismutase and catalase in plants like hyssop, particularly under water stress. 2. Growth and Development: Growth promotion: Foliar application of exogenous citrulline has shown positive effects on the growth rates and biomass accumulation of tomato and lettuce seedlings. Essential oil content: Citrulline application has been shown to increase essential oil content in hyssop, even under severe water stress. 3. Nitrogen Metabolism: Nitrogen translocation and transport: Citrulline appears to play a role in the translocation and long-distance transport of nitrogen within plants, being found in high concentrations in the phloem sap of cucurbits. Nitrogen storage: Citrulline is also implicated in nitrogen storage in plants. 4. Potential as a Biochemical Marker: Citrulline accumulation under stress conditions suggests it could be a useful biochemical indicator for screening and selecting plant varieties with enhanced drought and salt tolerance. Malate's Role: Malate, the other component of L-citrulline malate, is also crucial in plant nutrition. It's involved in nitrogen fixation, phosphorus acquisition, and aluminum tolerance. Overall, L-citrulline malate shows promising potential for use in agriculture to improve plant stress tolerance, growth, and nutrient utilization. Further research is needed to fully understand its effects and optimize its application in different plant species and growing conditions. 230PM WOW AFTER EXTREME NUTRIENTS AND HEAVY AMINO ACID COMPOUNDS FROM 2 PRODUCTS MEANT FOR HUMAN AND METHELYENE BLUE...... SPECTACULAR RESULTS. HEALING!!! NO BURNS LOOKING STRONG. VERY STRONG!!!! I also worked on my Basil.

CalGonJim 7/7 2am MONDAY. THE PLANTS ARE A TOTAL LOSS, BUT THE EXPERIMENT IS A TOTAL SUCCESS. I LEFT THEM IN GERMINATION TOO LONG AND ALL STUNTED. HOWEVER THE M. BLUE AND AMINO ACID WAS SUCCESSFUL. I CAN SEE THE PLANTS REPAIRING NOW. UNDER LESS HORRIBLE CONDITIONS THEY WILL FLOURISH. I AM GERMINATING 3 MORE TO REPLACE THESE. 1 Nitric Oxide pill per 2 L. 7 to 10 drops per 1 OR 2 L Quick Sorb. 1 drop per mL Methylene Blue 5 percent. and AN Con A+B 4ml per L, Rhino Silica .25ml per L. pH 5.7 - 6.2. 1235pm The plants in the this grow are fails. I have five clones as back ups a blueberry and five gorilla gas F1 from seedsman also sprouted a few others so we’ll see what happens.🌿👍👍 7/9 4pm tired. not liking the glass universe...I require life to make life not glass.... the aminos and mb dont work right in non life...I am training mitochondria and amino acids to jump and move and dance and juggle and complain about the you know whos ...I need soil biology for that. 7/10 230pm total loss, Critical Kush is outstanding she is indestructible!!!! I will start another and NOT forget her in germination for 2 months....tis time.....STARTING NOW..... tomorrow. paste from my other grow so I dont forget this SECRET I always say the quiet part LOUD!!!!......7/11 130AM MIKE TYSON 2.0 GOATS are still like nothing Ive seen!!!!!!!....I ALSO have G Gas F1 AND Bubbles Blueberry F4 clones as testers. I have poured FULL Strength everything and they are ROCK SOLID UNAFFECTED, UNBURNED!!!! 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨TOTAL SUCCESS!!!! NITRIC BOOST 1 pill per liter or 2 liter, 5 drops or more per L Quick Sorb by Biotics Research AMINOS, METHYLENE BLUE 5 percent 2 drops per L during veg, 1 drop during flower. .25ML (one quarter) PER L RHINO SILICA. Advanced Nutrients as a base. Bud Ignitor when appropriate pre flower time, then 1/2 strength BiG BUD at flower!!!! THAT IS THE SECRET FORMULA FOR GOD MODE!!!!! 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨 7/13 12 am.....This grow has become the relationship I thought I wanted because I was a desperate desperado saddy sad sad face dumb dumb....now I need a way to sabotage it and blame the plants and grow equipment and find a younger plant 🤔. 🚨⚡️💡⚡️🚨205am using a sheet of paper and some custom paper clips I found on Amazon cats of all things are the shape that work with these great rectangular Vivosun planters to make an umbrella for smaller plants to protect them from the light so this could be useful for germination in the tent as well I can have them under highlight but use my paper umbrella design🚨⚡️💡⚡️🚨👍

CalGonJim 7/14 MoNDAY. 3am. Time to cut the old ones and plant the new ones 7/14 1148PM STILL MONDAY!!! really at a loss here. Got more plants than I expected and I couldn't keep up and lost 4 plants because of it. I cant do so many plants even 2 extras really throws me off..... Might take some and reveg and start over. It's so damn hard to make a new diary.... I will kill these today and put some clones and see if I can change the seeds ....FOR SOME REASON I. CANT EDIT DIARY AFTER CREATION... 🚨 NO ENERGY SHOTS TODAY!!!! JUST COFFEE. ITS THE QUICK SORB AMINOS, NITRIC BOOST, METHELYENE BLUE, TMG POWDER, METHYL FOLATE PLUS, WOW!!! WHAT WORKS ON ME WORKS ON THE PLANTS, JUST ABSOLUTELY AMAZING!!!!!!!!!!! 7/16 Grow fail, found a way to take out the plants and not get bugs Critical Kush is the best but they all piss me off so I guess I cancel this and delete because you can NOT add or subtract seeds in the middle of a grow. great the entire thing is fucked. tearing out NL, and Straw. Ch., ...FUCK..FUCK FUCK......HAVE TO FUCKING TEAR THEM ALL FUCKING OUT NOW. 254am ...NO DONT PILLAGE ME....AND IM LIKE NO.....IM PILLAGING EVERYBODY, YOU INCLUDED!!!!!!!!!! HEY NORTHERN LIGHTS REMEMBER WHEN I SAID I'D KILL YOU LAST???? I LIED.. YOU ARE THE SULLY OF PLANTS, YOU'VE BEEN DROPPED. 6/21 I cant start a diary over so this one is just fucked

CalGonJim 7/21 630AM MONDAY we are having technical difficulties..... please stand by 7/24 11PM....... I HATE THIS WORLD.... DID YOU SEE WHAT GOD DID TO ME MAN!!!!????? I CANT GROW MORE THAN 6 PLANTS WITHOUT KILLING ALL OF THEM. I SWEAR I WASSNT ALWAYS THIS FUCKING RETARED,....OK I WAS OR AM GGGGGGOOOODDDDDAMMMMNIT!!! 4AM....I KILLED THE BANANA PURPLE PUNCH I STUCK IN THINKING NOBODY WOULD KNOW ...BUT THAT ONE DIED TOO.... CLEARLY GOD IS BEING A DICK TODAY. 7/25 830pm......update god is still being a dick. this was supposed to be done already. I tore out and threw away all the plants!!!! a BPP is just starting, bu ti have another grow with 3 but a Ricky didn't make it. iso im so pissed I have to start all the EXACT same seeds or im fucked ai cant fucking stand it when I lose an entire fucking grow like this. 7/26 I REALLY want Critiaal Kush and Straw...im going to start them over...then I will delete the parts where I screwed up THEN I will BLAME the seeds and viola dick...ITS NOT MY FAULT..... 7/27 1202am. copy paste 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨TOTAL SUCCESS!!!! NITRIC BOOST 1 pill per liter or 2 liter, 5 drops or more per L Quick Sorb by Biotics Research AMINOS, METHYLENE BLUE 5 percent 2 drops per L during veg, 1 drop during flower. .25ML (one quarter) PER L RHINO SILICA. Advanced Nutrients as a base. Bud Ignitor when appropriate pre flower time, then 1/2 strength BiG BUD at flower!!!! THAT IS THE SECRET FORMULA FOR GOD MODE!!!!! 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨 trying this on stupid yellow glass cubes

CalGonJim 7/28 12AM MONDAY......BPP...STARTING NOW!!!!!! SC, CK....PRETTY SOON....DID YOU SEE WHAT GOD DID TO ME????!!! 7/29 too much shit, cant merge diary,, plants were shit. deleting this grow and all others deleting this account soon. MIGHT START THIS GROW OVER LATER OR DELETE THIS ACCOUNT. A 7/31 130am adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know! 7/31 145am. Adding methylene blue (MB) to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, and L-glycine for cannabis plants introduces a compound with unique properties that may influence plant physiology, primarily through its roles as a redox agent, photosensitizer, and potential biostimulant. While methylene blue is more commonly studied in medical and aquatic contexts, its use in plant cultivation, including cannabis, is less documented, and specific studies on this exact combination are absent. Below, I’ll outline the potential effects of methylene blue on cannabis plants, its interactions with the other compounds, and the likely outcomes, drawing from available plant science knowledge and inferred mechanisms. Given the novelty of MB in this context, recommendations are cautious and speculative where direct evidence is lacking. ### Effects of Methylene Blue on Cannabis Plants Methylene blue (C16H18ClN3S) is a synthetic dye with redox, antimicrobial, and photosensitizing properties. Its effects on plants depend on concentration, application method, and environmental conditions: - **Antioxidant and Redox Activity**: At low concentrations (e.g., 0.1–10 µM or ~0.03–3 mg/L), MB acts as a redox mediator, scavenging reactive oxygen species (ROS) and protecting cells from oxidative stress caused by drought, high light, or salinity. In cannabis, this could enhance stress tolerance, particularly in high-intensity indoor grows or outdoor environments with temperature fluctuations. - **Photosynthesis Enhancement**: MB can act as an electron carrier in photosynthetic pathways, potentially improving electron transport in photosystem II. Studies on algae and some plants suggest low doses (e.g., 1–5 µM) may boost chlorophyll fluorescence and photosynthetic efficiency, potentially increasing cannabis biomass and bud quality. - **Antimicrobial Effects**: MB has antifungal and antibacterial properties, which could protect cannabis roots or foliage from pathogens like *Pythium* or *Botrytis* (bud rot). This is particularly relevant in hydroponic systems or humid environments. - **Stress Mitigation**: MB may stabilize cellular membranes under stress, similar to proline and TMG, potentially reducing damage from environmental extremes. - **Potential Toxicity at High Doses**: At higher concentrations (10–50 µM or ~3–15 mg/L), MB can generate ROS via photodynamic action under light, causing oxidative damage to plant tissues. This makes precise dosing critical. **Application Notes**: Two drops of MB (assuming a standard 1% solution, ~10 mg/mL, where 1 drop ≈ 0.05 mL) in, e.g., 1 liter of water yields ~1 mg/L (3 µM), a low concentration likely safe for foliar or root application. However, exact dosing depends on solution strength and volume. ### Synergistic Effects with EAAs, Proline, TMG, Glutamine, and L-Glycine Adding MB to the existing mix may enhance or modulate the effects on cannabis plants: 1. **Enhanced Stress Resilience**: - **MB + Proline/TMG/Glycine**: Proline, TMG, and glycine act as osmoprotectants and antioxidants, while MB scavenges ROS and stabilizes redox balance. Together, they could significantly improve cannabis tolerance to drought, heat, or salinity, reducing leaf wilting or nutrient stress in challenging environments (e.g., outdoor grows or high-EC hydroponics). - **Glutamine/EAAs Synergy**: Glutamine and EAAs support glutathione production, which complements MB’s antioxidant effects, potentially amplifying protection against oxidative stress from high light or nutrient imbalances. 2. **Growth and Biomass**: - **MB + EAAs/Glutamine/Glycine**: EAAs, glutamine, and glycine drive protein synthesis and nitrogen assimilation, while MB’s potential to enhance photosynthesis could provide the energy needed for these processes. This may lead to increased vegetative growth, larger leaves, and potentially denser buds in cannabis. - **Proline Contribution**: Proline supports tissue integrity, complementing MB’s membrane-stabilizing effects, which could enhance overall plant vigor. 3. **Secondary Metabolite Production**: - **MB + Glutamine/TMG/Glycine**: MB’s role as a photosensitizer may influence metabolic pathways by altering redox states, potentially enhancing synthesis of cannabinoids (e.g., THC, CBD) or terpenes. Glutamine and glycine contribute to precursor pathways (e.g., GABA, purines), while TMG’s methyl donation supports enzyme activity. This combination could theoretically increase terpene diversity or cannabinoid yield, though direct evidence is lacking. - **EAAs Role**: Methionine (an EAA) and glycine may support sulfur-based terpenes, with MB potentially amplifying metabolic efficiency via improved photosynthesis. 4. **Nutrient and Pathogen Management**: - **MB + Glycine/EAAs**: Glycine’s chelating properties and EAAs’ nutrient uptake enhancement could work with MB’s antimicrobial effects to improve nutrient availability and protect roots from pathogens in hydroponic or soil systems. - **TMG/Glutamine**: TMG’s cellular hydration and glutamine’s role in nitrogen metabolism may enhance MB’s ability to maintain cellular function under stress, improving overall nutrient efficiency. ### Potential Risks and Considerations - **Toxicity Risk**: MB at concentrations 10 µM (~3 mg/L) can produce ROS under light, damaging chloroplasts or cell membranes. Two drops in 1 liter (~1 mg/L) is likely safe, but overuse or concentrated solutions could harm cannabis, causing leaf burn or stunted growth. - **Absorption Competition**: MB may interact with amino acids during uptake, potentially reducing the efficacy of EAAs, glutamine, or glycine if applied simultaneously. Stagger applications (e.g., MB foliar spray, amino acids via root drench) to minimize this. - **Nitrogen Overload**: Adding MB to an already nitrogen-rich mix (EAAs, glutamine, glycine) increases the risk of toxicity, especially in cannabis, which is sensitive to excess nitrogen. Monitor for dark green leaves, clawing, or reduced flowering. - **Photodynamic Effects**: MB’s photosensitizing properties require careful application timing. Apply during low-light periods (e.g., early morning or evening) to avoid ROS generation in high-intensity grow lights. - **Limited Cannabis Data**: MB’s effects are mostly studied in algae, bacteria, or medical contexts, not cannabis. Its benefits are speculative, and plant-specific responses may vary by cultivar (e.g., sativa vs. indica). ### Practical Recommendations - **Dosing**: - MB: Two drops of 1% MB solution (~1 mg/L or 3 µM) per liter of water for foliar spray or root drench. Start with 1 drop/L to test tolerance. - EAAs: 150–300 mg/L - Glutamine: 250–500 mg/L - Proline: 50–100 mg/L - TMG: 0.5–1 g/L - Glycine: 100–500 mg/L - Apply twice weekly during vegetative growth or early flowering, adjusting based on plant response. - **Application**: - Foliar Spray: Apply MB and amino acids in low-light conditions to avoid phototoxicity. Use a fine mist to cover leaves evenly. - Root Drench: Suitable for hydroponics or soil, ensuring MB is diluted to avoid root damage. - Stagger Applications: Apply MB separately from amino acids (e.g., MB in the morning, amino acids in the evening) to reduce uptake competition. - **Monitoring**: Check for signs of stress relief (greener leaves, faster growth) or toxicity (yellowing, wilting). Test on a few plants first, especially for MB, as cannabis sensitivity is unknown. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. - **Cultivation Context**: - **Hydroponics**: Use lower MB doses (~0.5 mg/L) to avoid nutrient lockout or microbial disruption in sterile systems. - **Soil**: Ensure healthy soil microbiota, as MB’s antimicrobial effects could impact beneficial microbes if overused. - **Cultivars**: Sativa-dominant strains may benefit more from MB’s stress mitigation, while indicas may respond better to amino acid-driven growth. ### Evidence Gaps - **No Specific Studies**: No research addresses MB combined with EAAs, proline, TMG, glutamine, and glycine in cannabis. MB’s effects are extrapolated from algae, bacteria, or limited plant studies (e.g., Arabidopsis, lettuce). - **Cannabinoid/Terpene Impact**: MB’s influence on secondary metabolites is speculative. Its redox and photosynthetic effects may enhance terpene or cannabinoid synthesis, but cannabis-specific data are needed. - **Long-Term Effects**: MB’s long-term impact on cannabis growth, soil microbiota, or bud quality is unknown. Short-term studies suggest low doses are safe, but chronic use requires caution. - **X Search Results**: No recent posts on X discuss MB in cannabis cultivation with this combination. General posts mention MB for water treatment or medical uses, not plant applications. ### Conclusion Adding two drops of methylene blue (~1 mg/L) to a mix of EAAs, proline, TMG, glutamine, and glycine likely enhances cannabis stress tolerance, photosynthetic efficiency, and pathogen resistance, complementing the growth-promoting and nutrient-uptake benefits of the amino acids and TMG. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) effects on cannabinoid and terpene production. However, MB’s photodynamic and antimicrobial properties require precise dosing and application timing to avoid toxicity or microbial disruption. Start with low doses, test on a small scale, and monitor closely, consulting a cannabis cultivation expert for optimization. If you provide the MB solution concentration, application volume, or specific cannabis strain, I can refine dosing recommendations. Want me to search again for MB-related plant studies or focus on a specific aspect (e.g., MB’s photosynthetic effects)?"" 7/31 4am 🚨. Synergistic Effects with EAAs, Proline, TMG, Glutamine, L-Glycine, and Methylene Blue The NO capsule components may enhance the existing mix’s effects on cannabis plants: 1. Growth and Biomass: * Arginine/Citrulline + EAAs/Glutamine/Glycine: Arginine and citrulline boost NO production and nitrogen metabolism, complementing EAAs, glutamine, and glycine’s roles in protein synthesis and nutrient uptake. This could enhance cannabis vegetative growth, root development, and bud size. * Alpha-Ketoglutarate + Glutamine: Alpha-ketoglutarate supports the TCA cycle, amplifying glutamine’s role in nitrogen assimilation, potentially increasing photosynthetic efficiency and biomass. * Beta-Alanine: May support lipid metabolism, contributing to cell membrane formation and bud quality, though its low dose limits impact. 2. Stress Resilience: * Arginine/Citrulline + Proline/TMG/MB: Arginine and citrulline’s NO production enhances stress signaling, complementing proline and TMG’s osmoprotectant roles and MB’s antioxidant effects. Glycine’s glutathione production further bolsters stress tolerance. This mix could significantly improve cannabis resilience to drought, heat, or high light, reducing leaf wilting or nutrient stress. * Cannabis-Specific: Ideal for outdoor grows or high-intensity indoor setups where environmental stressors are common. 3. Secondary Metabolite Production: * Arginine/Citrulline + Glutamine/TMG/Glycine: NO from arginine and citrulline may upregulate pathways for cannabinoid and terpene synthesis, as NO influences phenylpropanoid and alkaloid pathways in plants. Glutamine, glycine, and TMG support precursor synthesis (e.g., GABA, purines, methylation), potentially enhancing THC, CBD, or terpene yields. * Beta-Alanine: Supports coenzyme A production, which may aid terpene synthesis, though its low dose may limit impact. * MB Synergy: MB’s photosynthetic enhancement could provide energy for NO-driven secondary metabolism, amplifying cannabinoid production, though this is speculative. 4. Nutrient Efficiency and Pathogen Resistance: * Arginine/Citrulline + Glycine/EAAs: Arginine and citrulline improve nitrogen use efficiency, complementing glycine’s chelating properties and EAAs’ nutrient uptake enhancement, optimizing cannabis growth in hydroponics or nutrient-poor soils. * MB Synergy: MB’s antimicrobial properties may synergize with NO’s role in plant defense signaling, protecting cannabis from pathogens like Botrytis or Pythium. Potential Risks and Considerations * Nitrogen Overload: The NO capsule adds arginine, citrulline, and beta-alanine, all nitrogen-containing compounds, to an already nitrogen-rich mix (EAAs, glutamine, glycine). This increases the risk of nitrogen toxicity in cannabis, causing dark green leaves, clawing, or reduced flowering. Dilute to 100–500 mg/L total and monitor plant response. * Absorption Competition: Arginine, citrulline, EAAs, glutamine, and glycine may compete for root or foliar uptake, reducing efficacy. Stagger applications (e.g., NO capsule components in the morning, others in the evening) or alternate days. * MB Interaction: MB’s redox activity may interact with NO from arginine/citrulline, potentially amplifying or disrupting signaling if doses are unbalanced. Keep MB at ~1 mg/L (e.g., two drops of 1% solution per liter). * Beta-Alanine Limited Impact: At 40 mg, beta-alanine’s effects may be negligible, as plant studies use higher concentrations (e.g., 100–500 mg/L) for measurable benefits. * Toxicity Risk: High doses of arginine or citrulline (1000 mg/L) may disrupt nitrogen balance, while MB (3 mg/L) could cause photodynamic damage. Test low doses first. * Cultivar Variability: Sativa-dominant cannabis may benefit more from stress mitigation (arginine, citrulline, proline, TMG, MB), while indicas may respond better to growth promotion (EAAs, glutamine, glycine). Practical Recommendations * Dosing: * NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * MB: ~1 mg/L (e.g., two drops of 1% solution per liter) * Apply twice weekly during vegetative growth or early flowering. * Application: * Foliar Spray: Apply during low-light periods (e.g., early morning) to avoid MB phototoxicity. Combine NO capsule components with amino acids for balanced uptake. * Root Drench: Suitable for hydroponics or soil, ensuring low MB concentrations to avoid microbial disruption. * Staggering: Apply NO capsule + MB separately from amino acids/TMG to minimize uptake competition (e.g., NO/MB in morning, others in evening). * Monitoring: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener leaves, robust growth). Test on a few plants first, especially for MB and NO components, due to limited cannabis data. * Synergy with Nutrients: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. * Cultivation Context: * Hydroponics: Use lower doses (~100 mg/L NO capsule, 0.5 mg/L MB) to avoid nutrient lockout or microbial imbalance. * Soil: Maintain healthy microbiota, as MB and high nitrogen inputs could disrupt beneficial microbes. * Cultivars: Adjust based on strain needs (e.g., stress-prone sativas vs. nutrient-hungry indicas). Evidence Gaps * No Specific Studies: No research addresses this exact combination (NO capsule + EAAs, proline, TMG, glutamine, glycine, MB) in cannabis. Effects are inferred from individual compound studies in plants (e.g., arginine for NO, MB in algae). * Cannabinoid/Terpene Impact: NO and MB may enhance secondary metabolism, but direct effects on THC/CBD or terpenes are unconfirmed. Cannabis-specific trials are needed. * Beta-Alanine Role: At 40 mg, beta-alanine’s impact is likely minimal, as plant studies use higher doses. * Long-Term Effects: Long-term impacts of MB and NO precursors on cannabis growth or soil health are unknown. Short-term use appears safe at low doses. Conclusion Adding a nitric oxide capsule (L-arginine, arginine AKG, L-citrulline, citrulline malate, beta-alanine) to EAAs, proline, TMG, glutamine, glycine, and MB likely enhances cannabis growth, stress tolerance, and nutrient efficiency by boosting NO signaling, nitrogen metabolism, and antioxidant defenses. The mix may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. However, nitrogen overload, absorption competition, and MB’s phototoxicity require careful dosing (e.g., 100–200 mg/L NO capsule, 1 mg/L MB). Test on a small scale, monitor closely, and consult a cannabis cultivation expert for optimization. If you provide the application volume (e.g., liters of water), specific strain, or growth stage, I can refine dosing further. Want me to search for studies on NO precursors in plants or focus on a specific aspect (e.g., NO’s role in cannabinoid synthesis)? 🚨. 7/31 STARTING OVER WITH STRAW. CHEESECAKE AUTO, CRITICAL +, AND APRICOT AUTO THIS TIME 8/1 1:25 AM apricot auto and strawberry cheesecake auto have sprouted I believe critical Kush is about to sprout. I think I see her coming up and I’ve decided to plant a cookie apple in a 2 gallon to see what the difference is between 1 gallon and 2 gallon size. 👍👍👍⚡️⚡️. Last time I started all of these I got caught up in another project and I didn’t transfer them out of the germination station for almost 5 weeks so they remain stunted and so I started them all over. That’s why they failed. The system is working, but I really fuck up and I lose a whole month and I don’t know where where it goes or what’s happening I’m kinda retarded.🤪👍👍. 245am L-GLYCINE AND Mg-Zyme by Biotics Research... LIKE NON DRUG TRANQ...KNOCKED ME OUT FOR 2 HOURS..WOW. VERY NICE!!!! 1130am all 3 look great, waiting for Cookie Apple in the 2 gallon to sprout. 8/1 1022PM Slept for 4 hours and feel great. We are all on amino acids now.!. 8/1 1057pm 🚨 Daily Consumption of a Specially Formulated Essential Amino Acid-Based Dietary Supplement Improves Physical Performance in Older Adults With Low Physical Functioning. 🚨 https://pmc.ncbi.nlm.nih.gov/articles/PMC8202157/ 🚨. 8/2 222am. 🚨⚡️🚨🚨⚡️⚡️. 🚨⚡️🚨🚨⚡️⚡️. AMINO FOLAR SPRAY SUCCESS!!!! THESE AMINOS DO NOT STINK SO SPRAYING IS NOT A PROBLEM. ADVANCED NUTES. GROW AT 1ML PER L WITH AMINOS GAVE A NICE FOLAR SPRAY.....NO BURNS, NO WILTING, JUST HAPPY HAPPY PLANTS. ACROSS CONES AND SEEDLINGS!!!!! ALSO WORKED ON NEW ROMAIN LETTUS, CHIVES, 4 VARIETIES OF NEW BASIL, EUCINASIA, AND ORANGE FLOWER SOMETHING!!!!!!🚨⚡️🚨🚨⚡️⚡️🚨⚡️🚨🚨⚡️⚡️. .

CalGonJim 8/4 1:36AM MONDAY. THIS IS MIXED WITH 2 OTHER GROWS...I HAVE RESEARCH NOTES IN 3 DIFFERNT PLACES SO... MAYBE 2:16 AM going to mix up the amino acids and go very light on advanced nutrients connoisseur because the amino‘s provide quite a bit of nitrogen and I don’t wanna overdo it. 💥. Monday 2:47 AM nutrient mix today: in a 2 L solution: one Nitric Boost capsule (nitric oxide (NO) capsule containing L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), and beta-alanine (40 mg), essential amino acids 300 mg, glutamine 500 mg, proline 100 mg, TMG powder 1 g, glycine 1 g, methylene blue 5% solution two drops, 3 mL each advanced nutrients connoisseur a and B in a 2 L solution solution pH balanced to 5.9. 💎🚨⚡️. Thank you for sharing the details of your nutrient mix for your cannabis grow. Your mix is quite complex, incorporating a variety of compounds, including nitric oxide (NO) precursors, amino acids, methylene blue, and Advanced Nutrients Connoisseur A and B, all in a 2 L solution pH-balanced to 5.9. Since you’re asking about this in the context of a cannabis grow and previously mentioned using essential amino acid powder and comparing FloraKleen vs. SledgeHammer, I’ll assume you’re seeking insight into how this nutrient mix might affect your cannabis plants, particularly in a hydroponic or soilless setup (given the pH of 5.9, which is typical for hydroponics or coco). I’ll evaluate the components, their potential effects on cannabis, interactions, and considerations for optimizing your grow, while addressing the inclusion of methylene blue and its relevance to plant health. ### Breakdown of Your Nutrient Mix Let’s analyze each component in your 2 L solution, its role in cannabis cultivation, and potential benefits or risks: #### 1. Nitric Boost Capsule - **Ingredients**: L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), beta-alanine (40 mg). - **Purpose in Humans**: These are nitric oxide (NO) precursors, used to increase NO production, which improves blood flow and muscle performance. In plants, NO plays a role in signaling, stress response, and growth regulation. - **Effects on Cannabis**: - **L-Arginine and L-Citrulline**: In plants, arginine is a key amino acid involved in nitrogen metabolism and polyamine synthesis, which supports cell division and stress resistance. Citrulline can contribute to arginine biosynthesis, potentially enhancing nitrogen availability. These may promote vegetative growth and root development, but excessive nitrogen from these compounds could delay flowering or reduce bud quality if not balanced. - **Arginine AKG**: AKG is a keto acid involved in the Krebs cycle, potentially boosting energy metabolism in plants. Limited research exists on its direct use in cannabis, but it may enhance nutrient uptake and photosynthesis. - **Beta-Alanine**: Less studied in plants, but it may act as a stress buffer, helping cannabis cope with environmental stressors (e.g., high light or temperature). Its low dose (40 mg) likely has minimal impact. - **Nitric Oxide Role**: NO in plants regulates stomatal closure, photosynthesis, and stress responses (e.g., drought, salinity). Low doses from these precursors could enhance resilience and growth, but high doses might disrupt hormonal balance (e.g., gibberellins, abscisic acid), potentially affecting flowering. - **Concentration**: Total 740 mg in 2 L = ~370 mg/L (0.37 g/L). This is a moderate dose, but the specific impact depends on how often you apply it and the growth stage. #### 2. Essential Amino Acids (300 mg) - **Purpose**: As discussed in your previous question, essential amino acids (e.g., lysine, methionine, threonine) reduce the energy plants spend on amino acid synthesis, supporting growth, terpene production, and stress resistance. - **Effects on Cannabis**: - Enhances protein synthesis, leading to stronger vegetative growth and potentially larger buds. - Increases terpene content (e.g., limonene, β-myrcene), improving aroma and flavor, but may reduce THCA (THC precursor) if overused, especially in late flowering. - Boosts nutrient uptake by chelating micronutrients, but can reduce calcium and iron levels, requiring monitoring. - **Concentration**: 300 mg in 2 L = 150 mg/L. This is a reasonable dose for hydroponics, aligning with grower recommendations (e.g., 1/8 tbsp per 12 gal ≈ 100–200 mg/L). #### 3. Glutamine (500 mg) - **Purpose**: Glutamine is a key amino acid for nitrogen transport and storage in plants, supporting chlorophyll synthesis and photosynthesis. - **Effects on Cannabis**: - Promotes lush vegetative growth and greener leaves, ideal for the vegetative phase. - Enhances stress tolerance by supporting glutathione production, which combats oxidative stress. - May contribute to nitrogen buildup, so use cautiously in late flowering to avoid affecting bud taste or potency. - **Concentration**: 500 mg in 2 L = 250 mg/L. This is a moderate-to-high dose, effective for vegetative growth but potentially excessive in late flowering. #### 4. Proline (100 mg) - **Purpose**: Proline is an amino acid that accumulates in plants under stress (e.g., drought, salinity), acting as an osmoprotectant and antioxidant. - **Effects on Cannabis**: - Improves stress resistance, particularly under high light, heat, or nutrient stress, common in indoor cannabis grows. - Supports pollen development and flowering, potentially increasing bud set. - Synergizes with other amino acids to enhance overall plant vigor. - **Concentration**: 100 mg in 2 L = 50 mg/L. This is a low, safe dose, effective for stress mitigation without risking nutrient imbalance. #### 5. TMG (Trimethylglycine/Betaine, 1 g) - **Purpose**: TMG is an osmoprotectant and methyl donor, used in plants to enhance stress tolerance and metabolic processes. - **Effects on Cannabis**: - Protects against environmental stress (e.g., high EC, temperature swings), improving plant resilience in hydroponic systems. - May enhance photosynthesis and nutrient uptake by stabilizing proteins and membranes. - Limited direct evidence for cannabis, but it’s used in agriculture to boost yield under stress. - **Concentration**: 1 g in 2 L = 500 mg/L. This is a high dose, potentially beneficial for stressed plants but unnecessary in optimal conditions, risking nutrient burn if overused. #### 6. Glycine (1 g) - **Purpose**: Glycine is an amino acid involved in chlorophyll synthesis, protein production, and chelation of micronutrients. - **Effects on Cannabis**: - Boosts photosynthesis, leading to greener, healthier plants. - Enhances nutrient uptake by chelating metals like zinc and iron, though your mix may already reduce iron/calcium, so monitor for deficiencies. - Supports terpene and cannabinoid synthesis indirectly via metabolic efficiency. - **Concentration**: 1 g in 2 L = 500 mg/L. This is a high dose, effective for vegetative growth but potentially excessive in flowering, as it may contribute to nitrogen buildup. #### 7. Methylene Blue (5% Solution, 2 Drops) - **Purpose**: Methylene blue is not a standard plant nutrient but has been explored in agriculture for its redox properties and potential antimicrobial effects. - **Effects on Cannabis**: - **Mitochondrial Enhancement**: In plants, methylene blue may act as an electron carrier, potentially improving mitochondrial efficiency and energy production, similar to its effects in humans (as you noted feeling great after taking it). This could enhance growth and stress tolerance. - **Antioxidant Properties**: At low doses, it may reduce oxidative stress, protecting cannabis from high light or nutrient stress. - **Antimicrobial Potential**: May suppress fungal or bacterial growth in the root zone, beneficial in hydroponics where pathogens can proliferate. - **Risks**: No established use in cannabis cultivation, and high doses could be phytotoxic or disrupt microbial balance in soil. The 5% solution (50 mg/mL) at 2 drops (~0.1 mL) = ~5 mg in 2 L = 2.5 mg/L. This is a very low dose, likely safe but with unproven benefits for plants. - **Note**: Its inclusion is experimental, and there’s limited research on methylene blue in cannabis. Your positive personal experience with it might suggest a hypothesis that it benefits plant metabolism similarly, but caution is warranted. #### 8. Advanced Nutrients Connoisseur A and B (3 mL each) - **Purpose**: Connoisseur A and B is a premium 2-part nutrient system designed for cannabis, providing macro- and micronutrients, with a focus on bloom enhancement. - **Effects on Cannabis**: - Supplies balanced NPK (nitrogen, phosphorus, potassium) and micronutrients (e.g., calcium, magnesium, iron) tailored for cannabis, promoting vigorous vegetative growth and heavy flowering. - Contains proprietary additives (e.g., humates, wetting agents) to enhance nutrient uptake and bud development. - High phosphorus and potassium in Part B support dense, resinous buds and terpene production. - **Concentration**: 3 mL each in 2 L = 1.5 mL/L (total 3 mL/L). This is within Advanced Nutrients’ recommended range (2–4 mL/L for bloom phase), suitable for flowering cannabis in hydroponics. #### 9. pH (5.9) - **Suitability**: A pH of 5.9 is ideal for hydroponic or coco cannabis grows, as it optimizes nutrient availability (most nutrients are bioavailable at pH 5.5–6.2). This suggests your setup is hydroponic or coco-based, aligning with the use of Advanced Nutrients and amino acids. ### Overall Effects on Cannabis - **Vegetative Phase**: - The mix is nitrogen-heavy (from L-arginine, glutamine, glycine, amino acids, and Connoisseur A), promoting lush vegetative growth, strong stems, and green leaves. Glutamine and glycine boost chlorophyll synthesis, while proline and TMG enhance stress resistance, ideal for early growth under high light or CO₂. - Nitric oxide precursors (L-arginine, L-citrulline) may enhance root development and nutrient uptake, supporting vigorous plants. - Methylene blue’s potential mitochondrial and antimicrobial effects could improve plant vigor, but its impact is speculative. - **Flowering Phase**: - Connoisseur A and B provide high phosphorus and potassium, essential for bud development and resin production. Amino acids (especially glycine, proline) may boost terpene content, enhancing aroma and flavor. - However, the high nitrogen content (from amino acids, glutamine, glycine, L-arginine) could delay flowering or reduce bud quality if used late in the cycle, as excess nitrogen can lead to leafy buds and lower THC. - Previous research (from your amino acid question) suggests amino acids may reduce THCA, so monitor potency if this is a priority. - Methylene blue’s role in flowering is unclear; it may help with stress but could disrupt late-stage metabolism if misapplied. - **Stress Resistance**: - Proline, TMG, and beta-alanine enhance resilience to environmental stressors (e.g., high EC, heat, light intensity), which is valuable in hydroponics. - Methylene blue’s antioxidant properties might complement this, but its efficacy is unproven. ### Potential Interactions and Risks 1. **Nitrogen Overload**: - The mix contains multiple nitrogen sources (L-arginine, glutamine, glycine, essential amino acids, Connoisseur A). This could lead to excessive vegetative growth, delaying flowering or causing nutrient burn (e.g., clawing leaves, dark green foliage). - **Solution**: Reduce amino acid doses (e.g., cut glutamine or glycine to 250 mg each) in late vegetative or early flowering to balance nitrogen. 2. **Nutrient Imbalances**: - Amino acids can reduce calcium and iron uptake (as noted in your previous question). Connoisseur A and B provide these micronutrients, but monitor for deficiencies (e.g., yellowing leaves, interveinal chlorosis). - **Solution**: Consider adding a calmag supplement (e.g., 1 mL/L CaliMagic) if signs of deficiency appear, especially in coco or hydroponics. 3. **Methylene Blue Risks**: - At 2.5 mg/L, methylene blue is unlikely to be phytotoxic, but its effects on cannabis are unstudied. It could disrupt beneficial microbes in the root zone or alter nutrient uptake if overused. - **Solution**: Use methylene blue sparingly (e.g., once weekly) and monitor plant response. Discontinue if you notice stunted growth or discoloration. 4. **High TMG and Glycine Doses**: - At 500 mg/L each, TMG and glycine are high compared to typical plant applications (100–300 mg/L). While beneficial for stress, they may contribute to nutrient buildup or EC spikes. - **Solution**: Reduce to 250–300 mg/L and test EC (aim for 1.2–2.0 mS/cm in bloom). 5. **EC and pH Stability**: - The mix’s complexity (multiple amino acids, NO precursors, nutrients) could raise EC, risking nutrient burn. The pH of 5.9 is good, but frequent monitoring is needed as amino acids and acids in Connoisseur can lower pH over time. - **Solution**: Measure EC (should be ~1.0–1.5 mS/cm in veg, 1.5–2.2 mS/cm in bloom) and adjust nutrient strength if needed. ### Recommendations for Optimization 1. **Adjust for Growth Stage**: - **Vegetative Phase**: Your mix is well-suited for vegetative growth due to high nitrogen and stress-protective compounds. Continue as is, but monitor EC to avoid overfeeding. - **Flowering Phase**: Reduce nitrogen-heavy components (glutamine, glycine, essential amino acids) to 50% (e.g., 150 mg amino acids, 250 mg glutamine/glycine) starting week 3 of flowering to focus on bud development and prevent nitrogen buildup. Increase Connoisseur A and B to 4 mL/L in mid-bloom for higher PK. - **Final Flush**: Use FloraKleen (recommended from your previous question) at 5–10 mL/gal for the first 2–3 days of a 10-day flush, followed by RO water, to clear excess nitrogen and salts from amino acids and NO precursors. Stop methylene blue during flush to avoid residues. 2. **Monitor Nutrient Levels**: - Check for calcium/iron deficiencies (e.g., yellowing, spotting) due to amino acid chelation. Add a calmag supplement if needed. - Use a TDS meter to keep EC in check (1.2–2.2 mS/cm depending on stage). 3. **Methylene Blue Caution**: - Given its experimental nature, limit methylene blue to 1–2 drops per 2 L weekly and observe plant response. If no clear benefits (e.g., faster growth, greener leaves) are seen after 2–3 weeks, consider discontinuing to simplify the mix. - If you’re including it based on your positive personal experience, its low dose is unlikely to harm plants, but benefits are speculative. 4. **Flushing Strategy**: - Given your complex mix, periodic flushing (every 3–4 weeks) with FloraKleen (5 mL/gal) is recommended to prevent salt buildup, especially in hydroponics. This aligns with your previous interest in FloraKleen vs. SledgeHammer, where FloraKleen was preferred for hydroponic cannabis. 5. **Terpene and Cannabinoid Focus**: - Your amino acid-heavy mix may boost terpenes (e.g., limonene, β-myrcene), enhancing bud aroma, but could reduce THCA. To maximize THC, reduce amino acids in weeks 5–8 of flowering and rely on Connoisseur B’s high PK. - Consider adding a bloom booster (e.g., Advanced Nutrients Bud Candy, 2 mL/L) to enhance terpenes and sugars without nitrogen overload. ### Addressing Methylene Blue Your inclusion of methylene blue is intriguing, likely inspired by its benefits for you (energy, mood) and its potential to enhance mitochondrial function or reduce oxidative stress. In plants, its redox properties might theoretically improve energy metabolism or protect against stress, but there’s no direct evidence for cannabis. The low dose (2.5 mg/L) minimizes risks, but I’d recommend treating it as an experiment: - **Test**: Apply to a single plant or reservoir for 2 weeks and compare growth, leaf color, and bud development to untreated plants. - **Discontinue if No Benefit**: If no improvement is seen, simplify your mix to avoid potential residues or interactions. ### Conclusion Your nutrient mix is robust for vegetative growth, with amino acids (glutamine, glycine, proline, essential amino acids), NO precursors, and Connoisseur A and B promoting vigorous plants and stress resistance. However, the high nitrogen content and experimental methylene blue require careful management to avoid delays in flowering or reduced THC potency. For optimal results: - Reduce nitrogen-heavy components (glutamine, glycine, amino acids) in flowering to focus on bud development. - Use FloraKleen for periodic and final flushes to clear excess salts, especially given the amino acid and NO precursor load. - Monitor EC, pH, and micronutrients (calcium, iron) to prevent imbalances. - Treat methylene blue as an experiment and discontinue if no clear benefits are observed. If you can share more details (e.g., growth stage, hydroponic vs. coco, strain type, or specific goals like THC vs. terpenes), I can refine the recommendations further. I can also search X or recent studies for grower experiences with similar mixes or provide a tailored nutrient schedule. Let me know how you’d like to proceed! 🚨. 8/4 6:13am!!! monday morning after feeding ....WHAT A FUCKING PAIN IN THE ASS TO MEASURE OUT THE AMINO POWDERS....NO WAY ANYONE WOULD DO THIS. THEY WILL HAVE TO BE EN ENCAPSULATED.... MAYBE HAVE THEM MADE?? WHAT GREAT RESULTS....WOW NO BURNS EVEN ON A JUST GERMINATED COOKIE APPLE... HER STEM IS ALREADY GLOWING!!!!! NO BURNS....AMINOS WORKING.....but still what a huge pain in the ass to measure powders!!! 8/4 9:02AM LOOKING GREAT, SUCCESS. AFTER 3 HOURS....NO STRESS NO BURNS EVEN ON SEEDLING COOKIE APPLE IN 2 GALLON THAT JUST SRPOUTED YESTERDAY. TRIPLOID OG AND OTHER COOKIE APPLE AND DR. SEEDSMAN CBD IN GLASS WOOL ARE SHOWING HUGE GROWTH SPIKES, FLORECENT COLOR CHANGES, GREEN BRIGHT PHOTOSYNTHESIS ENHANCED GREEN....IM SO FUCING OUT OF THIS WORLD AMAZING.....COULD BE THE ENERGY SHOT & COFFEE AND ALL THE SUPPLEMENTS....IDK. 8/4 11:50am MERGING DIARY.... WONT UPDATE OG TRIPLOID, USING THIS AS THE MAIN UNTIL HARVEST. 2 COOKIE APPLES, ALL AUTOFLOWERS, MUCH EASIER. EVERYONE LOOKS GREAT, HAPPY AND GREEN, NO STRESS...ALMOST LIKE THEY HAVE BEEN GIVEN A MAGIC POTION FROM AN OLD WIZARD THAT PROTECTS THEM FROM HOW STUPID THE WIZARD CAN BE. 🚨🚨8/4 12:30pm !!! FOR HUMANS!!!!!!🚨🚨👨‍🔬Combining **L-glutamine**, **proline**, **essential amino acids (EAAs)**, **glycine**, and **trimethylglycine (TMG)** in your supplement regimen, as seen in the nutrient mix you described for your cannabis grow, can have significant effects on human health, particularly given your positive experience with methylene blue (“I feel great after I take it”). Since you’re asking about the benefits for yourself (not plants), I’ll focus on how these compounds may contribute to your well-being, their synergistic effects, and how they might align with the energy and mood boost you’ve reported. I’ll also consider the context of your previous questions, including the nitric oxide (NO) precursors and methylene blue, to provide a comprehensive picture. The response will be concise yet thorough, addressing mechanisms, benefits, and potential considerations. ### Overview of Each Component and Their Benefits for Humans 1. **L-Glutamine (500 mg in your mix)**: - **Role**: A conditionally essential amino acid, critical for gut health, immune function, and muscle recovery. - **Benefits**: - **Gut Health**: Supports intestinal lining repair, reducing leaky gut and improving digestion, which can enhance overall well-being and energy. - **Immune Support**: Fuels immune cells (e.g., lymphocytes), boosting resilience, especially during stress or illness. - **Muscle Recovery**: Reduces muscle soreness and supports protein synthesis, beneficial if you exercise or are physically active. - **Brain Health**: Serves as a precursor to neurotransmitters like glutamate, potentially improving focus and mood. - **Relevance to You**: The energy and mood boost you feel may partly stem from glutamine’s role in reducing inflammation and supporting brain function. 2. **Proline (100 mg in your mix)**: - **Role**: A non-essential amino acid involved in collagen synthesis, tissue repair, and antioxidant defense. - **Benefits**: - **Joint and Skin Health**: Promotes collagen production, supporting joint flexibility, skin elasticity, and connective tissue repair. - **Cardiovascular Health**: Supports arterial wall integrity, potentially complementing the NO precursors in your mix (L-arginine, L-citrulline). - **Stress Resistance**: Acts as an osmoprotectant, helping cells manage oxidative stress, which may contribute to your “feeling great.” - **Relevance to You**: Proline’s low dose may subtly support tissue health and stress resilience, enhancing your overall vitality. 3. **Essential Amino Acids (EAAs, 300 mg in your mix)**: - **Role**: EAAs (e.g., leucine, lysine, methionine) are amino acids the body cannot synthesize, essential for protein synthesis and metabolic functions. - **Benefits**: - **Muscle Maintenance**: Stimulates muscle protein synthesis, aiding recovery and preventing muscle loss, especially during exercise or aging. - **Energy and Mood**: EAAs like leucine support energy metabolism, while tryptophan and phenylalanine are precursors to serotonin and dopamine, potentially boosting mood. - **Metabolic Health**: Supports insulin sensitivity and nutrient metabolism, contributing to sustained energy. - **Relevance to You**: EAAs may enhance your energy and mood by supporting muscle recovery and neurotransmitter production, aligning with your positive response to the mix. 4. **Glycine (1 g in your mix)**: - **Role**: A non-essential amino acid involved in collagen synthesis, neurotransmitter regulation, and detoxification. - **Benefits**: - **Sleep and Mood**: Acts as an inhibitory neurotransmitter, promoting relaxation and improving sleep quality, which can enhance mood and energy. - **Cognitive Health**: Supports NMDA receptor function, potentially improving memory and focus. - **Collagen and Joint Health**: Like proline, supports connective tissue and skin health. - **Detoxification**: Aids in glutathione production, reducing oxidative stress and supporting liver function. - **Relevance to You**: Glycine’s calming effects and antioxidant support may contribute significantly to your “feeling great,” especially if you experience better sleep or reduced stress. 5. **Trimethylglycine (TMG, 1 g in your mix)**: - **Role**: Also known as betaine, TMG is a methyl donor involved in homocysteine metabolism and cellular protection. - **Benefits**: - **Cardiovascular Health**: Lowers homocysteine levels, reducing heart disease risk, and may synergize with NO precursors for better blood flow. - **Exercise Performance**: Enhances hydration and muscle endurance, potentially improving physical energy. - **Liver Health**: Supports fat metabolism and detoxification, reducing liver stress. - **Mood and Stress**: Methylation supports neurotransmitter synthesis, potentially boosting mood. - **Relevance to You**: TMG’s role in energy metabolism and methylation may amplify the mood and energy benefits you’re experiencing. ### Synergistic Benefits of the Combination The combination of L-glutamine, proline, EAAs, glycine, and TMG, alongside the NO precursors (L-arginine, L-citrulline, etc.) and methylene blue from your mix, creates a synergistic effect that likely explains why you “feel great”: 1. **Enhanced Energy and Recovery**: - **Glutamine, EAAs, and TMG**: Support muscle protein synthesis, energy metabolism, and recovery, reducing fatigue and boosting physical vitality. Beta-alanine (from the NO capsule) buffers lactic acid, enhancing exercise endurance. - **NO Precursors**: L-arginine and L-citrulline increase NO production, improving blood flow and oxygen delivery to muscles and brain, enhancing energy and focus. - **Methylene Blue**: As you noted, methylene blue enhances mitochondrial efficiency, boosting ATP production, which complements the energy-supporting effects of glutamine, EAAs, and TMG. 2. **Mood and Cognitive Boost**: - **Glycine and EAAs**: Glycine promotes relaxation and sleep, while EAAs (e.g., tryptophan, phenylalanine) support serotonin and dopamine production, enhancing mood and mental clarity. - **TMG**: As a methyl donor, supports neurotransmitter synthesis and reduces homocysteine, which is linked to mood disorders. - **Methylene Blue**: Its mild MAO inhibition may increase serotonin and dopamine levels, amplifying the mood benefits you’re experiencing. 3. **Stress Reduction and Resilience**: - **Proline, Glutamine, and Glycine**: Act as antioxidants and osmoprotectants, reducing oxidative stress and supporting cellular health under physical or mental stress. - **TMG**: Enhances methylation, supporting stress resilience and detoxification. - **Methylene Blue**: Its antioxidant properties may reduce cellular stress, complementing the other compounds. 4. **Cardiovascular and Tissue Health**: - **Proline, Glycine, and TMG**: Support collagen synthesis and cardiovascular health, improving joint, skin, and arterial function. - **NO Precursors**: Enhance vasodilation, improving blood flow and nutrient delivery, which may contribute to your overall sense of well-being. ### Why You Feel Great Your positive response (“I feel great”) likely stems from the combined effects of: - **Mitochondrial and Energy Boost**: Methylene blue and glutamine enhance ATP production and energy metabolism, giving you a physical and mental lift. - **Neurotransmitter Support**: Glycine, EAAs, TMG, and methylene blue support serotonin, dopamine, and GABA activity, improving mood, focus, and relaxation. - **Reduced Stress**: Proline, glutamine, and TMG combat oxidative stress and support recovery, reducing fatigue and enhancing resilience. - **Improved Blood Flow**: NO precursors increase circulation, delivering more oxygen and nutrients, which may enhance energy and cognitive clarity. The doses in your mix (500 mg glutamine, 100 mg proline, 300 mg EAAs, 1 g glycine, 1 g TMG) are within typical supplemental ranges, suggesting a balanced approach that avoids overload while providing cumulative benefits. ### Considerations and Precautions 1. **Dosage Safety**: - Your doses are moderate and generally safe for most adults, but long-term high doses of TMG (1 g daily) or glutamine (500 mg daily) may stress the liver or kidneys in sensitive individuals. Typical supplemental doses are 500–3,000 mg for TMG and 5–10 g for glutamine, so you’re on the lower end. - Methylene blue (2 drops of 5% solution ≈ 5 mg) is a low dose, but its long-term use lacks extensive human studies. Monitor for side effects like nausea or dizziness. 2. **Interactions**: - **Methylene Blue and SSRIs**: Methylene blue is a mild MAO inhibitor and can cause serotonin syndrome if combined with SSRIs or other serotonergic drugs. Consult a doctor if you’re on such medications. - **NO Precursors**: L-arginine and L-citrulline may lower blood pressure, so monitor if you have hypotension or take related medications. - **TMG and Methylation**: High TMG doses may overstimulate methylation, potentially causing anxiety in some individuals. Your 1 g dose is moderate but worth monitoring. 3. **Individual Response**: - Your positive response suggests your body responds well to this mix, possibly due to baseline deficiencies (e.g., in glycine or glutamine) or high metabolic demands (e.g., from exercise or stress). Genetic factors affecting methylation (e.g., MTHFR mutations) may make TMG particularly effective for you. 4. **Nutrient Timing**: - Taking this mix in the morning (as implied by 2:47 AM) aligns with boosting energy and focus for the day. However, glycine’s calming effects might be better utilized at night for sleep support. Consider splitting doses (e.g., glycine in the evening, others in the morning). ### Recommendations - **Optimize Timing**: Take glutamine, EAAs, and TMG in the morning for energy and recovery, and glycine in the evening for relaxation and sleep. Proline can be taken anytime due to its structural role. - **Monitor Effects**: Track specific benefits (e.g., energy, mood, sleep, exercise recovery) to identify which compounds drive your “feeling great.” If methylene blue is key, its mitochondrial and mood effects may be central. - **Hydration and Diet**: Ensure adequate hydration and a balanced diet, as amino acids and TMG increase metabolic demands. Pair with B vitamins (especially B6, B12, folate) to support methylation and amino acid metabolism. - **Consult a Professional**: Discuss this regimen with a doctor or nutritionist, especially regarding methylene blue and NO precursors, to rule out interactions or contraindications (e.g., G6PD deficiency for methylene blue). - **Adjust Doses if Needed**: If you notice side effects (e.g., jitteriness from TMG, GI upset from glutamine), reduce doses (e.g., 500 mg TMG, 250 mg glutamine) and reassess. ### Conclusion The combination of L-glutamine, proline, EAAs, glycine, and TMG likely contributes to your feeling great by boosting energy (via mitochondrial support and muscle recovery), enhancing mood (through neurotransmitter production), reducing stress (via antioxidant and osmoprotectant effects), and supporting cardiovascular and tissue health. Methylene blue and NO precursors from your mix amplify these benefits by improving mitochondrial function and blood flow. Your moderate doses are generally safe, but careful monitoring and medical consultation are advised, especially for methylene blue. If you’d like, I can search for recent studies or X posts on these supplements for human use, or tailor a dosing schedule based on your goals (e.g., energy, mood, recovery). Let me know how you’d like to proceed!🚨👨‍🔬. 8/4 1230pm. 8/5 9 pm. They look pretty good, long day 12 hours cleaning the tents and plants and room, lots of fun. 8/6 11:21pm GOING AMINO....BUT....THE NITRIC OXIDE PILLS ARE ONLY GOOD FOR THE 1ST MONTH. EXTRA N UPTAKE IS GOOD FOR LEAFY GREENS BUT WILL HINDER FLOWER AND STALK STRENGTH & GROWTH WITH OVER N, AND SOFTENS STALKS EVEN WITH THE APPLICATION OF SILICA. MB MAYBE 1 DROP FOR 2L IT TURNES THE LEAF SURFACE BLUE-ISH AND SEEMS TO PROTECT A LITTLE. 8/7 5am doing amino‘s today full strength in 2 L solution one drop of methylene blue 5%. I’ll be doing half strength connoisseur grow.🌿💦💦💦👍👍. 6 AM all finished watering full dose amino’s half dose connoisseur grow hope this works out.👍👍👍 8/7 1:25 PM. It looks like the Inquisitors captured OG Triploid. It was really sad and they cut his head off and hung him from the lights☠️I blame Obi-Wan😡. Everyone else is looking great.😂👍👍👍. 😡😡😡

CalGonJim 8/11 1:21 AM Monday. everyone is doing great. I did very heavy amino‘s yesterday so far so good no problems yet.👍👍👍👍 11AM MONDAY.... .Aminos worked great!!!!!!! They are all growing better than expected and some info on the same mix I used on the plants on ME....... 8/11 10:45AM 🚨👨‍🔬 AMINO COMBO -LOW DOSE TEST - SUCCESS ON ME NOT THE PLANTS - HUMAN TRIAL, Combined effects of EAA, proline, l glutamine, TMG powder, l glycine.... AI Overview A combination of Essential Amino Acids (EAAs), Proline, L-Glutamine, TMG powder, and L-Glycine could potentially offer a range of synergistic benefits, impacting various aspects of health and physical performance. Let's explore some of these potential combined effects: 1. Muscle protein synthesis and recovery EAAs: Essential Amino Acids are crucial building blocks for protein synthesis, particularly important for muscle growth and repair, especially after exercise or in individuals with low protein intake. Proline: As a key component of collagen, proline supports the structural integrity of muscles and connective tissues, aiding in repair and strength. L-Glutamine: This abundant amino acid supports nitrogen balance and is involved in muscle tissue repair and recovery from strenuous activity. L-Glycine: Contributes to collagen formation and creatine production, both vital for muscle function, growth, and repair. In combination, these could enhance the body's ability to build and repair muscle tissue, leading to improved strength, reduced soreness, and faster recovery from exercise. 2. Gut and immune health L-Glutamine: Serves as a primary fuel source for intestinal cells and helps maintain the integrity of the gut lining, potentially protecting against conditions like leaky gut syndrome. Proline: As a collagen precursor, it also contributes to a strong intestinal lining. L-Glycine: Supports a healthy immune response and may help limit inflammation. Combined, these amino acids may work together to strengthen the gut barrier, reduce inflammation, and support a robust immune system. A healthy gut microbiome, influenced by amino acid intake, is further linked to a strong immune response. 3. Connective tissue and skin health Proline and L-Glycine: Both are essential for collagen synthesis, a protein critical for skin, bones, and connective tissues like tendons and ligaments. Proline supports skin elasticity and wound healing, while glycine contributes to skin structure and repair. Together, they could boost collagen production, leading to improved skin elasticity, reduced wrinkles, and faster healing of wounds and injuries. 4. Metabolic and cardiovascular health TMG powder: Supports healthy homocysteine levels, a key indicator of heart health, by converting it into methionine. L-Glycine: May help regulate blood sugar levels, improve insulin sensitivity, and has been associated with a lower risk of heart attacks. EAAs: Can influence metabolism, including glucose and lipid balance. The combined impact on homocysteine, blood sugar, insulin sensitivity, and antioxidant defenses suggests a beneficial influence on overall metabolic function and cardiovascular health. 5. Other potential benefits L-Glycine: May improve sleep quality by lowering body temperature and increasing serotonin production. It also has a calming effect on the brain, potentially reducing anxiety. EAAs: May contribute to improved mood and cognitive function. Important considerations Consult a healthcare professional: Before taking any supplements, it is crucial to consult with a healthcare provider, especially if you have pre-existing medical conditions, are pregnant or breastfeeding, or are taking medications. Dosage: Optimal dosages for these supplements are not always clearly defined and may vary depending on individual needs. Potential side effects: While generally considered safe, some individuals may experience mild side effects like gastrointestinal discomfort with glutamine or glycine supplementation. In summary, a combination of EAAs, Proline, L-Glutamine, TMG, and L-Glycine could synergistically support muscle building and repair, boost immune function, improve gut health, enhance skin and connective tissue integrity, and potentially contribute to improved metabolic and cardiovascular health. However, it's essential to approach supplementation thoughtfully, consulting with a healthcare professional to ensure safety and appropriateness for your individual needs. "" 🚨🚨. 8/12 1045PM. Cookie Apple is growing FAST, maybe 3 colas in a 1 gallon is the way to go with her?? CA 2 is in 2 gallon and doing good. Tonight will be FULL AMINOS for the 4 still in Veg WITH NITRIC OXIDE PILLS. BUT!!! No NO pills OR MB for the ones with flowers or transitioning.🚨. NO pills are only good for the first 2 weeks or 4 pairs of leaves or less. Once they start getting big and bushy IMMEDIATELY stop NO & MB and continue with PROLINE 100MG L, GLYCINE 500MG L, GLUTAMINE 500MG L, TMG POWDER 1g L, EAA 300MG L. AN CONN. 3ML L. 5.9PH (my city water will bounce the pH back up a few points so I have to make sure it stays under 6.5, too close to that and it goes over). 11pm on second thought not doing 2 separate mixes...too hard...so no NO pill and no MB! 8/13 3am looks great. Full Aminos then split 2L into: 1L with AN Con. Grow 2ml per L & 1L with AN Con Bloom 2.5ml per L. Did LST on 3 Cola Cookie Apple in 1 gallon. They have all been very strong and have responded well to their environment including lighting at 100W and only 8 inches about above canopy. 8/13 9am Application Guidelines • Nutrient Solution Recipe (2 L): ◦ Water: 2 L (RO or dechlorinated tap water). ◦ Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). ◦ Atami Bloombastic: 1 mL (0.5 mL/L, suitable for flowering autoflowers). ◦ Betaine Anhydrous: 250–500 mg (1–2 mM, start with 250 mg). ◦ Proline: 230–460 mg (1–2 mM, start with 230 mg). ◦ Mixing: Add Bloom first, then Bloombastic, betaine, and proline, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). • Timing: Apply during vegetative (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Stop both betaine and proline 2 weeks before harvest to allow flushing for optimal bud flavor. • Frequency: Use with each nutrient feeding (e.g., every 2–3 days in hydro, weekly in soil). Alternate or reduce frequency if EC approaches 1.8 mS/cm. • Source: Use L-proline (plant-grade, 99%+ purity) from a reputable supplier (e.g., agricultural or lab-grade sources). Avoid impure or non-plant-specific products. Potential Risks and Considerations • Nutrient Overload: Combining betaine (250–500 mg) and proline (230–460 mg) with Bloom and Bloombastic increases EC and nitrogen load (proline is an amino acid). Monitor for nutrient burn (brown leaf tips, clawing). If symptoms appear, reduce both to 0.5 mM (125 mg betaine, 115 mg proline per 2 L) or skip a feeding. • EC Impact: Betaine and proline each add ~0.1–0.2 mS/cm at 1–2 mM. With Bloom (1–2 mL/L) and Bloombastic (0.5 mL/L) at ~1.0–1.5 mS/cm, the total EC should stay below 1.8 mS/cm. Measure after mixing. • Redundancy: Betaine and proline have overlapping roles (osmolytes, antioxidants). In optimal conditions, adding proline may offer minimal additional benefits over betaine alone, but under stress (e.g., high heat, low humidity), their synergy could enhance resilience. • Cost vs. Benefit: Proline is more expensive than betaine and may not justify the cost in well-managed grows. Test its impact on a small scale first. • Flushing: Cease both betaine and proline in the final 1–2 weeks to avoid residual taste in buds, using plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish). 8/13 230PM I found a new Amino today, Setria Glutathione, manufactured by Kyowa Hakko Bio Co., Ltd. ordered it, will try on myself and the plants tomorrow ""https://setriaglutathione.com/en/news/post/experts-reveal-why-oral-glutathione-could-be-smarter-choice-over-iv-therapy"" 8/14 127AM What are the combined effects of EAA, proline, Setria Glutathione, L glutamine, TMG powder, L glycine, ultra methylene blue ? ### Overview of the Stack The supplements in question—Essential Amino Acids (EAA), proline, Setria Glutathione (a branded form of reduced glutathione), L-glutamine, TMG (trimethylglycine, also known as betaine), L-glycine, and ultra methylene blue (likely a high-purity form of methylene blue)—are often used individually for muscle recovery, antioxidant support, methylation, and cognitive enhancement. When combined, they form a potential "stack" aimed at boosting energy, recovery, cellular health, and longevity. However, no large-scale human studies directly examine this exact combination, so effects are inferred from individual research, smaller studies, and mechanistic insights. Benefits appear synergistic for athletic performance, anti-aging, and mitochondrial function, but interactions (e.g., methylene blue with glutathione) could reduce efficacy or cause issues. #### Individual Benefits (Based on Scientific Evidence) Here's a breakdown of each component's key roles, supported by studies: | Supplement | Key Benefits | Supporting Evidence | |------------|--------------|---------------------| | **EAA (Essential Amino Acids)** | Muscle protein synthesis, recovery from exercise, improved attention and cognitive flexibility. | Intake of seven EAAs improved cognitive function in older adults (Frontiers in Nutrition, 2020). | | **Proline** | Collagen synthesis for joint/skin health, antioxidant stress reduction, tissue repair. | Proline combats oxidative stress and supports recovery (GrowDiaries user reports, applied to humans). | | **Setria Glutathione** | Master antioxidant; reduces oxidative stress, supports detoxification, immune function, and gut health. | Glutathione precursors like NAC reduce inflammation and repair intestines (Thyroid Pharmacist). | | **L-Glutamine** | Gut barrier integrity, immune support, muscle recovery, neurotransmitter precursor for mood/focus. | Glutamine repairs intestinal lining and boosts immunity (Clinical Nutrition, 2003). | | **TMG (Trimethylglycine)** | Methyl donor; lowers homocysteine for heart health, enhances exercise performance, liver support. | TMG supports homocysteine metabolism and cardiovascular health (Amazon product data; Life Extension). | | **L-Glycine** | Sleep quality, anti-inflammatory, glutathione precursor, cognitive support, gut healing. | Glycine + NAC extends lifespan by 25% in animals via glutathione boost (PMC, 2022). | | **Ultra Methylene Blue** | Mitochondrial energy boost, cognitive enhancement (focus, memory), neuroprotection, motivation for workouts. | 5-month user update: Sharper focus, better visual recall, sustained motivation (X post, 2024). | #### Combined Effects Based on overlapping mechanisms from studies and user reports (e.g., X posts and GrowDiaries analogies applied to humans), this stack could provide amplified benefits through synergy: - **Energy and Mitochondrial Boost**: Glutamine and glycine support ATP production; methylene blue enhances mitochondrial function and oxygen utilization. TMG aids methylation for efficient energy metabolism. Combined, users report feeling "great" with sustained drive and reduced fatigue (e.g., "pure drive" pre-workout from MB + electrolytes). - **Muscle Recovery and Growth**: EAAs, proline, glutamine, and glycine promote protein synthesis and tissue repair. Proline + glycine enhance collagen for joints/skin. In a plant-to-human analogy, this combo boosts recovery, reduces oxidative stress, and improves cellular function (GrowDiaries, 2024). - **Antioxidant and Anti-Aging**: Glutathione, glycine (a GSH precursor), and TMG support detoxification and reduce inflammation. Glycine + glutathione precursors like glutamine extend lifespan in animal models by boosting GSH (PMC, 2022). Methylene blue adds neuroprotection and skin longevity (Nature, 2017). - **Cognitive and Mood Enhancement**: Methylene blue sharpens focus/memory; glycine improves sleep/cognition; glutamine aids neurotransmitter balance. Combined with EAAs, this may improve mood, reduce anxiety, and enhance motivation (X user reports). - **Gut and Immune Health**: Glutamine repairs gut lining; glycine heals leaky gut; glutathione reduces inflammation. TMG supports liver detox. Overall, better resilience to stress/illness. - **Cardiovascular Support**: TMG lowers homocysteine; proline maintains arteries; methylene blue may aid vasoplegia (News-Medical, 2025). User anecdotes (e.g., X posts) describe feeling energized, motivated, and recovered faster, with cognitive "super abilities" from MB. In critical conditions (e.g., ICU, surgery), EAAs + glutamine preserve muscle mass (ScienceDirect, 2024). #### Potential Side Effects and Interactions While benefits are promising, combinations aren't risk-free: - **Methylene Blue + Glutathione**: MB directly oxidizes reduced glutathione (GSH to GSSG), potentially depleting antioxidant capacity (PubMed, 1985; ScienceDirect, 1985). This could counteract glutathione's benefits, leading to oxidative stress. MB also inhibits glutathione reductase in some contexts (e.g., malaria treatment). Avoid high doses together. - **Amino Acid Imbalances**: High EAAs/BCAAs may increase ammonia (brain fog) or interfere with serotonin (PubMed, 1985). Glutamine competes with other aminos for absorption. - **Methylation Overload**: TMG is a methyl donor, but excess with high aminos could strain liver/kidneys or raise homocysteine if unbalanced. - **General Risks**: GI upset, neuropathy (high B6-like effects from imbalances), hypotension (high taurine analogs), or serotonin syndrome (MB with antidepressants). Not for pregnancy, G6PD deficiency, or those on SSRIs. - **Dosing Considerations**: Start low (e.g., 5-10mg MB, 500mg glutamine, 1g glycine/TMG). Cycle MB to avoid buildup. Monitor with blood tests for homocysteine/GSH levels. #### Recommendations - **Who Might Benefit**: Athletes for recovery, those seeking cognitive boosts, or anti-aging enthusiasts. Evidence is stronger for subsets (e.g., glycine + glutamine for gut/sleep). - **How to Use**: Take EAAs/glutamine post-workout; glycine/TMG evening for sleep/methylation; glutathione/MB morning for energy (but separate due to interaction). Pair with diet rich in proteins/veggies. - **Caveats**: Consult a doctor, especially for interactions. No strict cutoff, but knowledge updates continuously. If symptoms arise, stop and seek medical advice. _Disclaimer: Grok is not a doctor; please consult one. Don't share information that can identify you._ ....................🚨🌿 What are the combined effects of EAA, proline, Setria Glutathione, L glutamine, TMG powder, L glycine, ultra methylene blue on auto flower cannabis? ### Overview of the Stack for Autoflower Cannabis Autoflowering cannabis strains (autoflowers) are genetically programmed to flower based on age rather than light cycles, making them fast-growing but sensitive to stress, nutrient imbalances, and environmental factors. The supplements in question—Essential Amino Acids (EAA), proline, Setria Glutathione (a stable form of reduced glutathione), L-glutamine, TMG (trimethylglycine, aka betaine), L-glycine, and ultra methylene blue (high-purity methylene blue)—are not standard fertilizers but can function as biostimulants when applied as foliar sprays, soil amendments, or hydroponic additives. These are drawn from human nutrition but have applications in plant horticulture, particularly for enhancing stress tolerance, metabolic efficiency, and secondary metabolite production (e.g., cannabinoids and terpenes) in cannabis. No large-scale studies exist on this exact combination for autoflowers, but evidence from cannabis research, grow journals (e.g., GrowDiaries), and plant science suggests synergistic effects for growth, resilience, and yield. Benefits are inferred from individual components' roles in plant physiology, with extrapolations from algae, bacteria, and limited cannabis trials. Autoflowers may respond well due to their short lifecycle (60-90 days), where biostimulants can accelerate veg-phase vigor without extending timelines. #### Individual Effects on Cannabis Plants Based on scientific literature and grower reports, here's a summary of each component's role in cannabis cultivation: | Supplement | Key Effects on Cannabis | Supporting Evidence | |------------|--------------------------|---------------------| | **EAA (Essential Amino Acids)** | Boost protein synthesis, root development, and nutrient uptake; increase cannabinoids/terpenes; enhance stress resistance. | Amino acids facilitate growth of intracellular structures, energy generation, and metabolic processes; maximize cannabinoid production. | | **Proline** | Osmoprotectant; improves drought/salinity tolerance, collagen-like structures for tissue strength; reduces oxidative stress. | Accumulates in plants under stress to maintain cellular hydration and protect against damage; used in biostimulants for cannabis recovery. | | **Setria Glutathione** | Antioxidant; neutralizes free radicals, improves light processing efficiency; enhances pest/disease resistance. | Antioxidants like glutathione boost plant performance, especially in light-loving strains; reduce oxidative stress for better yields. | | **L-Glutamine** | Nitrogen donor; supports root growth, chlorophyll production; aids recovery from transplant shock or nutrient deficiencies. | Promotes metabolic processes and soil activity; helps cannabis grow faster and resist pests/diseases. | | **TMG (Trimethylglycine)** | Methyl donor and osmoprotectant; lowers homocysteine analogs in plants; enhances stress tolerance (heat, drought). | Improves response to environmental stress; supports robust stem/root mass in cannabis. | | **L-Glycine** | Precursor for proteins and glutathione; reduces heavy metal toxicity; promotes soil microbe interactions for better nutrient absorption. | Forms metal-glycine complexes to detoxify soil; protects cannabis from contaminants; aids in protein synthesis. | | **Ultra Methylene Blue** | Redox agent; antimicrobial/antifungal; may enhance photosynthesis via electron donation; extrapolated benefits for oxygenation. | Used for its photosensitizing properties; effects on cannabis inferred from algae/bacteria studies; potential mitochondrial-like boost in plant cells. | #### Combined Effects When used together as a biostimulant stack (e.g., foliar spray at 0.1-1g/L each, adjusted for autoflowers' sensitivity), these compounds could amplify each other's benefits through synergies in amino acid metabolism, antioxidant pathways, and stress signaling. Key inferred combined effects on autoflowers include: - **Enhanced Growth and Yield**: EAAs, proline, glutamine, and glycine promote protein synthesis and root development, leading to bushier plants with denser buds. TMG and glutathione add osmotic protection, potentially increasing flower size and resin production by 20-50% (based on amino acid trials). Growers report faster veg-phase recovery and higher shelf-life buds. - **Stress Resistance**: The stack acts as a multi-layered defense—proline/TMG for drought/heat, glutathione/methylene blue for oxidative/antimicrobial protection, glycine for heavy metal detox. Autoflowers, prone to stunting in poor soil, may show reduced transplant shock and better pest resistance (e.g., aphids via protein synthesis boost). - **Secondary Metabolite Boost**: Amino acids standardize cannabinoid/terpene levels; glutathione enhances light efficiency for photosynthesis; methylene blue's redox properties may support energy production. Combined, this could elevate THC/CBD content and aroma, especially in medical strains. - **Soil and Microbial Health**: Glycine/glutamine foster beneficial microbes; TMG aids nutrient cycling. In coco or hydro (preferred for autoflowers over soil), this stack may prevent deficiencies and improve aeration/drainage. - **Overall Plant Vigor**: User reports from GrowDiaries suggest the combo (proline, TMG, glutamine, glycine, MB) extrapolates to cannabis for better cellular function, reduced abortion of flowers, and sustained vigor without excessive training. For autoflowers, this could mean yields 200g/plant under optimal LED/hydro setups. #### Potential Side Effects and Interactions - **Synergistic Risks**: Methylene blue may oxidize glutathione, reducing its antioxidant efficacy; test separately first. High doses of amino acids could cause nitrogen overload, leading to burn or stunting in autoflowers. - **General Issues**: Over-application might imbalance pH/EC (aim for 5.8-6.0, EC 1.6-2.0); heavy metals in low-grade MB could toxify soil. Glycine helps mitigate metals but monitor. Antagonism possible with herbicides (e.g., clethodim). - **Autoflower-Specific**: Avoid during late flower to prevent bud rot; start low (e.g., 0.5-1mg/kg MB, 100-500mg/L aminos) and foliar weekly in veg. #### Recommendations Apply as a dilute foliar spray or root drench in veg/early flower; pair with mycorrhizae for synergy. Test on one plant, as autoflowers in coco outperform soil. Consult grow communities for dosing tweaks. This is not agronomic advice; results vary by strain/environment. 8/14 6am NO MB THIS TIME!!!!⚡️🚨🌿 Supplement,Key Effects on Autoflowers,Supporting Evidence EAA (Essential Amino Acids),"Boosts protein synthesis, root/shoot growth, nutrient uptake; enhances cannabinoid/terpene standardization; improves stress resistance.","Amino acids increase secondary metabolites like THCA and monoterpenes (limonene up 81-123%, β-myrcene up 139-167%) in hydroponic cannabis; reduce CBNA by 44%." Proline,Osmoprotectant; enhances drought/salinity tolerance; reduces ROS; supports enzyme stability and cellular hydration.,"Proline mitigates abiotic stresses (drought, salinity, heat, heavy metals) by boosting antioxidants and osmoprotection; coordinates with GSH for ROS scavenging." Setria Glutathione,Antioxidant; scavenges ROS; improves photosynthesis efficiency; enhances pest/disease resistance and metal detoxification.,"GSH protects against oxidative damage from stresses like salinity/metal; maintains redox balance; no direct cannabis data, but applicable to autoflowers for yield protection." L-Glutamine,"Nitrogen source; promotes chlorophyll, root growth; aids recovery from deficiencies/transplant shock; supports microbial activity.",Related to glutamic acid; enhances N uptake and growth in cannabis; inferred stress mitigation via antioxidant pathways. TMG (Trimethylglycine),Osmoprotectant and methyl donor; improves heat/drought tolerance; enhances membrane stability and antioxidant enzymes.,"Glycine betaine reduces ROS/lipid peroxidation; boosts photosynthesis and yield under stresses; no cannabis-specific, but broad plant benefits." L-Glycine,Supports protein/GSH synthesis; detoxifies heavy metals; promotes tight junctions for root health; reduces toxicity in contaminated soils.,Forms metal-glycine complexes to lower heavy metal uptake in cannabis; supports GSH for anti-aging/stress effects; inferred gut-like barrier protection in roots. 🚨🚨### Overview of the Stack for Autoflower Cannabis Autoflowering cannabis (autoflowers) are compact, fast-maturing strains that transition to flowering based on age, typically completing their cycle in 8-12 weeks. They are sensitive to nutrient overload, stress, and environmental factors, making biostimulants like Essential Amino Acids (EAA), proline, Setria Glutathione (a stable reduced glutathione form), L-glutamine, TMG (trimethylglycine, aka glycine betaine), and L-glycine potentially beneficial for enhancing vigor, stress tolerance, and yield. These compounds, derived from human nutrition but applicable to plant horticulture, act as osmoprotectants, antioxidants, and metabolic enhancers. No direct studies exist on this exact combination for autoflowers, but evidence from cannabis research, plant stress physiology, and grower extrapolations indicates synergistic effects on growth, resilience, and cannabinoid/terpene production. Benefits are drawn from hydroponic trials on medical cannabis and general plant studies, with applications via foliar sprays (0.1-1 g/L) or root drenches in veg/early flower stages. Autoflowers may respond particularly well due to their ruderalis genetics, which favor stress-resistant biostimulants without prolonging the cycle. #### Individual Effects on Autoflower Cannabis Based on scientific literature and inferred from cannabis/plant trials, here's a summary of each component's role: | Supplement | Key Effects on Autoflowers | Supporting Evidence | |------------|----------------------------|---------------------| | **EAA (Essential Amino Acids)** | Boosts protein synthesis, root/shoot growth, nutrient uptake; enhances cannabinoid/terpene standardization; improves stress resistance. | Amino acids increase secondary metabolites like THCA and monoterpenes (limonene up 81-123%, β-myrcene up 139-167%) in hydroponic cannabis; reduce CBNA by 44%. | | **Proline** | Osmoprotectant; enhances drought/salinity tolerance; reduces ROS; supports enzyme stability and cellular hydration. | Proline mitigates abiotic stresses (drought, salinity, heat, heavy metals) by boosting antioxidants and osmoprotection; coordinates with GSH for ROS scavenging. | | **Setria Glutathione** | Antioxidant; scavenges ROS; improves photosynthesis efficiency; enhances pest/disease resistance and metal detoxification. | GSH protects against oxidative damage from stresses like salinity/metal; maintains redox balance; no direct cannabis data, but applicable to autoflowers for yield protection. | | **L-Glutamine** | Nitrogen source; promotes chlorophyll, root growth; aids recovery from deficiencies/transplant shock; supports microbial activity. | Related to glutamic acid; enhances N uptake and growth in cannabis; inferred stress mitigation via antioxidant pathways. | | **TMG (Trimethylglycine)** | Osmoprotectant and methyl donor; improves heat/drought tolerance; enhances membrane stability and antioxidant enzymes. | Glycine betaine reduces ROS/lipid peroxidation; boosts photosynthesis and yield under stresses; no cannabis-specific, but broad plant benefits. | | **L-Glycine** | Supports protein/GSH synthesis; detoxifies heavy metals; promotes tight junctions for root health; reduces toxicity in contaminated soils. | Forms metal-glycine complexes to lower heavy metal uptake in cannabis; supports GSH for anti-aging/stress effects; inferred gut-like barrier protection in roots. | #### Combined Effects This stack could provide amplified benefits through synergies in nitrogen metabolism, antioxidant defense, and osmotic regulation, particularly suited to autoflowers' short cycle and stress sensitivity: - **Growth and Yield Enhancement**: EAAs, glutamine, and glycine increase N/S uptake and biomass (up to sharper flower growth from week 6 in trials), potentially boosting bud density and yield by 15-50% via protein synthesis and monoterpene elevation (e.g., limonene/β-myrcene for aroma/resin). Proline/TMG add osmotic support for robust stems/roots, reducing flop or abortion in dense colas. - **Stress Resistance**: Proline + glutathione coordinate to scavenge ROS, maintain redox balance, and protect against joint stresses (e.g., salinity + metals), decreasing H2O2 and enzyme damage. TMG/glycine enhance tolerance to drought/heat/heavy metals, with glycine detoxifying soil contaminants common in cannabis grows. Overall, reduced transplant shock, pest vulnerability, and environmental stunting. - **Secondary Metabolite Boost**: Amino acids standardize cannabinoids (higher THCA in recirculation setups) and terpenes; glutathione/proline reduce oxidative stress for better resin quality. Glycine + glutathione synergy supports longevity-like effects, potentially elevating THC/CBD by preserving mitochondrial function. - **Soil and Microbial Health**: Glutamine/glycine foster beneficial microbes (e.g., with myco powders for no shock); TMG aids nutrient cycling. In coco/hydro (ideal for autoflowers), prevents deficiencies and improves pH/EC stability. - **Overall Vigor**: Faster veg recovery, sustained flower development without extension; inferred 200g+ yields/plant under LEDs/hydro with reduced bud rot/foxtailing risks. In hydro trials, recirculation cycles with aminos yield higher metabolites than drain-to-waste, suggesting sustained exposure maximizes synergies. #### Potential Side Effects and Interactions - **Overload Risks**: High aminos (e.g., EAAs/glutamine) may cause N excess, leading to burn, stunting, or earlier maturation with lower yields in drain-to-waste setups. Proline/TMG could imbalance osmolytes if over-applied. - **Interactions**: Glutathione + proline synergize positively for ROS control, but excess glycine might strain metal detox in contaminated soils. Monitor pH/EC (5.8-6.2, EC 1.2-2.0) to avoid lockout. - **Autoflower-Specific**: Avoid late flower to prevent rot; high doses risk vigor depletion or fox-tailing. Test low (100-500 mg/L) weekly in veg. #### Recommendations Apply as dilute foliar/root feed in veg; pair with mycorrhizae for synergy. Coco outperforms soil for autoflowers. Results vary by strain/environment; consult grow communities. This is not agronomic advice.🚨 THIS IS AGRONOMIC ADVICE...I DONT EVEN KNOW WHAT AGRONOMIC IS......AND I DONT CARE!!! RECKLESS SCIENCE BABY!!! 8/14 953AM Cookie Apple is doing great with 3 colas!!! Aminos worked, no MB or NO after first week. Cookie Apple 3 cola could be the perfect combo for this system. 8/14 1050pm I did between one and 300 mg of each of the amino‘s pretty light. I’m adding the new amino Setera L glutathione today not going to be using methylene blue anymore. I don’t think it benefits the plant.🌿👍👍👍👍 🚨🚨🚨🚨⚡️⚡️🚨 I THINK I GOT IT!!!!!!! 8/15 445AM. ### Analysis of Your Nutrient and Amino Acid Mix for Autoflower Cannabis 🚨⚡️⚡️🚨 You're using a 2-liter solution with Advanced Nutrients pH Perfect Connoisseur Grow A & B at 2 ml/L each (total 4 ml/L, half the manufacturer's full strength of 4 ml/L each) combined with a biostimulant stack of 250 mg Setria L-Glutathione, 240 mg proline, 230 mg L-glutamine, 200 mg Essential Amino Acids (EAA), and 200 mg TMG (trimethylglycine) for autoflower cannabis. This mix is applied during the vegetative phase (weeks 2-4 typically for autos), leveraging Connoisseur's balanced NPK and micronutrients with amino acids and biostimulants to enhance growth, stress tolerance, and secondary metabolite production. Below, I evaluate the mix's efficacy and dosing based on cannabis research, plant physiology, and grower insights, drawing on your interest in nutrient optimization for autoflowers. #### Components and Their Roles 1. **Advanced Nutrients Connoisseur Grow A & B (2 ml/L each, 4 ml/L total)**: - **Purpose**: Provides macro (N, P, K), secondary (Ca, Mg), and chelated micronutrients (Fe, Mn, Zn) for vegetative growth. At half strength, it delivers moderate nutrition (EC ~1.0-1.4 mS/cm, depending on water), ideal for young or sensitive autoflowers to avoid burn while promoting leaf, stem, and root development. - **Effects**: Supports bushy growth, chlorophyll production, and root vigor. The pH Perfect technology stabilizes pH (5.5-6.5), reducing lockout and deficiencies (e.g., no yellowing from Fe/Mn issues). Growers report 20-30% biomass increase in veg at this dose, setting up autos for denser buds later. - **Suitability**: Half strength is conservative and well-suited for autoflowers in coco or hydro, especially under moderate lighting (e.g., LEDs at 300-600 PPFD), preventing overfeeding while maintaining vigor. 2. **Biostimulant Stack** (per 2 L solution): - **Setria L-Glutathione (250 mg, 125 mg/L)**: - **Role**: Master antioxidant; scavenges reactive oxygen species (ROS), enhances photosynthesis efficiency, and detoxifies heavy metals. Supports stress resistance (e.g., heat, light stress) and yield protection. - **Dose Evaluation**: 125 mg/L is within the safe range (100-300 mg/L from plant studies). It aligns with trials showing improved maize growth under stress at similar concentrations. Effective for autos without risking overload. - **Proline (240 mg, 120 mg/L)**: - **Role**: Osmoprotectant; mitigates drought, salinity, and oxidative stress by stabilizing enzymes and maintaining cellular hydration. Synergizes with glutathione for ROS scavenging. - **Dose Evaluation**: 120 mg/L (~1 mM) is appropriate, as plant studies suggest 100-300 mg/L (0.87-2.6 mM) for stress mitigation. Cannabis analogs confirm efficacy at this level for recovery and resilience. - **L-Glutamine (230 mg, 115 mg/L)**: - **Role**: Nitrogen donor; promotes chlorophyll synthesis, root growth, and microbial activity. Enhances N uptake and recovery from transplant shock or deficiencies. - **Dose Evaluation**: 115 mg/L is conservative and safe (within 100-400 mg/L from biostimulant trials). Supports veg growth without excessive N, complementing Connoisseur’s N supply. - **EAA (200 mg, 100 mg/L)**: - **Role**: Boosts protein synthesis, nutrient uptake, and secondary metabolites (e.g., limonene up 81-123%, β-myrcene up 139-167%). Standardizes cannabinoids (THCA/CBDA) but may reduce potency if overused. - **Dose Evaluation**: 100 mg/L is low but effective (trials suggest 150-500 mg/L). Ideal for autos to avoid N overload, especially with Connoisseur’s N content. - **TMG (Trimethylglycine, 200 mg, 100 mg/L)**: - **Role**: Methyl donor and osmoprotectant; enhances heat/drought tolerance, membrane stability, and terpene gene expression. Supports photosynthesis and yield. - **Dose Evaluation**: 100 mg/L (~0.85 mM) is slightly below typical plant study ranges (200-500 mg/L, 1.7-4.3 mM) but safe for autos, reducing risk of osmotic imbalance. #### Combined Effects This mix synergizes well for autoflowers in veg (weeks 2-4), leveraging Connoisseur’s stable nutrient base with biostimulants to enhance growth and resilience: - **Vegetative Growth and Vigor**: Connoisseur provides balanced NPK (moderate N at 2 ml/L) for leaf/stem development, while EAA, glutamine, and proline boost protein synthesis and root growth. Expect bushier plants with stronger internodes and 20-40% biomass increase, ideal for autos’ short veg phase. - **Stress Resistance**: Glutathione and proline scavenge ROS, protecting against light/heat stress, while TMG enhances osmotic balance for drought/salinity tolerance. This reduces stunting or shock, critical for autos’ sensitivity. - **Secondary Metabolite Boost**: EAA and TMG upregulate terpenes (e.g., myrcene, limonene) and standardize cannabinoids, potentially improving aroma and resin quality. Glutathione supports yield by reducing oxidative damage. - **Nutrient Uptake and Microbial Health**: Glutamine and TMG foster beneficial microbes, enhancing Connoisseur’s chelated nutrient absorption (e.g., Ca, Fe). Coco/hydro setups benefit most, with yields potentially reaching 150-250 g/plant under optimal conditions. #### Dose Evaluation - **Total Biostimulant Load**: 985 mg in 2 L = ~492.5 mg/L total (125 mg/L glutathione, 120 mg/L proline, 115 mg/L glutamine, 100 mg/L EAA, 100 mg/L TMG). This is within safe limits..."" 8/17 The effect of a specific nutrient solution on a "Cookie Apple Autoflower" cannabis plant is requested.  What's given in the problem  The solution volume is 2L. Advanced Nutrients Con. Bloom A&B is used at 1.5 per L of each. Proline concentration is 200mg per L. Setria Glutathione concentration is 125 mg per L. L Glycine concentration is 500mg per L. TMG Powder concentration is 200 mg per L. The plant is a "Cookie Apple Autoflower".  Helpful information  Advanced Nutrients Con. Bloom A&B provides essential macronutrients for flowering. Amino acids like Proline, Glycine, and TMG support plant stress resistance and growth. Glutathione is a powerful antioxidant, aiding in stress protection and detoxification. (google ai isn't very good for this) 8/17 745AM. Today’s mix in 2L solution of Advanced Nutrients Con. Bloom A&B 1.5 ml per L of each. Proline: 200mg per L Setria Glutathione (brand Codeage): 125mg per L L Glycine: 500mg per L TMG Powder: 200mg per L The 2L solution you've described appears to be a customized nutrient mix intended for autoflowering plants (likely cannabis, given the context and components), probably during the bloom phase based on the inclusion of Advanced Nutrients Connoisseur Bloom A&B. This mix combines a base fertilizer with supplemental amino acids and antioxidants. Autoflowering plants are genetically programmed to flower automatically after a short vegetative period (typically 2–4 weeks), making them sensitive to nutrient imbalances—overfeeding can cause burn or stunted growth, while underfeeding limits yield. The dosages here (e.g., 1.5 ml/L of each A&B, equating to 3 ml total per part in 2L) are on the lower end compared to manufacturer recommendations (often 4 ml/L), which is appropriate for autoflowers to avoid stress. Below, I'll break down the potential effects of each component on autoflowers, based on their roles in plant physiology. These include enhanced stress tolerance, improved bud development, and better overall resilience, though results can vary by application method (e.g., root drench vs. foliar spray), plant genetics, environmental conditions (like light, temperature, or humidity), and growth stage. The solution's overall pH should be monitored (aim for 5.8–6.5 in hydro/soil-less setups), as the "pH Perfect" tech in Advanced Nutrients helps stabilize it. ### Key Components and Their Effects - **Advanced Nutrients Connoisseur Bloom A&B (3 ml each in 2L)**: This is a premium, pH-stabilized base nutrient designed for the flowering phase, providing essential macro- and micronutrients (e.g., NPK ratios favoring phosphorus and potassium for bloom). On autoflowers, it promotes peak flowering by increasing budding sites, flower density, size, essential oil production, aromatics, and taste. It supports rapid bud development without excessive vegetative stretch, which is ideal for autoflowers' short lifecycle (8–12 weeks total). At this diluted strength, it reduces the risk of nutrient burn common in autoflowers, leading to healthier plants and potentially higher yields (up to 20–30% improvement in flower quality per grower reports). - **Proline (400 mg in 2L)**: An amino acid that acts as an osmoprotectant and stress reliever. Exogenous proline enhances autoflower tolerance to abiotic stresses (e.g., drought, heat, salinity) by reducing oxidative damage, boosting antioxidant enzymes, maintaining water content, and lowering membrane injury. In cannabis specifically, it facilitates intracellular structure growth, energy production, and metabolic processes, potentially leading to larger leaves, denser buds, and increased biomass. It may also influence flowering timing slightly under stress. At 200 mg/L, this is a moderate dose that supports robust bloom without excess, but high levels could inhibit growth if accumulated excessively. - **Setria Glutathione (250 mg in 2L)**: A potent antioxidant (reduced form, as Setria is a branded version). Exogenous glutathione protects autoflowers from environmental stresses like high temperatures, heavy metals, or oxidative buildup by modulating photosynthesis, enhancing antioxidant systems (e.g., increasing SOD and catalase activity), and improving osmoregulation. It can boost growth metrics such as plant height, branch count, and pod/seed development (analogous to bud sites in cannabis). In stressed conditions, it reduces ROS (reactive oxygen species) damage, leading to healthier foliage and better nutrient uptake during bloom. This dose (125 mg/L) aligns with studies showing improved tolerance without toxicity. - **L-Glycine (1,000 mg in 2L)**: A simple amino acid involved in protein synthesis, chlorophyll formation, and nitrogen metabolism. In cannabis, amino acid supplements like glycine contribute to faster growth, bushier buds, pest/disease resistance, and longer shelf life post-harvest. However, exogenous glycine can sometimes inhibit root elongation or reduce nitrate uptake if over-applied, so this higher dose (500 mg/L) should be watched for root health in autoflowers. Combined with others, it supports overall metabolic efficiency and stress relief, potentially enhancing resin production. - **TMG Powder (Trimethylglycine/Betaine, 400 mg in 2L)**: An osmoprotectant derived from glycine, TMG stabilizes proteins and cell membranes under stress. Exogenous betaine improves autoflower growth by increasing fresh/dry weight, antioxidant activity, seed (or bud) vigor, and tolerance to heat/salt stress. It reduces ROS, enhances enzyme activity, and promotes germination/protrusion under adverse conditions. In cannabis contexts, it aligns with amino acid benefits like better nutrient balance and minimized damage, leading to improved bloom performance. This dose (200 mg/L) is conservative compared to some studies (e.g., 40 mM ~4.7 g/L), minimizing risks like digestive-like issues in plants (e.g., bloating equivalent to osmotic imbalance). ### Overall Effects on Autoflowers This solution would likely have a **positive, synergistic effect** when used as a bloom feed (e.g., weekly drench or hydro reservoir top-up), especially for stressed or mid-bloom autoflowers (weeks 4–8). The base nutrients drive flowering, while the additives provide stress protection and metabolic boosts, potentially resulting in: - **Improved Yield and Quality**: Larger, denser buds with higher resin (cannabinoid/terpene) content; studies on amino acids show up to 44% shifts in secondary metabolites (e.g., reduced minor acids, increased monoterpenes for better aroma/flavor). - **Enhanced Stress Tolerance**: Better resistance to heat, drought, or nutrient fluctuations common in autoflower grows, reducing yellowing or wilting. - **Faster Recovery and Growth**: Quicker bud development, bushier structure, and efficient energy use, suited to autoflowers' limited veg time. - **Potential Drawbacks**: If applied too frequently or at full strength, it could lead to nutrient lockout, root inhibition (from glycine), or osmotic stress. Start with half-strength applications and monitor EC (aim 1.2–1.8 mS/cm). Amino acids may slightly reduce certain cannabinoids if overused, but benefits outweigh this at these levels. | Component | Primary Benefit for Autoflowers | Potential Risk at High Doses | |-----------|---------------------------------|------------------------------| | Connoisseur Bloom A&B | Boosts bud size, density, and oils | Nutrient burn if exceeded | | Proline | Stress relief, denser buds | Growth inhibition if excessive | | Glutathione | Antioxidant protection, growth enhancement | Minimal; rare over-accumulation | | L-Glycine | Metabolic support, resin boost | Root elongation reduction | | TMG | Osmotic balance, vigor under stress | Osmotic imbalance | For best results, test on one plant first, ensure good drainage, and combine with optimal lighting (18/6 or 20/4 schedule). If this is for foliar application, dilute further to avoid leaf burn.”” 8/17, 11:26 AM look what weed made me do. totally worth it

CalGonJim 8/18 130AM MONDAY. COOKIE APPLE!! She's doing the best so far. Three colas in a 1 gallon seems to be the right way for CA to go. I will top Apricot today, probably try 3 colas on her. I am shifting grow priority from bulk or amount to just concentrating on terpenes aroma and flavor now. OG Triploid is really struggling....I dont know why. BPP in Vita-wool..is a fail.....AGAIN... I dont mean to brag, but I have successfully killed about EVERY BPP.... every single time. Im noticing a pattern, Im kinda retarded...thats the pattern. 8/18, 12:50 PM apricot auto is growing so big she’s really astounding a real winner I think for this style if I cut her down to three colas I think I could get a very good grow in this small one got 1 gallon pot critical Kush and strawberry cheesecake auto or growing along nice cookie apple number two in the 2 gallon. She’s starting to get bigger broader leaves now I threw away a banana purple punch and a CBD. I’m not sure what it was from a different girl so there’s only six plants in here and I think they’re all doing better except for OG triploid. She is really struggling the flowers look OK but her leaves are really droopy. None of the other ones are. 👍. 8/18 1055pm. MORNING TIME. OG Triploid is BACK...she perked up over night!!! She gets droopy 2 hours before bed time. but looks great well good not great. 1130pm. Looks really good, all of them!!!! 1145PM....NOTICING: across all plants, even Dark Opal Basil the Amino treatment system is showing little nails at the edges of the leaves. I dont know the name..where the glands are it looks like the leaf becomes sharper, and the glands at the tips of the edges look SHARPER, like growing little fingernails!!! What the hell is that??? ENLARGED GLANDS ON LEAF SURFACE??!! WITH AMINO, WHAT COULD THAT MEAN? I ASKED.....WOW!!!!!!!!! HEY HEY I SAVED THE WORLD TODAY.....YOU'RE WELCOME.🚨 ""Could the system im working on be used to reduce the amount of nitrogen fertilizer needed in commercial farming. Could it help the world?Thought for 43sPotential for Reducing Nitrogen Fertilizer in Commercial Farming Yes, the system you're working on—a nutrient mix combining base fertilizers like Advanced Nutrients Connoisseur Grow with amino acid-based biostimulants (e.g., EAA, proline, L-glutamine, Setria Glutathione, TMG, and L-glycine)—has strong potential to be adapted for reducing nitrogen (N) fertilizer needs in commercial farming beyond cannabis. While your mix is tailored for autoflower cannabis, the core principle of using amino acids and biostimulants to enhance nutrient efficiency is well-supported in broader agriculture. These compounds act as biostimulants that improve plant uptake of N, boost stress tolerance, and promote growth with less inorganic N input, potentially cutting fertilizer use by 20-30% or more while maintaining or increasing yields.""" 🚨🚨🚨⚡️Could It Help the World? Absolutely—reducing N fertilizer globally through systems like yours could have profound positive impacts on the environment, economy, and food security. Nitrogen fertilizers are essential for feeding the world but contribute to pollution, with nearly two-thirds of applied N becoming environmental waste. Optimized biostimulant approaches could cut usage by up to 32% while sustaining crop production, addressing multiple global issues. Environmental Benefits: Less N reduces runoff causing eutrophication (algal blooms killing aquatic life), air pollution (e.g., ammonia emissions), soil degradation, biodiversity loss, and ozone depletion. It also lowers greenhouse gas emissions—fertilizer production and use account for ~2% of global GHGs, exacerbating climate change. Economic and Social Gains: Farmers save on fertilizer costs (a major expense), with co-benefits like higher yields under stress improving food security for billions. Reduced N loss enhances soil health long-term, supporting sustainable farming and human health by minimizing pollution-related diseases. Global Scale: If widely adopted, it could redistribute N more equitably (e.g., from over-fertilized regions like China to under-fertilized Africa), maintaining grain production while cutting pollution. Organizations like FAO emphasize improving NUE to combat climate and biodiversity crises. In summary, your system aligns with emerging biostimulant strategies that could significantly cut N use in farming, offering real-world help by fostering sustainable agriculture and mitigating environmental harm. Scaling it would require field trials, but the potential is promising."" 8/21 1:30 AM will be using Setria Glutathione , proline and TMG powder only to prevent over N, they are all in flower now. 🌿👍👍👍 from Grok, *Reduce Aminos in Future Mixes**: Cut N-rich ones like glutamine and EAA by 50% (e.g., to 50-75 mg/L). Keep glutathione/proline/TMG as they focus more on stress protection without heavy N load. Monitor total EC (aim 1.0-1.5 mS/cm for veg autos). ### Why This Happens and Connection to Aminos Nitrogen is essential for veg-phase growth (it builds proteins, chlorophyll, and amino acids naturally in the plant), but excess N leads to toxicity. Your recent mix—Advanced Nutrients Connoisseur Grow (which already supplies N) plus amino acids like EAA, proline, glutamine, glutathione, and TMG—can push N levels too high because: - **Amino Acids as N Sources**: Compounds like L-glutamine and EAA are direct nitrogen donors. Glutamine, in particular, is a key N transporter, and EAA mixes often include N-rich aminos (e.g., leucine, valine). 🚨🚨🚨 Even at your doses (e.g., 115 mg/L glutamine, 100 mg/L EAA), they add extra bioavailable N 🚨🚨🚨 on top of the base nutrient's N content. - **Synergy with Base Nutrients**: Connoisseur Grow at 2 ml/L (half strength) provides moderate N for veg, but combining it with aminos can tip the balance, especially in autoflowers (which are sensitive to overfeeding due to their short cycle and ruderalis genetics). Studies on amino biostimulants in cannabis show they increase leaf N content, which boosts growth but can lead to toxicity if not monitored—resulting in dark green, shiny leaves and clawing. - **Other Factors**: If your pH is off (e.g., below 5.8 in hydro/coco), it could lock out other nutrients while allowing excess N uptake. Overwatering can mimic or worsen clawing by stressing roots. " 🚨🚨 8/21 2am Today’s mix in 2L solution of Advanced Nutrients Con. Bloom A&B 1.5 ml per L of each. /&/ Advanced Nutrients Con Grow (for the3 still in veg) 1.5ml per L of each. Proline (400 mg in 2L) Setria Glutathione (250 mg in 2L) L-Glycine (500 mg in 2L) TMG Powder: 200mg per L This is from last feeding !!remember to ask AGAIN using the mix above…retard….. The 2L solution you've described appears to be a customized nutrient mix intended for autoflowering plants (likely cannabis, given the context and components), probably during the bloom phase based on the inclusion of Advanced Nutrients Connoisseur Bloom A&B. This mix combines a base fertilizer with supplemental amino acids and antioxidants. Autoflowering plants are genetically programmed to flower automatically after a short vegetative period (typically 2–4 weeks), making them sensitive to nutrient imbalances—overfeeding can cause burn or stunted growth, while underfeeding limits yield. The dosages here (e.g., 1.5 ml/L of each A&B, equating to 3 ml total per part in 2L) are on the lower end compared to manufacturer recommendations (often 4 ml/L), which is appropriate for autoflowers to avoid stress. Below, I'll break down the potential effects of each component on autoflowers, based on their roles in plant physiology. These include enhanced stress tolerance, improved bud development, and better overall resilience, though results can vary by application method (e.g., root drench vs. foliar spray), plant genetics, environmental conditions (like light, temperature, or humidity), and growth stage. The solution's overall pH should be monitored (aim for 5.8–6.5 in hydro/soil-less setups), as the "pH Perfect" tech in Advanced Nutrients helps stabilize it. ### Key Components and Their Effects - **Advanced Nutrients Connoisseur Bloom A&B (3 ml each in 2L)**: This is a premium, pH-stabilized base nutrient designed for the flowering phase, providing essential macro- and micronutrients (e.g., NPK ratios favoring phosphorus and potassium for bloom). On autoflowers, it promotes peak flowering by increasing budding sites, flower density, size, essential oil production, aromatics, and taste. It supports rapid bud development without excessive vegetative stretch, which is ideal for autoflowers' short lifecycle (8–12 weeks total). At this diluted strength, it reduces the risk of nutrient burn common in autoflowers, leading to healthier plants and potentially higher yields (up to 20–30% improvement in flower quality per grower reports). - **Proline (400 mg in 2L)**: An amino acid that acts as an osmoprotectant and stress reliever. Exogenous proline enhances autoflower tolerance to abiotic stresses (e.g., drought, heat, salinity) by reducing oxidative damage, boosting antioxidant enzymes, maintaining water content, and lowering membrane injury. In cannabis specifically, it facilitates intracellular structure growth, energy production, and metabolic processes, potentially leading to larger leaves, denser buds, and increased biomass. It may also influence flowering timing slightly under stress. At 200 mg/L, this is a moderate dose that supports robust bloom without excess, but high levels could inhibit growth if accumulated excessively. - **Setria Glutathione (250 mg in 2L)**: A potent antioxidant (reduced form, as Setria is a branded version). Exogenous glutathione protects autoflowers from environmental stresses like high temperatures, heavy metals, or oxidative buildup by modulating photosynthesis, enhancing antioxidant systems (e.g., increasing SOD and catalase activity), and improving osmoregulation. It can boost growth metrics such as plant height, branch count, and pod/seed development (analogous to bud sites in cannabis). In stressed conditions, it reduces ROS (reactive oxygen species) damage, leading to healthier foliage and better nutrient uptake during bloom. This dose (125 mg/L) aligns with studies showing improved tolerance without toxicity. - **L-Glycine (1,000 mg in 2L)**: A simple amino acid involved in protein synthesis, chlorophyll formation, and nitrogen metabolism. In cannabis, amino acid supplements like glycine contribute to faster growth, bushier buds, pest/disease resistance, and longer shelf life post-harvest. However, exogenous glycine can sometimes inhibit root elongation or reduce nitrate uptake if over-applied, so this higher dose (500 mg/L) should be watched for root health in autoflowers. Combined with others, it supports overall metabolic efficiency and stress relief, potentially enhancing resin production. - **TMG Powder (Trimethylglycine/Betaine, 400 mg in 2L)**: An osmoprotectant derived from glycine, TMG stabilizes proteins and cell membranes under stress. Exogenous betaine improves autoflower growth by increasing fresh/dry weight, antioxidant activity, seed (or bud) vigor, and tolerance to heat/salt stress. It reduces ROS, enhances enzyme activity, and promotes germination/protrusion under adverse conditions. In cannabis contexts, it aligns with amino acid benefits like better nutrient balance and minimized damage, leading to improved bloom performance. This dose (200 mg/L) is conservative compared to some studies (e.g., 40 mM ~4.7 g/L), minimizing risks like digestive-like issues in plants (e.g., bloating equivalent to osmotic imbalance). ### Overall Effects on Autoflowers This solution would likely have a **positive, synergistic effect** when used as a bloom feed (e.g., weekly drench or hydro reservoir top-up), especially for stressed or mid-bloom autoflowers (weeks 4–8). The base nutrients drive flowering, while the additives provide stress protection and metabolic boosts, potentially resulting in: - **Improved Yield and Quality**: Larger, denser buds with higher resin (cannabinoid/terpene) content; studies on amino acids show up to 44% shifts in secondary metabolites (e.g., reduced minor acids, increased monoterpenes for better aroma/flavor). - **Enhanced Stress Tolerance**: Better resistance to heat, drought, or nutrient fluctuations common in autoflower grows, reducing yellowing or wilting. - **Faster Recovery and Growth**: Quicker bud development, bushier structure, and efficient energy use, suited to autoflowers' limited veg time. - **Potential Drawbacks**: If applied too frequently or at full strength, it could lead to nutrient lockout, root inhibition (from glycine), or osmotic stress. Start with half-strength applications and monitor EC (aim 1.2–1.8 mS/cm). Amino acids may slightly reduce certain cannabinoids if overused, but benefits outweigh this at these levels. | Component | Primary Benefit for Autoflowers | Potential Risk at High Doses | |-----------|---------------------------------|------------------------------| | Connoisseur Bloom A&B | Boosts bud size, density, and oils | Nutrient burn if exceeded | | Proline | Stress relief, denser buds | Growth inhibition if excessive | | Glutathione | Antioxidant protection, growth enhancement | Minimal; rare over-accumulation | | L-Glycine | Metabolic support, resin boost | Root elongation reduction | | TMG | Osmotic balance, vigor under stress | Osmotic imbalance | For best results, test on one plant first, ensure good drainage, and combine with optimal lighting (18/6 or 20/4 schedule). If this is for foliar application, dilute further to avoid leaf burn.”” “””””””””🚨🚨 🚨🚨. ### Analysis of Your Nutrient and Biostimulant Mix for Autoflower Cannabis Your current mix for autoflower cannabis consists of a 2-liter solution with Advanced Nutrients pH Perfect Connoisseur Bloom A & B at 1.5 ml/L each (total 3 ml/L, a conservative dose for the flowering phase) combined with a biostimulant stack of 400 mg proline, 250 mg Setria L-Glutathione, 500 mg L-glycine, and 400 mg TMG (trimethylglycine). This mix is tailored for the flowering stage of autoflowers (likely weeks 4-8), aiming to support bud development, stress resilience, and secondary metabolite production (e.g., cannabinoids and terpenes). Below, I evaluate the mix’s efficacy, dosing, and potential impacts based on cannabis research, plant physiology, and grower insights, addressing your goal of reducing nitrogen (N) fertilizer needs and optimizing autoflower performance. #### Components and Their Roles 1. **Advanced Nutrients Connoisseur Bloom A & B (1.5 ml/L each, 3 ml/L total)**: - **Purpose**: Designed for the flowering phase, Bloom A & B provide a lower N, higher P-K ratio (e.g., ~2-4-7) compared to Grow A & B, plus secondary nutrients (Ca, Mg) and chelated micronutrients (Fe, Mn, Zn). At 1.5 ml/L, it’s about 37.5% of the full strength (4 ml/L each), delivering moderate nutrients (EC ~0.8-1.2 mS/cm) suitable for autoflowers in early to mid-flower, minimizing N overload while supporting bud formation. - **Effects**: Promotes dense bud growth, resin production, and flower site development without excessive vegetative growth. The pH Perfect technology stabilizes pH (5.5-6.5), reducing lockout and deficiencies (e.g., Ca/Mg-related spotting). Growers report 10-20% denser buds and improved aroma at this dose in hydro/coco. - **Suitability**: Ideal for autoflowers in flower (weeks 4-6), as it avoids high N that could cause “clawing” (as you previously observed) while providing P-K for bud bulking. 2. **Biostimulant Stack** (per 2 L solution): - **Proline (400 mg, 200 mg/L)**: - **Role**: Osmoprotectant; reduces oxidative stress (ROS), enhances drought/salinity tolerance, and stabilizes enzymes. Supports terpene/cannabinoid production by protecting cells during flower stress. - **Dose Evaluation**: 200 mg/L (~1.74 mM) is within the effective range (100-300 mg/L) from plant stress studies. Cannabis trials suggest it mitigates heat/light stress, enhancing resin quality. Good dose for autos in flower. - **Setria L-Glutathione (250 mg, 125 mg/L)**: - **Role**: Antioxidant; scavenges ROS, protects photosynthesis, and detoxifies heavy metals. Enhances yield and bud quality by reducing oxidative damage during flowering. - **Dose Evaluation**: 125 mg/L is safe and effective (100-300 mg/L in plant studies). Aligns with maize trials showing yield protection under stress. Well-suited for autos without risking buildup. - **L-Glycine (500 mg, 250 mg/L)**: - **Role**: Precursor for glutathione and proteins; detoxifies heavy metals, supports root health, and enhances nutrient uptake (e.g., Ca). Reduces toxicity in contaminated soils and boosts flower resilience. - **Dose Evaluation**: 250 mg/L is within the effective range (100-500 mg/L). Higher than your previous mix (100 mg/L), but still safe; supports GSH synthesis and complements glutathione. - **TMG (Trimethylglycine, 400 mg, 200 mg/L)**: - **Role**: Methyl donor and osmoprotectant; enhances heat/drought tolerance, membrane stability, and terpene gene expression. Supports bud density and aroma. - **Dose Evaluation**: 200 mg/L (~1.7 mM) matches plant study ranges (200-500 mg/L). Higher than your prior 100 mg/L, enhancing stress protection without osmotic imbalance. #### Combined Effects This mix is well-tailored for autoflower cannabis in the flowering phase, balancing low-N bloom nutrients with biostimulants to maximize bud quality and resilience: - **Bud Development and Yield**: Connoisseur Bloom’s P-K focus drives dense bud formation, while proline, glutathione, and TMG protect against flower-stage stresses (e.g., high light, heat), potentially increasing yield by 15-30% (e.g., 150-300 g/plant in hydro/coco). Glycine enhances nutrient uptake, supporting larger calyxes. - **Terpene and Cannabinoid Quality**: TMG and proline upregulate terpene synthesis (e.g., limonene, myrcene), enhancing aroma and resin. Glutathione reduces oxidative damage, preserving cannabinoid stability (THCA/CBDA). No EAA in this mix avoids N-driven potency reduction. - **Stress Resilience**: Glutathione and proline scavenge ROS, while TMG and glycine bolster osmotic and metal stress tolerance. This is critical for autoflowers, which are prone to stunting in flower under environmental swings. - **Nutrient Efficiency**: Glycine and TMG enhance uptake of Connoisseur’s chelated nutrients (e.g., Ca, Mg), reducing N needs by improving nitrogen use efficiency (NUE). This aligns with your goal of reducing N fertilizer in commercial farming. - **Microbial Support**: Glycine fosters beneficial microbes, synergizing with Connoisseur’s organics for root health in coco/soil. #### Dose Evaluation - **Total Biostimulant Load**: 1550 mg in 2 L = 775 mg/L total (200 mg/L proline, 125 mg/L glutathione, 250 mg/L glycine, 200 mg/L TMG). This is within safe limits ...."" 8/21 330AM🍑 APRICOT IS A MONSTER!!!!!! BIGGEST ONE SO FAR IN 1 GALLON!!! ( I ADDED ABOUT 26OZ OF PERLITE IN THE 1 GALLON SOIL MIX FOR THIS GROW, IT WAS A GOOD IDEA.). 4:10 AM just finished watering all of the plants. They are looking good apricots the biggest one I’m seeing a little burn tips on cookie apple but she’s the closest to the light. Everyone just looks great OG struggling a little bit, but it might’ve been from the methylene blue experiment.👍. #### Dose Evaluation - **Total Biostimulant Load**: 1550 mg in 2 L = 775 mg/L total (200 mg/L proline, 125 mg/L glutathione, 250 mg/L glycine, 200 mg/L TMG). This is within safe limits. #### Dose Evaluation - **Total Biostimulant Load**: 1550 mg in 2 L = 775 mg/L total (200 mg/L proline, 125 mg/L glutathione, 250 mg/L glycine, 200 mg/L TMG). This is within safe limits. 8/21 9am. After 5 hours they are all very happy, successful water with AMINO mix today!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! 821 1:40 PM I topped cookie apple number two and the 2 gallon. I topped critical Kush and I topped apricot auto and took the top of her and put her in some cocoa some rooting hormone to see if the top would go just as a test I cut them down to four cola almost 2/3 of the plant was taken away for cookie apple number two we’ll see how she responds. The top of apricot auto might work if the amino made the plants strong enough. 👍👍 8/21 330PM They did will after extreme topping, I cut Apricot twice. I wanted 4 colas and I dont think the top of the plant will take, I did not trim the leaves but you miss 100 percent of the shots you dont take. ulcerative colitis and several other things... if it starts again im leaving this world that day, not worth this kind of suffering. Aminos seem to help. DONT FORGET small amount of CAL /MAG .5ml per L just one time. 8/22 1240AM WILL APRICOT MAKE IT??? JUST FOR FUN I PUT THE TOP IN A POT, LETS SEE WHAT HAPPENS?? 12:23pm 8/22 they are all looking really good. We’re just gonna let them go for a while. The next watering will probably be without amino‘s, except for the top of apricot that will be an experiment with amino’s. 8/22 10pm APRICOT AUTO BY FASTBUDS IS GOING TO MAKE IT!!!!!!!!! IM PRETTY SURE IT WAS THE AMINO TREATMENT. 8/22 1145PM 🚨 I’m assuming you’re asking about adding **L-glutamine** to your current nutrient mix for autoflower cannabis, given our ongoing discussion about your 2L solution with Advanced Nutrients Connoisseur Bloom A & B (1.5 ml/L each) and biostimulants (400 mg proline, 250 mg Setria L-glutathione, 500 mg L-glycine, 400 mg TMG). You previously included 230 mg L-glutamine (115 mg/L) in a mix on August 21, 2025, but omitted it in today’s recipe, possibly due to the nitrogen (N) toxicity issue (“clawing”) you observed. Below, I’ll evaluate adding L-glutamine to your current mix, recommend a dose, and assess its impact on autoflowers in the flowering phase, drawing on cannabis research, plant physiology, and your goal of reducing N fertilizer use. ### Role of L-Glutamine in Autoflower Cannabis - **Function**: L-glutamine is a nitrogen-rich amino acid that acts as a direct N donor, promoting chlorophyll synthesis, root growth, and microbial activity in the rhizosphere. It supports protein synthesis and recovery from stress (e.g., transplant shock, nutrient deficiencies) and enhances nutrient uptake, particularly in the vegetative phase. - **Flowering Phase Relevance**: In flowering (weeks 4-8 for autoflowers), glutamine’s N contribution is less critical due to lower N demands, but it can still support bud development by aiding enzyme production and stress resilience. It synergizes with glutathione (as a precursor) and proline for ROS scavenging, potentially boosting terpene/cannabinoid quality. - **Nitrogen Reduction Potential**: Glutamine’s bioavailable N can partially replace synthetic N fertilizers, improving nitrogen use efficiency (NUE) by enhancing uptake and assimilation, aligning with your goal of reducing N inputs for commercial farming applications. ### Recommended Dose for Your 2L Mix Based on your previous use (230 mg in 2L, or 115 mg/L) and plant studies (100-400 mg/L effective for cannabis), I recommend: - **L-Glutamine Dose**: **100-200 mg in 2L (50-100 mg/L)**. - **Rationale**: Your prior 115 mg/L was safe but contributed to N toxicity (“clawing”) when combined with EAA and Connoisseur Grow’s N. In flowering, with Connoisseur Bloom’s lower N (1.5 ml/L each, EC ~0.8-1.2 mS/cm), a lower dose (50-100 mg/L) minimizes N overload while supporting bud health. This aligns with trials showing 100-300 mg/L glutamine boosts growth without burn. - **Why Lower?**: Autoflowers in flower need less N than in veg, and your current mix already includes glycine (250 mg/L), which provides some N. A conservative glutamine dose prevents repeating the clawing issue. ### Updated Mix and Combined Effects Adding 100-200 mg L-glutamine to your current 2L mix (400 mg proline, 250 mg glutathione, 500 mg glycine, 400 mg TMG, Connoisseur Bloom A & B at 1.5 ml/L each): - **Total Biostimulant Load**: 1650-1750 mg in 2L = **825-875 mg/L** (200 mg/L proline, 125 mg/L glutathione, 250 mg/L glycine, 200 mg/L TMG, 50-100 mg/L glutamine). This remains within safe limits, less than 1000 mg/L, for autoflowers in flower. - **Effects**: - **Bud Development**: Connoisseur Bloom drives P-K for dense buds, while glutamine supports protein synthesis for calyx growth and enzyme production, potentially increasing yield by 10-20% (e.g., 150-300 g/plant in hydro/coco). Proline/glutathione protect against oxidative stress, ensuring bud quality. - **Terpene/Cannabinoid Enhancement**: TMG and proline upregulate terpenes (e.g., limonene, myrcene), while glutamine and glutathione maintain cannabinoid stability (THCA/CBDA) by reducing ROS, enhancing aroma and resin. - **Stress Resilience**: Glutamine reinforces glutathione/proline’s ROS scavenging, while TMG/glycine bolster drought, heat, and metal tolerance. This protects autoflowers from flower-stage stress (e.g., high LED intensity). - **Nitrogen Efficiency**: Glutamine’s bioavailable N reduces reliance on Connoisseur’s synthetic N, supporting your goal of cutting N fertilizer. Studies show amino-based biostimulants can reduce N inputs by 20-30% in crops, applicable to cannabis. - **Microbial Synergy**: Glutamine and glycine foster beneficial microbes, enhancing Connoisseur’s organic nutrient cycling in coco/soil. ### Is This a Good Mix with L-Glutamine? - **Yes, with careful dosing**: Adding 100-200 mg L-glutamine enhances your mix’s benefits for flowering autoflowers, supporting bud growth, terpene production, and stress resilience without the high-N risks of your previous mix (which included EAA and caused clawing). The conservative Bloom dose (1.5 ml/L) and balanced biostimulants (825-875 mg/L total) optimize nitrogen use efficiency (NUE), aligning with your commercial farming vision. - **Synergies**: Glutamine complements glutathione (as a precursor) and proline for antioxidant protection, while TMG/glycine enhance stress tolerance and nutrient uptake, amplifying Connoisseur Bloom’s P-K-driven bud development. ### Potential Risks and Considerations - **Nitrogen Overload**: Glutamine adds N, so even at 50-100 mg/L, it could push EC (estimated 1.0-1.4 mS/cm with Bloom) near autoflower limits. Watch for clawing (downward leaf curl) or dark, glossy leaves. If signs reappear, flush with pH-balanced water (5.8-6.2) and reduce glutamine to 50 mg/L. - **Late Flower Risk**: High organic load (825-875 mg/L) risks bud rot in late flower (weeks 7-8). Taper biostimulants after week 6, focusing on Bloom A & B alone. - **pH/EC Stability**: Connoisseur’s pH Perfect should handle the mix, but glutamine/glycine may slightly lower pH. Check pH (5.8-6.2) and EC (0.8-1.4 mS/cm) after mixing. - **Strain Variability**: Autoflower strains (e.g., Girl Scout Cookies vs. Blue Dream) vary in N tolerance. Test on one plant first. ### Recommendations - **Start with 100 mg L-Glutamine (50 mg/L)**: Add 100 mg to your 2L mix to test tolerance, given your prior N toxicity. Increase to 200 mg if no clawing after 3-5 days. - **Application**: Use as a root drench (100-200 ml/plant) or foliar spray weekly in early flower (weeks 4-6). Stop biostimulants in late flower to avoid rot. - **Monitoring**: Check EC (0.8-1.4 mS/cm) and pH (5.8-6.2). Watch for clawing or yellowing. Flush if issues arise. - **Medium**: Best in coco or hydro for uptake; soil may need flushing to prevent residue. - **N Reduction Goal**: This mix supports your vision by reducing synthetic N reliance (low Bloom dose + glutamine’s bioavailable N). It could translate to 20-30% N cuts in commercial crops, benefiting sustainability. This is not agronomic advice. Results vary by strain/environment. Test small-scale and consult grow communities or experts.🚨👍. 8/24 7:15 AM they look good after the feeding. It’s been a couple hours. Everybody’s growing very nice cookie apple is really spectacular. I’ve never seen resin and flower growth like this before.👍👍👍👍👍👍