Dopo 6 settimane di vegetativa per le nostre piccole talee, ormai velle grandi, arriva il momento in andare in fioritura e continuare a crescere e fiorire!!. Seguiranno aggiornamenti. Martedì 20/02/2024 Inizio controllo 2027 ec 5.5 ph Cambiamo soluzione e iniziamo con i nuovo dosaggi da prefioritura Aggiungiamo 75 lt demineralizzata Base 285 ec 7 ph Aggiungiamo i nutrienti per 80 lt 160 grow 160 micro 120 bloom 90 sensizym 90 calmag 90 rhino skin 90 bud candy Otteniamo 2146 ec 6.3 ph Aumentino al 100% la nostra mars hydro fc6500 e la teniamo a 40 cm di distanza.

18. November Die sechste Woche ist angebrochen. Heute keine Einsicht unternommen. 19. November Die Begleiter kommt so langsam aus der Erde. Sind noch wenige. Spätestens in einer Woche neu nachlegen, falls nicht ausreichend wachsen. Die drei Damen entwickeln sich prächtig. Stresssymptome durch das schneiden sind kaum noch bemerkbar. Cookies gelato kommt mit den neuen Hauptrieben sehr gut zurecht. Wachstumstempo hat deutlich zugenommen. Tropimango sieht deutlich gesünder aus. Auch bei ihr ist endlich mal etwas Tempo im Wachstum spürbar. Die Runtz fühlt sich aktuell so an als würde sie den Rahmen des Zeltes sprengen wollen, sobald es in den blütestretch geht. Die Triebe haben sehr dicke Stämme, die Blätter sind verhältnismäßig zu den anderen Pflanzen sehr groß. Selbst neu entwickelte Triebe und Blätter starten deutlich größer als ich es bisher gewohnt bin. Aber schaun wa mal was wird… was wird. Der Fimmingschnitt hat mir einen schrägen Trieb hervorgebracht. Ka was ich damit machen soll. Nächstes Mal halt gerade schneiden 🤝 20. November Runtz hat ein unglaubliches Wachstumsbedürfnis. Ihre Blätter sind riesig und tiefgrün. Sie verdeckt innerhalb von Stunden erneut ihre anderen Triebe Sie trinkt auch deutlich mehr als die Cookie & Tropi. Hab ihr heute nochmal 3l Wasser mit dem vollem Düngerprogramm zugemutet. Die anderen beiden kommen gut voran, haben allerdings noch kein Wasser bekommen. Die Töpfe sind noch zu schwer. Tropimango wuchert in alle Richtungen Cookies Gelato darf weiterhin an Höhe gewinnen. Ich spiele mit dem Gedanken sie kurz vor dem Wechsel in die Blüte erneut zu toppen. 😅 21. November Die Cookies gelato wurde mit 3l Wasser versorgt. Zusätzlich gabs noch 1ml power buds von plagron. Kleine Vorbereitung auf die Blüte. Tropimango hat 2l Wasser mit den normalen düngeschema erhalten. Runtz wurde gestern mit 3l versorgt, ebenfalls normale düngeschema. Zusätzlich hab ich ihr die großen Blätter genommen, sowie die seitentriebe neu ausgerichtet 22. November Habe bei der Runtz den schiefen Trieb abgeschnitten. Er ist leider viel zu verstümmelt. Die Idee war dass dieser Trieb dementsprechend der Pflanze Zuviel Energie zieht, die sie an einer anderen Stelle besser investieren könnte. Daher im heute der Schnitt Cookies Gelato gewinnt an Höhe. Im Verhältnis zur Runtz ist sie unglaublich gut ausgeprägt, ist allerdings auch 14 Tage älter. Die Tropimango sieht weiterhin merkwürdig aus. Ich kann leider nicht sagen ob ich beim Erde mischen irgendwas falsch gemacht habe. Weiß auch nicht wie ich drauf Einfluss nehmen könnte. Aber sie wächst. Von daher geht’s einfach weiter. 23. November Die Woche neigt sich so langsam dem Ende zu. Nach ewigem umentwcheiden kam ich jetzt dennoch zu dem Entschluss, die Pflanzen zu Beginn der neuen Woche in die Blüte zu schicken. Die Cookies gelato hat daher noch ihr letztes topping bekommen. Nachdem sie einen ordentlichen Wachstumsschub bekommen hat, gehe ich von aus dass sie es gut wegsteckt. Die tropimango gefällt mir immer besser :) Ich hoffe sie geht in der Blüte nicht unter, da sie eine babygenetik ist. Runtz (irgendwie hört sich der Name eklig an. Muss da an einen alten Mann denken, der seine Nase hochzieht und dann auf den Boden spuckt) wird so wie sie wird. Der Stamm ist super stabil aber sie ist verhältnismäßig klein für ihr Alter. Dafür produziert sie immer noch riesige Blätter. Cookies gelato ist nun 39 Tage alt. Tropimango ist nun 36 Tage alt. Runtz ist nun 25 Tage alt.

SUNDAY 5/5: Rearranged the gardens..have 17 plants in the 4 x 5 now...yowsa! MONDAY: Plucked some dead leaves and did a trichrome check on her. She's getting close now! Prolly about 80% cloudy and 5% amber! I'll start flushing her tomorrow or the next day if she's not dried out yet. TUESDAY: I observed some white spots on a few leaves here and there, so I sprayed her today with Trifecta Crop Cure, a concoction of a bunch of natural plant oils. I already decreased the humidity in the room to 45% with my new 70L dehumidifier, so I think she'll be fine. I'll spray her again tomorrow and the next day, then I'll have to hope that did the trick, because she is already in the harvest window. WEDNESDAY: I flushed her today with about 4 gallons of ph'd spring water and bembe. It was her last dose of any nutes...flushing with only spring water from here on out. THURSDAY: Took a couple of photos and tried installing my new Solacure FlowerPower UVB light, but it was dead on arrival.😢..back to the 15.0 reptile UVB lights for now I guess...

Week 4 and she is in the last week of training as we can see all the side colas coming out nicely. She has taken 2 lots of feed no problem and is now flowering. I think this is going to be a good plant.

Day 47 12/12 3 plants in 2x2 no more nutes will be fed from now just ph balanced water for the next 2 to 3 weeks until harvest, looking forward to trying this stuff smells so good and looking very frosty with nice colours starting to show I’m dropping my night temps down to 15 degrees Celsius to hopefully bring out some more nice colour, can anyone guess the final weight 🍁

Day42 of flowering Girl #1 seems to have tighter buds and is still smaller, but looks like she will finish later (although she popped first by a few days) Girl#2 is much heavier and has sucked the green from her lowest fan leaves even though she is fed well. She also smells soooo good, over ripe cantaloupe/melons. I am really happy with these plants, which I did not top and let colas grow. I am thinking of removing the cola fan leaves to let light blast the other tops. Day 45, Removed almost all fan leaves to expose as much bud as possible to lighting. Scent is very strong and sweet. Very resinous leaf

This lady has picked up alot this week, going to maybe start trickling nutes next week and see how she takes off, happy growing and stay green ✌️ 💚 👌

Still feeding, just letting them do their thing in their small pots. They are doing really well and showing no issues.

Not much growin on this week just watering and letting them grow. No smell yet Increasing light to 80% Nutrients in previous week and next week

Veg22, riego con 3.0 EC y pH 5.9, drenaje promedio de 5.8 pH y 2.6 EC(muy bien), primer riego con Sensizym para limpiar un poco las raíces, y este será el último riego de vegetación con Voodoo Juice, Tarantula y Piranha. Se hizo una pequeña defoliación de las hojas abanico más bajas y se volvió a ajustar el LST, ya van tomando la forma final. Veg23, creciendo bien. Veg24, se mide la temperatura de las hojas 22°C y se compara con la temperatura del indoor 23°C, con una humedad de 50% RH, la calculadora arroja un valor de VPD=1.24 kpa, lo que está dentro del rango óptimo. Veg25, hoy toca una pequeña defoliación y probaré también hacer un último apical en 2 o 3 plantas a modo de prueba. Al final, tomé un gran riesgo, y hice varios apicales extra y una defoliación más grande de lo que tenía en mente, tengo fe que resultará bien 😎👌 Veg27, riego con 0.8 EC y pH 6.0, drenaje de 6.0 pH y EC 2.0, se están alimentando y creciendo bien, quedan casi 2 semanas para pasar el fotoperiodo a 12/12, espero que crezcan suficiente para esa fecha. Veg28, un par de hojas de 2 plantas presentan mordidas tipo oruga, revise y no encontré nada, aplique spray insecticida, espero no siga pasando. A parte de eso siguen creciendo bien, quedan un poco más de 10 días para el paso a 12/12.

It’s been a couple weeks since my last post. I’m currently in the middle of the first week of flushing. She looks like she’ll be ok.

En general me encanto cultivar esta genética, siempre fue la primer genética que quise cultivar desde que empecé en esto y no me decepciono en lo absoluto, de una sola semilla obtuve 106 gramos de flores de gran calidad 😃 increíble para ser mi primer cosecha con semillas de bancos, ya quiero ver que resulta en la próxima cosecha ya con más experiencia y mejor equipo 🤩

These plants are starting to blow up. 💣 These are some very healthy looking plants but what else can be expected from such stable genes.🤷‍♂️🏽 just removed some lower most branches, everything below where o first topped really. ✂️ just a few more weeks before the flip to flower.🌺

Le 42e jour ce passe très bien. Les plantes sont au milieu de la prefloraison et ne vont pas tarder à arrêter leurs croissance pour se concentrer sur les bourgeons ainsi j'adapte l'alimentation au circonstances. J'ai rattrapé l'erreur de pH même si cela reste précaire les plantes se portent dans l'ensemble très bien 🌱🙏

I might switch the lights in the next days. Depending on her growth. Day44: Added some water and moved the camera a little further away. Day45: She felt lonely, so I got her a friend... Theobroma Cacao. New seedling that needs the warmth + humidity. Hopefully surviving winter. Day46: I decided to take some zoomed in stills of her. To show off her beauty. The purpling of the branches really make this lady something special. In some of the shots it might look like her leaves are yellowing, but it's just the angle of the light. You can see on the photo that I took from the top, that all leaves are a healthy dark green. At least I now have proof that she's a lady. She's short and stubby, but I'm not really growing for amount. I just neeed 50g, after all (hehe) Day47: She grew like a demon overnight. I'll check the height tomorrow.

The plants are growing at a good rate now and drinking a litre each per day. I feed them by hand and each plant gets 2litres every other day. Didn't do anything else this week other than install the trellis on the last day. Love the way these genetics grow and fill out the tent. When I was growing the mother in the pheno hunt I remember remarking how well this plant took to being topped. These 2 plants are front and right in the timelapse video.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

! this is my current status, gonna upload my last veg weeks within the next week - got a bit busy around Spannabis - thank you for your understanding! 💚 Welcome to Bud Boutique Grow Diary - really appreciate all your love and support :) Dont forget to check out my other current grows! 🗓️ This Week: - Day 24: attaching once a week APTUS Foliar with Regulator & Nutrispray with the amazing CannaFogger by Petra Grow - Day 28: bud development is super beautiful and praying up Thank you for still staying with me 💚 ___________________________________________ --- 🌱 Strain (Sponsor) --- 🏷️ Stardawg by MSNL https://www.marijuana-seeds.nl/stardawg-feminized-seeds --- 🥗 Nutrients and Feeding (sponsored by APTUS: APTUS Ambassador) --- 🍸 APTUS: full nutrient schedule extreme -- Regulator, N-Boost, P-Boost, CaMg-Boost, K-Boost, Allin1 Liquid, Startbooster, Topbooster, Enzym+ every feeding -- Fulvic-Blast, NutriSpray as Foliar each once a week 🔗 https://aptus-holland.com/ --- ♻️ Grow Control (Sponsor) --- TROLMASTER: TENT-X + LM14 Light Adapter to dim/sunrise/sunset lights + Temp & rH Sensor all remote on App 🔗 https://www.trolmaster.eu/ --- 🚿 PetraGrow (Sponsor) --- CannaFogger Foliar Spray 🔗 https://www.petratools.com/product/petragrow-cannafogger-atomizer-new-mini-fogger --- 🏭 Grow Setup --- 💡LUMATEK Zeus Pro 600 * 🏠🌿 Indoor: Homebox 120x120x200cm (4x4) * 📐🌀 PrimaKlima exhausting Fan 1180m3/h (running on 60-80%) * 🌀 Can Light Filter 800m3/h & 1x Fanbox 1x Dyson fan for Air circulation 🔗 https://lumatek-lighting.com/zeus-600w-pro-29/ 🔗 https://primaklima.com/de/shop/ventilatoren-de/ec-ventilatoren/pk160ec-tc/ 🔗 https://canfilters.com/products/filters/ All Likes and comments are highly appreciated!!! 👨‍🌾 don't forget to check out my Instagram for daily educational content: budboutiquee - Bud Boutique