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Vamos familia, actualizamos la sexta semana de crecimiento de estas Terp & Tonic de Seedstockers. La temperatura que estuvo entre los 24-26 grados y humedad dentro de los rangos correctos. Esta semana ya cambiamos a 12 horas luz,12 horas de oscuridad, estiraron bien y ensancharon bastante también, el color es verde sano. Se nota que los nutrientes de la marca Agrobeta hacen sus funciones. También realicé una poda de bajos que yo si las veo necesarias para explotar después la flor. - os dejo por aquí un CÓDIGO: Eldruida ,descuento para la tienda de MARS HYDRO. https://www.mars-hydro.com Hasta aquí todo, Buenos humos 💨💨💨
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La planta sigue ramificando e inicia la pre floración, se prepara para dar el último estiron. Mientras tanto yo le cambié el fotoperiodo a 20/4 y le agregué un foco de sodio de 400w a los 200w de cfl que ya tenía en vegetación. Volví a ajustar el lst creo que por última vez antes de dejarlas ser hasta el final. Riego cada 4 días aproximadamente con 2L de agua estacionada con ph entre 6.2 y 6.4. Ayer le tocó agua pura y en cuanto me pida más le daré un poquito de extracto de algas de top crop. Big one y top auto en muy bajas dosis, pues la planta no ha mostrado signos de deficiencia alguna.. Hice un riego foliar con aceite de Neem, extracto de canela y jabón potásico a modo preventivo. Ha respondido bien a la nueva luz, la distancia entre el sodio y la copa es de 60cm, iré acortando más adelante 10cm más si la temperatura me lo permite, La sigo!
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@mheat
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She is so tall...cant raise my cob anymore! Lots of sites...smells stanky!! In a good way...sort of.
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She's responding super good to lst method she looks absolutely gorgeous I would have loved to be able to grow her since march however It was not posible but I keep this wonderful indica in my list. This wonderful pheno of Alien gorilla has started flower the 3rd of August.
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8/14 Everything looked beautiful this morning. Seems like I've got the watering schedule down better. I do have one gmo that looks hungry so I need to increase nutes. At least on that plant. Toasted toffy has little spits that could be water marks, pests or septoria. I don't think it's septoria. In the pastvi lost far more leaves when I had septoria. I've been crazy busy. I'm surprised things are going this well. I need to put up the final supports so I can add to them once I've got big heavy buds. I was really stoked to see everything doing so well. The plant in the 50gallon is the furthest in flower. I'm so grateful to have the ability to grow high quality cannabis for myself amd my wife. There's nothing quite like it. That hash I made turned out great. I think I'll press it today. Supports will be done this week. Saw a few aphids on the plant I cut down. Earwigs aren't here this summer or there would be NO aphids. Plus when I cleaned out the insides this morning I found some dead leaves WAY in the middle by the stalk. They would've eaten that shit if they were here. Birds and other predators seem to be keeping pillars at bay. I'm not seeing the damage and when I do sfind one it's absolutely tiny like a newborn. I'll think about whether I need to spray BT. 8/15 That fucking cancel button always fucks everything out. I was done no I need to start over. Didn't water last night. Found pm on my gmo in the 30. Interior of the plant. Should've defoliated better and earlier. Oh well. I'll treat with k bicarb or plant doctor. I'll have to think about it. Other plants are looking good. Well they all are. I just hate pm. I can't isolate it due to local laws so it is what it is. Nothing I haven't dealt with before. Watered today. The middle gmo in the 30 got 1.5 gallons. The other plants each got a gallon. Including the one in the 10gal and 50 gal. Its supposed to rain but only like .1. I also chose to feed today. All plants got a gallon. I DID NOT WATER THE EVENT HORIZON IN THE MIDDLE AND THE SHERB PIE AS THEY WERE PRAYING AND HEAVY. I need to get these supports up. I'll update as I go. Oh and one thing I remembered is that, I didn't properly sanitize the trellis nets I installed on the plant that has pm (non visible today but still). I harvested the plant that used that bet last year was harvested in two stages. The bottom I let go way later and ended up with a little pm on the stuff I left for hash material and it got some pm. I wonder if that's whete this came from. 8/16 Death in the family this morning. I couldn't decide if I should use organocide plant doctor or not. It's a systemic It's been discontinued and reformulated. It's a systemic fungicide. Instead I mixed two tsp potassium bicarbonate with a little dawn in a half gallon mister. I used gloves and defoliated everything that had pm on it. I checked the plants next to it and luckily it hasn't spread yet....but it will. I feel safer using the k bicarb. I'll do more research and try to find the best way to tackle this. I don't want to get rid of the plant and due to local ordinance I can't isolate it. If I can keep it to this one plantvthat would be fine. I'd just use it for extracts. So glad I didn't use all that netting and put up tjosr supports. I'll need to disinfect them if I decide to use them. We'll see. Plants are coming right along flowering. WENT BACK OVER AND DEFOLIATED A BUNCH OF fan leaves and interior stuff to promote airflow. Still saw pm on that plant u had treated earlier with k bicarb. I can't isolate due to laws and I'm not willing to discard this massive monster cropped plant. So I'm going to treat it. I started by removing everything infected and improving airflow on that plant and all the others. I didn't make it to one event horizon. I plan to treat the infected plant with Organocide Plant Doctor since its what I have on hand and I've had good luck. It's a systemic. After I treat the one plant and see that it didn't hurt the plant I'll use a preventative dosage on the other plants. I've battled wpm several times. This is something I'm very familiar with. What sucks is it's totally my fault it happened due to grower error. This has set back me puttingvup my supports as well. Plants look good woth a haircut. Also the toasted toffy had some leaves removed with spots that looked like septoria. I think nutes havecreally kicked things in gear. Now there are little buds on plants. 8/17 BAGS still seemed heavy so I didn't water. It's been MUCH cooler. It's 63 at 9am. It would normally be 80 by now so maybe they aren't using as much water. I watered the the toasted toffy I missed last round but the Sherb Pie still had weight to it. I also watered the GMO on the far side and the one in the 30 as the seemed a little lighter. I looked in and I couldn't find a SPOT of powder mildew on the plant! I know it will come back but on this 100% rh day there isn't a spot I can find! I'm going to go check on them later today. Do some more defoliation and treat the plants with Plant Doctor to try to mitigate the spread of the P.M. Very suprised the k bicarb worked like that. WENT BACK OVER AROUND 11. I WATERED THE TWO THAT DIDNT GET WATERED LAST TIME. THE EVENT HORIZON AND THE SHERB PIE. IT WAS COLD AND OVERCAST. TEMPS HAVE BEEN MUCH COOLER. SOON AS I WATERED THE SUN CAME OHT AND THE TEMP WENT UP. I WENT THROUGH THE PLANTS I MISSED AND SEFOLIATED LEAVES AND INTERIOR BRANCHES TO INCREASE AIRFLOW. SURPRISED TO STILL SEE NO PM WITH 100% HUMIDITY. I WENT THROUGH EVERY PLANT. ILL NEED TO GO TJROUGH AGAIN BUT TJIS IS MUCH BETTER. THE TOASTED TOFFY THAT MAY HAVE SEPTORIA I WILL TREAT TONOGHT WITH PLANT DOCTOR. I REMOVED ANYTHING LOOKING INFECTED. I LSTed THE BIGGEST BRANCHES WITH CLIPS TO THE BAG AMD TWINE. SO AIRFLOW IS MUCH BETTER. I THEN REMOVED A BU CH OF THE MIDDLE. I COULD TAKE MORE BUT ON OUTDOOR HARVESTS THAT LITTLE STUFF GOES IN EXTRACTS. I PUT A FEW HOURS IN TODAY. IM GOING BACK AND ILL TEST THE PLANT DOCTOR ON THE TOASTED TOFFY. BUT IF THE K BICARB WORKS THIS GOOD ILL JUST KEEP USING THAT. 8/18 It started sprinkling when I left this morning. I did more defoliation on a few different plants. Including the healthiest GMO. It seems like things should be further in flower but it is what it is. I'm not doing clones again. Only reason I did is because I lost my 72 seedlings and depleted my seed supply. I hate treating pm. Lost Coast Plant Therapy I'd really whete its at when it comes to treating pm. I might just order that. My commercial buddy told me that he wouldn't use the plant doctor and not to "spray shot all over my plants". He's probably right. I see something small and try to overcurrent. I dont see any more septoria looking leaves on the toasted toffy since I lsted it and removed damaged leaves. I was going to use the fungicide plant doctor on that and then use it ad a preventative. He's probably right. I've put a lot of work in defoliation and such. It WILL spread but it hasn't yet. I removed what I saw. I was going to hit it again with k bicarb but it said it should be weekly treatments.i hope I'm doing things right. I shouldn't be this worried about pm but I've got am anxiety disorder. I'll fully sterilize (AGAIN) my posts and trellis netting before I instal it. I'll probably go check on them later. It's hard to avoid pm with 100% humidity and 30° temp swings. I've got a few lights so maybe this winter I'll do indoor. I'll try to keep this updated. LOOKING BACK AT PRIOR DIARIES I GUESS IM RIGHT WHERE I SHOULD BE FLOWERING WISE. I JUST HAD A COUPLE REALLY EARLY PHENOS A COUPLE TIMES. 8/19 Defoliated some more stuff amd tried to improve airflow. Humidity is high and it was sprinkling earlier. I think we'll have showers. Not seeing much pm. MAYBE a little in the middle of that middle gmo but it could just be residue. I'll treat it again with potassium bicarbonate soon. If that doesn't work I'll switch up treatments. I've got citric acid amd some other stuff too. I almost ordered lost coast last night but decided I'd wait to try it out. The toasted toffy has SOME spots that look like septoria. It's the second furthest in flower and very indica leaning. It seems to be flowering vigorously so I don't want to fuck with it too much. I could use plant doctor on it and see what happens but I'm going to monitor for a few days after defoliating. WENT BACK OVER AROUND 4 AND SHOOK OFFCTHE PLANTS. HADNT RAINED MUCH. BAGS ARE STILL HEAVY. THE TEN WAS LIGHT AND THE EVENT HORIZON BESIDE IT SEEMED A LITTLE LGHT AS WELL. I THINK IT MIGHT BE ME COMPARING THEM TO OTHER PLANTS THAT ARE SATURATED. SEEING HOW THEY BOTH LOOKED THE BEST IVE EVER SEEN THEM I HELD OFF WATERING. ITS ALSO SPRINKL9NG AMD GOING TO RAIN TONIGHT. ILL REASSESS IN THE MORNING. PLANNED ON USING PLANT DOCTOR ON TOASTED TOFFY BUT DECIDED AGAINST IT PARTIALLY DUE TO THE RAIN. PARTIALLY BECAUSE I PUSSED OUT. I WANTED TO FEED. IVE NOTICED SOME FADE AND PLANTS PUSHING AND TRYING TO GET OVER THE FENXE INTO FLOWER. THE TOASTED TOFFY AND THE UNKNOWN IN THE 50 ATE THE FURTHEST ALONG AND DEVELOPING ROCK HARD BUDS. GMO HAS A MASSIVE STRETCH. THINGS CHANGE DAILY. I WANTED TO GET A GOOD DOSE OF NUTES IN SO AFTER THIS LITTLE BIT OF RAIN WHEN WE GET THAT NICE SUNSHINE MY PLANTS WILL TAKE OFF!! THEY'VE BEEN PROGRESSING FAST DESPITE THE SHITTY WEATHER. 8/20 It's still sprinkling. It SAYS WE got zero rain yesterday but that's just not true. Today is supposed to be light showers with like .02 in 9f rain. I mixed up some water to check the plants. I figured a few would he light. The event horizon on the back SEEMED a little light. So did the one invthe ten. I realized it was just comparing it to the bags that were saturated. Still I ended up giving the event horizon in the back a half gallon and split the other half with the one in the 10 that dries out super fast. It's crazy. The weather just abruptly changed one day and I go from watering twice a day to hardly at all! Everything but the sherb pie and the seedling in the 10gal are vigorously flowering. Upping the nutes was a smart move. The seemed to like it. I'm gonna check later and as soon as I can I'll hit that toasted toffy with plant doctor. That's tied for furthest along in flower. I've done a bunch of research and I think this is the right move. I'll keep this updated. After this small patch of shitty weather we are goingvto get some sun amd these girls will EXPLODE! WENT BACK OVER A FEW TIMES. LAST AT SIX. I DID SOME DEFOLIATION AND PRUNING OF PLANT INTERIORS. ITS ABOUT TIME TO TREAT THE TOASTED TOFFY AGAIN WITH PLANT DOCTOR. I CAN SEE SOME SEPTORIA SPOTS. I TREATED THE EVENT HORIZON THIS MORNING. IT GOT A FEW HOURS BEFORE A LITTPE RAIN WND THEN SUNNY AND 80. I USED BETWEEN A QUARTER AND HALF GALLON ON THE INE PLANT. I MAY NOT HAVE USED ENOUGH OF THE MIXED UP SOLUTION ONVTHE TOASTED TOFFY. IT HAD BEEN WORKING AWESOME AND NOW THAT ITS TIME TO REAPPLY I NOTICE A FEW LEAVES THAT LOOK INFECTED. OVER THE NEXT WEEK ILL BE MONITORING THE RESPONS FROM THE PLANT DOCTOR. I'M CONSIDERING APPLYING PLANT DOCTOR TO THE REST OF THE GARDEN. I THIBJ THE K BICARB HAS BEEN DOING GOOD KEEPING THE PM DOWN ON THE MIDDLE GMO. I HATE THAT I HAVE TO FIGHT DISEASES. IM NOT DOING CLONES AGAIN. AND IM GOING TO BE MUCH CLEANER. THE SHERB PIE LOOKS A LITTLE OVERWATERED AND HAS SOME YELLOW LEAVES BUT MANY HAVE TURNED PURPLE AND OTHER FALL COLORS. THE PLANTS ARE AGGRESSIVELY FLOWERING AND TEMPS ARE CHANGING. STILL IN EARLY FLOWER. IVE GOT A GOOD FEELING. IVE DEALT WITH MUCH WORSE AND CAME OUT GOOD.
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Welcome growfessors to my outdoor 2021 grow! It's been 4 years since my last outdoor grow. We've got LSD and Green Crack outside, each one in half a 55 gallon container, in pro-mix HP, with Gaia Green 4-4-4 all purpose powder nutrients. Thanks for stopping by growfessors 👽🌳💚
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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@fiognarr
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did a bunch of defoliating right before the pictures, it was wicked dense. two waterings ago it got .5ml fox farm grow big in 5gallons of water. it really hasnt needed nutes yet.
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@nonick123
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Día 51 (22/07) Las plantas han reaccionado bien al 2º topping! 💥 Algunas de ellas ya empiezan a formar los nuevos brotes justo debajo del nudo cortado! Hoy NO necesitan riego, tras regar ayer con 1 Litro de Té Vegetativo de Lurpe! 💨😁 Día 52 (23/07) Las plantas empiezan a formar rapidamente nuevas ramas tras el topping! 😍 A pesar del alto calor (35 - 38 ºC), gracias al sombreado y el mulch se mantienen frescas y con la tierra humeda, ¡perfecto para los microorganismos del living soil! Riego: Solo es necesario regar a OnionOG #1 con 1 Litro de H20 pH 6,5 Día 53 (24/07) Riego: Solo es necesario regar a OnionOG #1 con 1 Litro de H20 pH 6,5. Esta planta se muestra muy sedienta comparada con el resto! Día 54 (25/07) Los nuevas ramas se lanzan al cielo! 💥 Me encanta ver como los nuevos brotes de un color verde palido (casi radiactivo) nacen entre el intenso verde oscuro de las hojas de abanico ya formadas Hace un dia nublado y con 29 ºC de maxima. No es necesario regar! Día 55 (26/07) Riego con 500 ml H2O pH 6,5 Día 56 (27/07) Retiro los ajustes de LST a la mayoria de las plantas, para que crezcan libres ahora que se acerca el stretch No es necesario regar Día 57 (28/07) Riego con 1 Litro H2O pH 6,5 con 25 ml/L de Humus de Lombriz Liquido Aplicación foliar Kelp hidrolizado de Lurpe Solutions a 0,25 ml/l 💦Nutrients by Lurpe Solutions - www.lurpenaturalsolutions.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae
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Started having some leaf issues at the end of week 6, defoliated, gave medium/heavy feeding of big bloom. Going to start flushing with distilled water for a few days and see if any improvement, only fan leaves affected at this time. Day 45: checked ph of soil, showing 7.0, just ordered some fox farms ph down should be here in 48hrs Day 49: bought a reliable pH testing pen and found my distilled water to be 9.5! Mixed up some spring water/nutes/pH down at 5.8/5.9 . Hopefully this does the trick!
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@Enki_Weed
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Die letzte Woche vor der Ernte gibt's nur noch Wasser, CalMag und einen Tropfen SuperVit. Die Buds sind so schwer, dass die Pflanze schon schräg hängt, und der Duft ist einfach mega lecker!😋 The last week before harvest will only involve water, CalMag, and a drop of SuperVit. The buds are so heavy that the plant is already leaning to one side, and the scent is just incredibly delicious!👍
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Hallo zusammen 🤙. So das war es für sie habe sie heute geerntet Sie riecht fantastisch und sieht sehr lecker aus. Wir sehen uns in 3 Wochen mit dem Erntebericht. Rabattcode für den BIOTABS-Webshop https://biotabs.nl/en/shop/ GDBT420, damit erhalten Sie 15 Prozent
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@Weedinho
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Love this strain. Récord for my personal production per plant 🙌🏻
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Lots of growth this week! 😃 So far, the indoor girls are looking fuller to me. They seem shorter with tighter nodes and more nodes than the outdoor girls. Also one of the outdoor girls seems to be a little bit of a runt. I'm hoping she catches up eventually, but I'm noticing the runty looking girl is also not symmetrical for some reason. I think this is a good comparison. I'm glad I'm growing 2 outdoors and 2 indoors so we can see a more fair comparison. If I only had one of each it might be a genetic thing causing the differences. Btw... the indoor plants are under 315w CMH on a 19/5 light cycle, 24" from the canopy. The outdoors are obviously dependent on the sunlight which is between 15-16 hours here now. I don't care that the outdoor girls have stretched more than the indoor - they don't have a ceiling to deal with, nor will they grow too close to the light (unless I'm really lucky 😉 ). I am very happy that so far I do not see any damage due to bugs on any of the plants. I'm still feeding at 75% of the Fox Farm schedule. That seems to be the right level to me. I'm almost out of Big Bloom, just ordered another gallon. What do you guys think? Please comment! Thanks!!!
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Got the new Mars Hydro 3000 this week and we’re super excited! Transplanted to 1 gal pots
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@Pungolian
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Stretching has ended finally and budding and stacking has started, yay
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@GrowGuy97
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Day 35, start of week 2 in flower, everything is going great so far✌️🏼🌱 Day 37 - All the ladies are looking great✌️🏼🌱 Day 38 - ladies got watered today, looking good & starting to put off a nice smell😍 Day 40 - Still have a lot of yellowing leaves towards the bottom of almost all the plants can’t figure out exactly what’s wrong😅😅