This week I started low stress training on all three plants to try and help grow an even canopy over the next few weeks. 🌱🌱🌱 More possible deficiencies showing on the lower fan leafs but all three plants look for the most part healthy and are still showing good growth each day developing more and more bud sites . 🙂✌️

Flower Week 2

Its week 11, Harvest time!! .... or is it? Well, i can tell you - absolutely not. 💩 #3 is well into flower right now, but i dont think she's gonna come down for another 3 weeks. She's def. the furthest along though. I'll be using the thrichoscope end of this week i reckon. #2 and #1 are both just barely getting started in their flower, so they will probably run for another 5 or 6 weeks at least. i've got good hopes for plants 2 and 3, and also feel like the light-intensity experiment is really giving me some solid results. (the one with the least light is #3, whhich even started to flower much sooner than the other two, i feel like there is a link with the scarcity of light energy it receives. anyone here that would confirm or deny such an idea?)

FOR UNKNOWN REASONS I'M MUTED SINCE MORE 72 HOURS, THANKS FOR SUPORT MY FRIENDS, I CAN'T REPLY YOU ON COMS AND DM, BUT THANKS FOR LIKES AND COMS! Hello my friends 👩‍🌾👨‍🌾, This 2 weeks vacation and remote growing was nice, Except 2 points - light was 35% so the plants stretch up too much this week - I've founded perforated leaves on the #2, I don't know If it's leaves problem or bugs, I'll check tomorrow with my Microscope . The bottles was enough for a week with litle plants, with biggest plants must to use an other way. I've put my lamp @50% and plants close around 35 cm. Next week I'll work a bit on the plant, topping, training, to prepare my scrog🌲🌲 And return to manual watering. The soil look a bit too much watered, I'll stop watering for a couple of days. Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Also to @marshydrococo2 , @News_SweetSeeds for sponsoring 💕💕. Mars Hydro TS 1000 https://www.mars-hydro.com/ts-1000-led-grow-light Gorilla Girl F1 fast version https://sweetseeds.es/en/photoperiod-dependent-seeds/3065-gorilla-girl-f1-fast-version.html See you next week my friends Have a good week end 😁💕

Almost tried everything by now but the roots wont take any nutrients, so i started using Ripen from ghe and i will prob start using R/o water with just calmag after this water. Gonne run this prob for 10/11 weeks since i slowed them down badly. trichomes are mostly clear but gotta see how long she can survive haha They are sticky as fuck and the aroma's are the best i ever had,so i will be starting a new round this week!! Cya next week!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

4/6/2024 - Vegetation Week 8 Day 1- Last week in VEG, They are all looking amazing and no worry about stretch when I flip Next Saturday they are indica and I have more than 50% of distance from the top of the plant to the lights with a little more I can raise the lights if necessary. So going to really just enjoy this last week of VEG, and get my Cloning equipment ready. I will be taking 2 cuts for each this is also a PHENO hunt for HWG and he not only has the prize for the winner but he also has an additional bounty on a cut of the winner. I did have to take some from around the edges to keep them from touching. 4/7/2024 - Vegetation Week 8 Day 2- Didn't do anything today but watch them grow.. They will be getting a really good flip trim and clean up for clone material on Friday/ Saturday. 4/8/2024 - Vegetation Week 8 Day 3- Couldn't even get a picture of the #1 at first too much Foliage throughout.. So I had to clean that up and make sure they all still have some separation before flip. 4/9/2024 - Vegetation Week 8 Day 4- Huston we almost had an issue.. I looked at my Res and she was in desperate need of 10 gallons to get her through until next water change on Saturday. So I added 10 gallons and the Nutes. I added 10 Gallons of Water I added the following Nutes: CalMag= 1.00Mil/Gal= 10Mil FloraMicro = 5.4Mil/Gal= 54Mil FloraGro= 4.2Mil/Gal= 42Mil FLoraBloom= 4.6Mil/Gal= 46Mil 4/10/2024 - Vegetation Week 8 Day 5- Had to giver them a huge clean up today I couldn't even see the numbers in the first pic. I am also worried that #1 and #3 really, really are getting huge over the last few days.. starting to think I should have flipped them this past Saturday.. The interesting things is even though this is an Indica Strain, I might have to super crop just to keep them off the lights. The joys and sorrows of RDWC and timing. 4/11/2024 - Vegetation Week 8 Day 6- Even though Flipping in Two days almost wish I would have gone at Week 7. I am really worried about #3, #1 and #2 are huge and when I put them in flower they are going to stretch out 4/12/2024 - Vegetation Week 8 Day 7- Day of Work.. Lots of Work.. I gave all three a Cloning/Flower cleaning.. all three got cleaned up top Inside and bottom, I took 2 cuts of each to see which one come out on top then I will kill off the other cuts once I have a winner in flower. I clean up this thoroughly before I flip because it reduces the work load on the next major clean up I do on Week 4 of Flower.and they are so much taller that it is going to be a pain in the ass to maintain canopy space for #3, we will see how it goes and adjust as necessary..

23.09.23-85 дней этому растению . Она немного растянулась и я решил убрать пару больших листьев и немного подвязать , чтобы свет лучше проникал . На этой неделе я поднял ппм раствора до 1000 . Все девочки активно пьют и выглядят здоровыми . 26.09.23-88 день ) За эти 3 дня растение продолжило растяжку и прибавило около 8 см 😎😎 Шишки красиво формируются и на листьях уже виден Сахар . В планах на ближайшие дни провести легкую дефолиацию и добавить света . Всем мир 28.09.23- 60 дней вегетации и 30 дней цветения ! Растение продолжает медленную растяжку и прибавило 5 см за 2 дня , в остальном изменений нет . Завтра добавлю ей 120 w .

01/26/2021 She's a 9 week strain, hopefully puts on some more weight, one bud is flopping over, took _ liter of water

Wow, she's getting pretty stinky. Very earthy, dank notes. Trichomes are popping up all over and the buds are setting up well. Did some hst on a few branches to see what difference it makes. The stems on those 2 branches have already formed the typical knots. I'm anxious to see if it really impacts yield when compared with similar branches from the same plant.

she germinated on November 18, 2017 outdoor .... it has 168 days ... it has 24 weeks ... and as always a strong smell ... to indica... and IWill cut it on Sunday...its 170 days. outdoor .... I cut.a branch 1 month ago and it was very good the smelll.the taste... patience almost comes to an end 2 more days ....

Frosty !! And Dark ! Going to def regrow under stronger lights

👇 This week: --- Watering 1l every day. Some phenos need more PPFD at canopy height approximately 800-850 (Increase slowly), VPD ~1.2 Big defoliation, stretch coming to the end Orange Creampop #2 HLVd test came negative Next week: Lollipopping, compost tea, defoliation --- Happy growing and thanks for checking out my report! I really appreciate you! 😁💪🙏

Potted up a couple put the lights bak on tent up n running @ it as per usual noticed some wonky ass long leggy seedlings 🌱 and the monster cropped clones hav started to a dunno kind of dry out and cannabslise maybe due to stress as it had started budding again am goin for couple of weeks 24 hour light cycle then goni drop to 20 on 4 off then 18 6 just before a flip to flower a just want to try this out while am vegging also hav a blueberry autoflower in the tent al soon be fighting for space a know that but a like to push the boundaries lol all the best to all my fellow growers out there stay tuned it’s early days yet but a can see things getting messy @chedderbob112 @s2340420

Miei cari amici, L'estate incalza e finalmente il tempoi permette di trasformare ad hoc. Secondo i gusti il giardino, la terrazza e i due balconi. Un lavoro duro e intenso con i 25/30 gradi che il NW in questa settima sta proponendo e che spero vivamente durino tanto. Travaso storico e professionale. Vediamo ciò che l'armadio dice. Questo travaso probabilmente ritardera di due o tre settimane il raccolto, ma andava fatto e ne varrà sicuramente la pena. 28/06 My dearest friends, comrades and farm partners. Doc. Cannas is extremely happy to introduce you to the 5 wonders of Expert Seeds. Still my most heartfelt congratulations for their genetics, between photos and videos you have seen what a wonder these little girls are growing. Today they have been introduced to LST, an art halfway between Astana yoga and BSDM. Being their first time there was a need for love and passion. So it was. At the next update farmers

Start of week 2 flower - days 22 to 28 October Light is at 90w and it will remain on the top of the tent for the entire grow. -------------------------------------------------------------------- 22/10/2020 - Transplanted #1 to a 2 gal pot, shes s bit sad due to over-watering. 23/10/2020 - Little hard bend on #2 applied quickly zip tied it. 27/10/2020 - Watered #2 added only Cal-Mag, runoff about 850 ppms. 26/10/2020 - Increased the light to 100w. 28/10/2020 - Watered #1 with Cal-Mag and Great White.

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Today the week is not complete, but I decided to write a report. Today I switched the plants to flowering, this is a significant day, from which we will start counting the weeks of flowering. But over the past 4 days, I will show you a timelapse of my wild animals, and share the problems)) Let's start sharing: 1. Both of my bushes seem to have been overexposed to veg, they are very large for my growbox. The problem is that I was greedy and wanted the right bush to catch up with the left, but this did not happen. 2. The left bush started to show a lack of P&K, I attribute it entirely to the supply of co2, my fertilizers are not designed for the supply of co2. I added the missing chemicals to the irrigation system, and everything seemed to be fixed. 3. After installing the gas supply system, I added co2 24 hours a day. I consider this a mistake. Plants use CO2 during the day and oxygen at night. Now I have corrected this and at night the plants do not receive CO2. Now the night level of CO2 is 400, the daytime level is 1000 ppm. PS. The last photo in this week's review will relate to the question of what the plant is missing! Looking forward to your feedback and tips! Sincerely, Your Five