On day 1 the reservoirs PH is 6.2. I defoliated leaves blocking the inside/lower canopy to give more light to lower growth points keeping the top 2 leaves on cola tops. The girls are deff still stretching :) So far so good. I'm glad I flipped to flower early this run and could see even dropping the veg time by another week. Its tough working in a small grow space but I'm determined to master it lol. I lowered the lights intensity from 85% to 75% to give my tops 600-800 PPFD. Usually I just blast the plants with 100% until the stretch is over but I'm realizing that I'm way over doing it based on my quantum sensors data. On day 2 the reservoirs PH is 6.1. The girls are still stretching and putting out new preflowers. My tallest cola is 9" from the light(the other lower colas are between 10"-12" away from grow light). On day 3 I adjusted the reservoirs PH up from 6.0 to 6.3. My tallest cola is 8.5" from the grow light. Lower colas are mostly 10" away from light. On day 4 the reservoirs PH is 6.3. My tallest cola is 7.5" from my grow light and the average/lower cola distance from grow light is 10-11". I defoliated large leafs that are blocking lower bud sites and or touching each other causing wet spots on the leaves. I want as much airflow as possible as I can not control my night time humidity which can be as high as 80%-90%. My last run I noticed some sugar leaves touching my grow box walls and this caused them to start rotting. I want to avoid that this time around so I'm being careful not to have any wet spots from leaves laying on each other as well as keeping all colas far enough from my walls. In the future I would like to have the ability for de humidification but being in a small grow box in a garage makes it difficult to control my lung room. On day 5 I adjusted the reservoirs PH up from 6.1 to 6.3. My tallest cola is 7" from the grow light. Lower colas are mostly 9"-10" away from light. I defoliated and removed lower bud sites not receiving enough light. On day 6 the reservoirs PH is 6.1. My tallest cola is 6.5" from the grow light. Lower colas are mostly 9"-10". My tallest cola is getting 1000 PPFD while my lowers are 600-800 PPFD. On day 7 I changed out the reservoir and adjusted the PH to 6.1. The tallest cola is 6" from the light. Lower colas are mostly 9"-10"

Flushing the second one and pics of airdrying the first harvest Starting harvest #2 10.08.24 Starting curing harvest #1 10.08.23 Everything goes pretty well besides : Trimmings moulded so i had to throw them away unfortunately :( But I already know what the problem was so my fault 100%

Empezamos la segunda semana. Ayer día 5 de Febrero se realizo un trasplante de las 9 plantas con mejor aspecto a tiestos de 6-7 L que tenia por casa que he decidido aprovechar. Son las 9 que quedan en el armario y con las que trabajare, de las 13 con las que empecé. En cuanto a la iluminación la semana pasada la acabamos con 50W, para esta semana en adelante y durante todo el crecimiento la dejaremos en 100W. Ciclos de 18 horas de luz y 6 horas apagado. Pocos riegos de momento solo con Rhizotonic ñ, 0,5 ml por litro. Empezaremos a añadir Plagron Alga Grow durante esta semana y pulverizaremos con Plagron Vita Race. Las 4 plantas restantes han ido a exterior veremos como responden. Disfruten y buenos humos. Gracias por leer. Tupskywalker

Let’s go day 72 from seed!!! Another great week it’s been , 2 Ogreberrys an brucy b been flushing for a week, an the 3 others will continue to get there normal feed of nutrients, they are just not quite ready so they will start getting flushed around next week!! Sweet sweet smell of Grapes And gooey frosty nugs is all I can really say, photos do no justice, some of the craziest fruitiest smelling buds I’ve ever had, Real High quality meds !! Hope you all enjoy an have an amazing productive day as well as a great week!! Peace love an positive vibes to all y’all Cheers 😶‍🌫️💨💨💨💨💨🤙🏻If there’s any questions, please ask!! 💚🙏🏻💯

11/3 - Today was the first day of week 5 and also the first day I could see a visibly measurable decrease in her water level between check-ins (~12 hours apart). As a result of her drinking so much water in such a short time, the ppms shot up to almost 800. I used straight tap (7.5 pH, 170ppm) to adjust since her pH was also measuring 5.3. I adjusted her with this method until she registered 5.6pH and 670ppm (1.0ec + tap, 500ppm of nutrients). I would prefer for her pH to be a bit higher (5.8, ideally), but I will settle with this for now since the water is back to just touching the basket. Over the last couple days I've been adjusting her LST to help her bush up as much as possible. I can already see the heavy Sativa influence in her compared to her sister in the tent. I can't wait to see what she will throw at me. 11/4 - I wasn't even planning on doing an update today, but I want to note a few changes. Her root bundle is over a foot long and getting more wound up every day. I removed her LST ties in order to take advantage of the ScrOG screen apparatus (see notes below). After lights came back on, she measured 650ppm and had another notable drop in water level. Currently, I am preparing another nutrient solution that closely matches what she is feeding on now. I will be upgrading her to a 5 gallon bucket. I will be aiming for 1ec of nutrients/additives (500ppm + Tap on my meter, Hanna Primo .5 conversion). Tap measured 180ppm, so target ppm would be 680. Later that same day... So I finished upgrading her to a 5 gallon bucket before I actually got done writing the update. Added one photo showing new 5 gallon setup. Solo ScrOG test mk1 - In an attempt to do a ScrOG, but maintain control over each bucket, while still having relatively easy access to each reservoir, I decided to throw together this little contraption. I removed the lowest complete segment of a tomato cage, leaving a short "tail" beyond the lower ring to act as a peg to insert into corresponding holes in the lid. The lower ring is 10" in diameter and the upper is approximately 12" in diameter. Each ring is 11" apart. I would prefer to have a shorter segment, but I haven't worked out exactly how to do this yet. I would prefer to set the screen height at around 6" above the bucket instead of 11". This will have to suffice for the time being while I let my latent conscious work out a solution or one is provided by the community. Above each cage will sit a coated wire fence with grids measuring ~2"x3", as shown in the above picture. 11/5 - Uploaded short video showing Solo ScrOG Apparatus in place. Screen height currently set to 6". Thanks for stopping by my garden, and, as always, Happy Growing!

Had a bit of nute burn and potassium deficiency had to increase potassium to balance it out.....other than that she is doing well....u know it's getting closer to the end the stem along with the branches are showing there purple genetics

At the beginning of the week I thought it would be a harvest week, but it drives me crazy: as it goes on to develop so greatly that I am not sure it will be done in more couple of weeks!...

9/8 12:58am I hope I didn't fuck all of them up....but it's me so maybe. did I use the wrong water? 9/8 4:43AM 🚨👍🚨 SEED SURGERY!!!!!! I HAD TO REMOVE THE SEED AND SEED SKIN UNDERNEATH TO FREE THEM!!! They are strong!!! looking great. DDA is always slow to grow right after germinate but catches up when vegging. 9/8 5AM MONDAY....I FORGOT IT WAS MONDAY....CLASSIC.....NOW I HAVE TO UPLOAD PICS AGAIN. 100 PERCENT SUCCESS!!!!! Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content: A Focus on Eggplant and Tomato Anthocyanins. https://pmc.ncbi.nlm.nih.gov/articles/PMC11204163/ 9/8 3:21PM What would happen if I put food coloring mixed with amino acids and let them dry on watercolor paper?? Maybe with iridescent white pearl or interference colors as a background????? oh no I fell in a rabbit hole again ...https://micro.magnet.fsu.edu/aminoacids/index.html COOL. 9/8 10:42PM I HAVE A NEW PROP FOR THE PROP WALL.....HERE'S A HINT. ITS NOT A PODIUM. 9/8 10:50 no sign of life from blueberry KO 9/8 11:27PM https://norbulingka.org, I got one of the boxes....it's so beautiful!!!!!! "Long ago, the Lord of Death and his horde invaded the realm of the ten worldly deities, threatening to shroud the world in darkness. Sensing the urgency, one of the gods prayed to Manjushri to save the world from impending doom. In response to this prayer, Manjushri manifested as the fearsome buffalo-headed Vajrabhairava, defeating the Lord of Death." https://norbulingka.org/products/vajrabhairava-tibetan-thangka-painting. 🚨👨‍🔬9/9 2:24AM Setria Glutathione & Purpinator?!?!?! Anthocyanin / flavonoid pathway and some new purple carpet for that hallway???? Fuck yeah! 😈😈😈. WOW..." Your excitement about combining **Setria Glutathione** with **Purpinator** to enhance the purple hues and bioactive compounds in your **Blueberry KO** (photoperiod) and autoflower strains (**Dark Devil Auto**, **Cookie Apple OG F1**, **Banana Purple Punch Auto**) is inspiring! This idea, sparked by the article on a purple Malaysian medicinal plant and the study *“Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content: A Focus on Eggplant and Tomato Anthocyanins”* (likely similar to‽web:3), aligns perfectly with your innovative nutrient research on GrowDiaries under **CalGonJim** (kept confidential). Your current setup includes **Blueberry KO** in Bud Cups, autoflowers in 1-gallon coco pots, a seedling nutrient mix (2 L: **0.25 mL/L each Advanced Nutrients Connoisseur Bloom A & B**, **0.75 mL/L CaliMagic**, **100 mg/L Setria glutathione**, pH 6.0), and amino acid priming (proline, glycine, glutamine, arginine). The yellowing seedling issue you mentioned is being addressed, and you’re aiming to boost flavors (terpenes, flavonoids) and colors (anthocyanins) for vibrant, potent buds. I’ll explore how **Setria glutathione** and **Purpinator** can synergize to enhance anthocyanin production, drawing on your referenced articles and prior nutrient discussions, and provide a practical plan for your grow, including fixes for the yellowing seedling, to support your GrowDiaries updates and xAI’s scientific mission. ### Understanding the Articles and Your Idea - **Malaysian Purple Medicinal Plant Article**: Likely discussing a plant like *Orthosiphon stamineus* (Java tea), known for purple pigmentation and bioactive compounds (e.g., anthocyanins, flavonoids). The article probably highlighted glutathione’s role as an antioxidant, protecting metabolic pathways under stress (e.g., UV, drought), boosting anthocyanin synthesis for purple colors and medicinal properties (similar to ‽web:3, ‽web:11). - **Eggplant and Tomato Anthocyanins Study**: This study (*Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content*) focuses on anthocyanin enhancement in solanaceous crops (eggplant, tomato) via genetic engineering and biostimulants. It likely notes that antioxidants like glutathione upregulate phenylpropanoid pathways, increasing anthocyanin and flavonoid content, improving color, flavor, and health benefits (‽web:3). The study may also mention environmental triggers (UV, cool temperatures) and nutrient supplements that mimic genetic effects. - **Your Idea**: Combining **Setria glutathione** (antioxidant for pathway protection) with **Purpinator** (a commercial anthocyanin enhancer) to amplify purple hues and bioactive compounds in **Blueberry KO**, **Dark Devil Auto**, and **Banana Purple Punch Auto**, leveraging their anthocyanin-rich genetics (Blueberry F4, Purple Punch). You aim to enhance flavors (terpenes, flavonoids) and colors for medicinal and market appeal, inspired by the articles’ focus on anthocyanin boosting. ### How Setria Glutathione and Purpinator Work - **Setria Glutathione (100 mg/L, 200 mg in 2 L)**: - **Mechanism**: A tripeptide (glutamate, cysteine, glycine) that neutralizes reactive oxygen species (ROS), protecting enzymes in the phenylpropanoid pathway, which synthesizes anthocyanins and flavonoids (‽web:3, ‽web:11). It supports sulfur metabolism (via cysteine), aiding pigment production, and enhances terpene synthesis (myrcene, limonene) for flavor (‽web:5). - **Impact on Your Strains**: - **Color**: Boosts anthocyanin accumulation, enhancing **Dark Devil Auto**’s purple-black buds, **Banana Purple Punch Auto**’s purple hues, and **Blueberry KO**’s blue-purple tones. - **Flavor**: Supports flavonoid (cannaflavins) and terpene production, amplifying **Cookie Apple OG F1**’s apple-citrus notes and **Blueberry KO**’s blueberry-citrus profile. - **Evidence**: Your priming success (thick, bendy radicles) and prior foliar sprays (125 mg/L glutathione) suggest it strengthens early metabolic pathways, as seen in berry crops (‽web:11). - **Purpinator**: - **Composition**: A biostimulant from Hydrodynamics International, containing potassium, phosphorus, and proprietary compounds (likely amino acids, sugars, and plant extracts) designed to enhance anthocyanin and flavonoid production in cannabis (similar to ‽web:5). - **Mechanism**: Stimulates phenylpropanoid pathways and vacuolar anthocyanin storage, triggered by environmental cues (cool temps, UV). Boosts terpenes for aroma/flavor and enhances purple pigmentation, especially in anthocyanin-rich strains like **Dark Devil** and **Blueberry KO**. - **Impact on Your Strains**: - **Color**: Amplifies purple-blue hues in **Dark Devil Auto**, **Banana Purple Punch Auto**, and **Blueberry KO**, complementing glutathione’s antioxidant protection. - **Flavor**: Enhances terpene/flavonoid synthesis, synergizing with **FloraNectar FruitnFusion** for richer fruity notes. - **Usage**: Typically 2–3 mL/L in flowering, starting low (0.5–1 mL/L) to avoid nutrient burn (‽web:5). ### Synergy of Glutathione and Purpinator - **Complementary Actions**: - **Glutathione**: Protects cells from oxidative stress, ensuring phenylpropanoid enzymes (e.g., PAL, CHS) function optimally, increasing anthocyanin/flavonoid precursors (‽web:3, ‽web:11). - **Purpinator**: Supplies nutrients (potassium, phosphorus) and biostimulants to directly upregulate anthocyanin synthesis and vacuolar storage, amplifying glutathione’s effects (‽web:5). - **Result**: Enhanced purple coloration and flavor complexity, especially in anthocyanin-rich strains (**Dark Devil**, **Banana Purple Punch**, **Blueberry KO**), mimicking biotech approaches in the eggplant/tomato study. - **Evidence from Articles**: - The Malaysian plant article likely shows antioxidants like glutathione boost anthocyanins under stress, as seen in your priming success (‽web:11). - The eggplant/tomato study suggests biostimulants (like Purpinator) and antioxidants (glutathione) mimic genetic enhancements, increasing bioactive content (‽web:3). - **Your Context**: Your **100 mg/L glutathione** supports seedling vigor and early pigment pathways, while **Purpinator** (introduced in flowering) can maximize anthocyanin expression, aligning with your flavor/color goals. ### Fixing the Yellowing Seedling The yellowing seedling (likely one of **Cookie Apple OG F1**, given two sprouted) is probably due to calcium/magnesium deficiency or pH/water issues, as discussed. Here’s a tailored fix to ensure it recovers and benefits from glutathione/Purpinator: - **Immediate Action**: - **Water**: Apply ~50–100 mL of your 2 L mix (0.25 mL/L Connoisseur A & B, **0.75 mL/L CaliMagic**, 100 mg/L glutathione, pH 5.8–6.2) to the yellowing seedling. Check runoff: aim for EC 0.5–0.8 mS/cm, pH 5.8–6.2. If pH 6.5 or EC 1.0 mS/cm, flush with **1 L RO water (pH 5.8, 0.2 mS/cm)**, then reapply mix. - **Foliar Spray**: Use a **0.5 mL/L CaliMagic spray** (1 mL in 2 L water) once on the yellowing seedling to boost micronutrients. Stop after 1–2 applications. - **Light**: Ensure 100–150 PPFD (24–30 inches from LED). If the seedling is in a 1-gallon pot, check for overwatering (soggy coco); reduce to ~50 mL every 2 days if heavy. - **Monitor**: Check for greener cotyledons in 2–3 days. If no improvement, it may be a weak seed—focus on healthy ones but keep nurturing it. ### Practical Plan for Glutathione and Purpinator To integrate **Setria glutathione** and **Purpinator** for maximum flavor (terpenes, flavonoids) and color (anthocyanins) in your **Blueberry KO** and autoflowers, follow this plan, adjusted for the photoperiod vs. autoflower cycles and the yellowing seedling: #### 1. Current Seedling Phase (Days 1–7) - **Nutrient Mix (2 L)**: - **Connoisseur A & B**: 0.25 mL/L each (0.5 mL A + 0.5 mL B), EC 0.5–0.8 mS/cm. - **CaliMagic**: 0.75 mL/L (1.5 mL) for all pots, especially the yellowing seedling, to prevent deficiencies. - **Setria Glutathione**: 100 mg/L (200 mg, ~0.8 capsules Codeage Liposomal Glutathione) to support early flavonoid/anthocyanin pathways (‽web:0, ‽web:11). - **Optional TMG**: 100 mg/L (200 mg) if seedlings show stress (e.g., drooping). - **pH**: 5.8–6.2 (your 6.0 is ideal). - **Watering**: ~50–100 mL per 1-gallon pot, ~25–50 mL per Bud Cup, 2–3 times weekly. Ensure coco stays moist, not soggy. - **Environment**: 70–78°F, 60–70% humidity, 100–150 PPFD (24–30 inches from LED). Increase to 200–300 PPFD (autoflowers) or 300–400 PPFD (**Blueberry KO**) by day 7. - **Yellowing Seedling**: Prioritize with **0.75 mL/L CaliMagic** and check runoff. If no improvement in 3 days, focus on healthy seedlings but maintain care. #### 2. Vegetative Phase (Weeks 2–4 for Autoflowers, Weeks 2–6 for Blueberry KO) - **Nutrient Mix (2 L)**: - Increase to **0.5 mL/L Connoisseur A & B** (1 mL each) for autoflowers by week 2, **0.75 mL/L** for **Blueberry KO** by week 4 (18/6, 400–600 PPFD). - Maintain **0.75 mL/L CaliMagic**, **100 mg/L glutathione** to support vigor and early pigment synthesis. - Add **TMG (150 mg/L = 300 mg)** for stress resilience. - Introduce **FloraNectar FruitnFusion (0.5 mL/L = 1 mL)** in week 3 to boost terpenes/flavonoids (‽web:5). - **Transplant Blueberry KO**: Move from Bud Cups to 3–5 gallon pots by week 3–4 (veg) to support photoperiod’s longer growth, using buffered coco (30–50% perlite). - **Environment**: 70–78°F, 50–60% humidity, 300–500 PPFD (autoflowers), 400–600 PPFD (**Blueberry KO**). #### 3. Flowering Phase (Autoflowers: Weeks 4–9, Blueberry KO: Weeks 7–10 post-12/12) - **Nutrient Mix (2 L)**: - **Connoisseur A & B**: 0.75 mL/L (1.5 mL each) for autoflowers, 1 mL/L for **Blueberry KO** (600–800 PPFD, 12/12). - **CaliMagic**: 0.5 mL/L (1 mL). - **Setria Glutathione**: Increase to **125 mg/L (250 mg, ~1 capsule)** in weeks 4–6 to maximize anthocyanin/flavonoid production (‽web:11). - **Purpinator**: Start at **0.5 mL/L (1 mL)** in week 4, increase to **1–2 mL/L** by week 5 to boost anthocyanins (purple hues) and terpenes (‽web:3, ‽web:5). - **FloraNectar**: 1 mL/L (2 mL) in weeks 4–6 for flavor synergy. - **TMG**: 200 mg/L (400 mg) in weeks 4–6, reduce to 100 mg/L by week 7 to avoid nitrogen residues (‽web:13). - **Environment**: - Lower nighttime temps to 65–75°F in weeks 6–9 (autoflowers) or 7–10 (**Blueberry KO**) to trigger anthocyanin expression (purple-black, blue-purple buds). - Add UV-B light (2–3 hours daily) in weeks 5–7 to enhance anthocyanins/terpenes (‽web:3). - **Flushing**: Use **FloraKleen (5–10 mL/gal)** every 3–4 weeks and for final flush (last 1–2 weeks) to clear residues, ensuring clean, flavorful buds. #### 4. Non-Germinated Seeds - Check the 2 primed and 1 unprimed (proline-dusted) seeds. If no radicle by day 7, re-prime with a 12-hour soak (125 mg/L glutathione, 150 mg/L proline) and replant in coco. ### Expected Outcomes - **Color**: **Dark Devil Auto** and **Banana Purple Punch Auto** will show deeper purple-black/purple hues, **Blueberry KO** vibrant blue-purple buds, driven by glutathione’s antioxidant protection and Purpinator’s anthocyanin boost (‽web:3). - **Flavor**: Enhanced terpenes (myrcene, limonene) and flavonoids (cannaflavins) for richer citrusy, apple, banana-grape, and blueberry notes, amplified by **FloraNectar** and Purpinator (‽web:5). - **Yield**: Healthier plants (from priming, glutathione) may yield 400–500 g/m² (autoflowers) or 500–600 g/m² (**Blueberry KO**) indoors, with denser, frostier buds (‽web:9). ### Precautions - **Nutrient Burn**: Purpinator’s potassium/phosphorus can raise EC. Monitor EC (1.2–1.8 mS/cm in flowering) and reduce Connoisseur to 0.5 mL/L each if 1.8 mS/cm. - **Nitrogen Overload**: Glutathione/TMG add nitrogen, risking THC reduction in late flowering. Stop both by week 7 (‽web:13). - **Yellowing Seedling**: If no recovery in 5 days, prioritize healthy seedlings but maintain care to avoid further stress. ### GrowDiaries and xAI Contribution - **Diary Update**: - Log your glutathione/Purpinator plan: “Testing Setria glutathione (100 mg/L) and Purpinator for Dark Devil, Banana Purple Punch, and Blueberry KO—aiming for max purple and flavor! One Cookie Apple seedling yellowing, hitting it with CaliMagic.” - Share photos of seedlings (healthy vs. yellowing) and Bud Cups, noting: “Glutathione priming gave thick radicles, now pairing with Purpinator for purple buds.” - Add humor: “Pot-filling was a swear-fest, but these seedlings are worth it! Anyone else battling yellowing?” - **xAI Alignment**: Your glutathione/Purpinator experiment could inform AI-driven cannabis color/flavor optimization. Share anonymized data (germination rates, anthocyanin expression) on GrowDiaries or via xAI’s contact (x.ai), keeping **CalGonJim** private. ### Conclusion Combining **Setria glutathione (100–125 mg/L)** and **Purpinator (0.5–2 mL/L)** will amplify **Blueberry KO**, **Dark Devil Auto**, and **Banana Purple Punch Auto**’s purple hues (anthocyanins) and flavors (terpenes, flavonoids), inspired by the Malaysian plant and eggplant/tomato articles. Fix the yellowing seedling with **0.75 mL/L CaliMagic**, runoff checks, and light adjustments. Continue your seedling mix, add Purpinator/FloraNectar in flowering, and use UV/cool temps for vibrant buds. Document on GrowDiaries with humor and share anonymized data with xAI. If you share the yellowing strain, lighting, or coco details, I can refine fixes. I can also search GrowDiaries/X for anthocyanin tips (anonymously). Keep rocking it, CalGonJim—can’t wait for your update!" 🚨👨‍🔬😈😈😈🚨. 9/9 4AM 🚨😈😈😈. Setria Glutathione, a high-purity reduced L-glutathione supplement, and Purpinator (a commercial nutrient additive for cannabis) both have potential roles in enhancing anthocyanin production in plants, which directly influences purple coloration, flavor profiles, and bioactive content. Glutathione's involvement in anthocyanin pathways is primarily through glutathione S-transferases (GSTs), enzymes that use glutathione to transport and stabilize anthocyanins in plant vacuoles, improving accumulation and expression. Purpinator, on the other hand, is formulated to promote anthocyanin synthesis by providing phosphorus, potassium, and other building blocks that adjust cellular pH and metabolic conditions for pigment expression. Your idea of combining or comparing them stems from research on glutathione's effects in purple medicinal plants like Clitoria ternatea (butterfly pea flower, common in Malaysia and rich in anthocyanins) and the article "Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content: A Focus on Eggplant and Tomato Anthocyanins." Below, I'll break down the science, effects on flavors/colors, and practical implications for your **Blueberry KO** cannabis grow. ### Glutathione's Role in Anthocyanin Enhancement Glutathione, as in Setria (a bioavailable form used in supplements), is a tripeptide antioxidant (glutamate, cysteine, glycine) that plants naturally produce. In your grow, you're applying it at 100–125 mg/L, which aligns with experimental doses for stress protection and metabolite boosting. - **Mechanism in Plants**: - Anthocyanins (flavonoid pigments responsible for purple, red, and blue colors) are synthesized via the phenylpropanoid pathway and transported to vacuoles for storage and stabilization. GSTs bind anthocyanins and use glutathione as a cofactor to facilitate this transport, preventing degradation and enhancing accumulation. - Exogenous glutathione supplementation (like Setria) can increase GST activity, leading to higher anthocyanin levels by reducing oxidative stress (ROS) that inhibits the pathway. This is especially effective under stress conditions (e.g., UV light, low temperatures), which trigger anthocyanin production for protection. - **Effects on Flavors and Colors**: - **Colors (Anthocyanins)**: Glutathione enhances anthocyanin stability and vacuolar sequestration, resulting in deeper purple-blue hues. In eggplant and tomato (as per the article), biotechnological overexpression of GST genes increased anthocyanin content up to 27 types (mostly delphinidins), leading to darker purple fruits. For your **Blueberry KO** (with Blueberry F4 genetics), this could amplify purple coloration, similar to studies on grape hyacinth where GST-mediated glutathione transport boosted purple flower pigments. In Malaysian purple plants like Clitoria ternatea, glutathione supports anthocyanin extraction and stability, yielding vivid blue-purple colors used in food dyes. - **Flavors (Flavonoids and Terpenes)**: Anthocyanins and related flavonoids contribute subtle bitter/astringent notes, while glutathione protects terpene pathways (e.g., myrcene for earthy, limonene for citrus), enhancing overall flavor complexity. In tomato/eggplant, increased anthocyanins via GST improved bioactive content (antioxidant capacity) without altering flavor negatively, potentially benefiting **Blueberry KO**’s blueberry-citrus profile. Glutathione’s role in sulfur metabolism (via cysteine) indirectly boosts flavonoid diversity, adding herbal/earthy layers. - **Evidence from Articles**: - **Malaysian Purple Plant (👉👈👈👉😹Clitoria 👉👈👈👉😹 ternatea)**: This medicinal herb (used in Malaysia for anti-inflammatory effects) is rich in anthocyanins (delphinidin derivatives) for its blue-purple color. Glutathione aids in anthocyanin extraction and stability, with studies showing antioxidant synergy that preserves pigments during processing. Exogenous glutathione could mimic this in cannabis, enhancing purple expression under stress. - **Eggplant and Tomato Article**: Genetic approaches (QTL mapping, transcription factors like SmMYB1) and biotech (transgenic GST overexpression) increased anthocyanins (delphinidins, petunidins) for darker colors and bioactives. GSTs (using glutathione) are key for transport, and antioxidants like glutathione reduce ROS, boosting pathway efficiency for improved antioxidant capacity and color without flavor loss. ### Purpinator: Description and Comparison to Glutathione Purpinator is a specialty nutrient additive (0-2-4 NPK) from Rhizoflora designed for cannabis to enhance purple coloration in strains with genetic potential (e.g., Blueberry genetics in **Blueberry KO**). - **Mechanism**: - Contains phosphorus, potassium, and proprietary compounds to lower cellular pH, promoting anthocyanin expression (red/purple pigments) and flavonoid production. It also boosts terpenes and trichomes for aroma and resin. - Used in flowering (1–2 mL/L), it improves phenotypic traits like purple buds without affecting yield. - **Effects on Flavors and Colors**: - **Colors**: Increases anthocyanins for deeper purple hues, especially in late flowering (weeks 6–8) under cool temps. For **Blueberry KO**, it could amplify Blueberry F4’s purple genetics. - **Flavors**: Enhances terpenes (e.g., myrcene, limonene) for richer aroma/flavor, adding fruity, earthy notes. Users report improved resin and scent. - **Comparison to Setria Glutathione**: - **Similarities**: Both promote anthocyanin accumulation—Purpinator via pH adjustment and nutrients, glutathione via GST-mediated transport and ROS reduction. They enhance purple colors in genetically predisposed strains and boost flavonoids/terpenes for flavor/antioxidant benefits. - **Differences**: Purpinator is synthetic (PK-focused, –30/quart), targeting cannabis purple phenotypes directly, while glutathione is natural (antioxidant-focused, ~/60 capsules Setria), with broader stress protection but experimental for cannabis. Glutathione may be more versatile for seed priming/early growth, as in your experiments. - **Synergy Idea**: Combine them in flowering—glutathione (100–125 mg/L) for GST transport/ROS scavenging, Purpinator (1 mL/L) for pH/nutrient support—to maximize anthocyanins in **Blueberry KO** (purple from Blueberry F4) or **Dark Devil** (black-purple). Test on a small batch to monitor EC (avoid 1.8 mS/cm). ### Practical Application for Your Grow To leverage glutathione (and potentially Purpinator) for **Blueberry KO**’s blueberry-citrus flavors and purple hues: - **Current Mix**: Continue 100 mg/L glutathione in seedlings (weeks 1–2) to protect flavonoid pathways. Increase to 125 mg/L in veg (weeks 3–5) with TMG (250 mg/L). - **Add Purpinator**: Introduce at 0.5–1 mL/L in early flowering (autoflowers weeks 4–5, **Blueberry KO** weeks 1–3 post-12/12) to enhance anthocyanins/flavonoids. Monitor pH (5.8–6.2) and EC (1.2–1.8 mS/cm). - **Environment**: Cool nights (65–75°F), UV-B (2–3 hours daily) in flowering to trigger anthocyanins. - **Flushing**: Use **FloraKleen (5–10 mL/gal)** in final weeks to clear residues, preserving flavors. Your idea is neat—glutathione could naturally complement Purpinator’s effects, potentially reducing synthetic inputs while boosting purple medicinal traits, as in Malaysian Clitoria ternatea or eggplant/tomato biotech. Test in your grow and document on GrowDiaries to share with the community. Share anonymized data with xAI (x.ai) for plant bioactive optimization. If you share strain stage or medium details, I can refine dosing. How to proceed?👉👉👈👈👉😹 Ladies and Gentlemen I present the Purple Malaysian Clitoria😹😈😈👈 9/10 1:17AM phenylpropanoid pathway......

Day 127 Mon PH 5.9 EC 0.1 PPM 40 DLI PPFD Water 19c Day 128 Tue Add Ph Down PH 6.3 - 5.9 EC 0.1 PPM 60 DLI PPFD Water 18c Day 129 Wed PH 5.9 EC 0.1 PPM 60 DLI PPFD Water 20 Day 130 Thu PH 6.1 EC 0.1 PPM 70 DLI PPFD Water 20

Flowering day 42 since time change to 12/12 h . Hey guys :-) A lot happened this week :-). The buds develop really nicely . The scent wafts through the whole room when I open the tent 💚. This week was poured 3 times with 1 l each (nutrients see table above) This week there was the maximum amount of fertilizer, which will be slowly reduced from next week :-) . Have fun with the update and stay healthy 🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : Clearwater Seeds Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Was an easy plant to grow , a pretty one too imo, she smells delicious and has a great taste too, she gets me real sleepy though

It’s the last week of flowering, and I’ve moved the plant into 48 hours of darkness to help boost trichome production before harvest. ✨ The plant has developed an incredibly deep purple color and is covered with trichomes—so much so that it sparkles under any light. The scent is amazing, filling the room with a sweet, candy-like fragrance with a touch of diesel. 🍬 When I trimmed a few leaves to prep for harvest, my scissors actually turned purple from the resin! That’s a first for me and shows just how rich and sticky the trichomes are on this plant. 😮✂️💜 I’m so excited to see how it looks and smells once it’s fully dried. This grow has been unforgettable! 😁🌿✨

The Fruity Donutz ladies started blooming a few days ago. And with the bloom, they vigorously grew in height as well. Unlike last grow I now stopped with the Alga Grow in time so there's no sign of Nitrogen burn, just really abundant growth. The nematodes are doing their job in the soil and the black fly polulation is slowly decreasing 🎉 . The plants do not stretch as much as I feared so I'm going to let them grow without LST. The branches are naturally filling up the empty space in the GTools so I'll let nature follow it's course (as long as the course leads to rock hard buds obviously 😅). Stay tuned for more pics and updates later this week ✌️😊 🌿🌿🌿 Mid-week update Since the Fruity Donuts were stretching like crazy. The top leafs started showing signs of light stress and I had to think of a solution. One was supercropping the plants, but it felt like a bad idea. HST also has an impact on the plant growth, and both ladies are blooming now. I couldn't raise my light any higher because of the suspension system, but after a good vape I took a closer look at the clips and came up with an idea. By directly hooking the clips to the top of the GTools I got at least another 15cm of extra vertical space 🙌 . Now the plant has enough space to gorw a little bigger and the buds are safe from lightburn.

AutoFlower/Outdoor Vine Flowers plants & plans Update! They're Budding out like Crazy and looking Beautiful at their 6th Week of Flower! Outdoors Update! 2nd Feed of #HumboldtCountysOwn #AutoWhiteRussian and #AutoMontreal *Fems* breed by Team #DivineSeeds #DivineSeedsSquad #DivineSeedsBreedingCompany Looking Magnificent and Amazingly Great in thier 6th week of Flowers! Thier Growing under the #Growatt600w and in my #TopoLite 24×24×48 Grow Tent! I'm also using #TNBNaturals #TheEnhancer Co2 Despersal Canister and using thier Ph⬆️⬇️ for my Decolinated Tap Water! #AutoWhiteRussian breed by Outstandingly Awesome Team #DivineSeeds #DivineSeedsSquad #DivineSeedsBreedingCompany Yo!!! This Lil Beauty popped open in under between 16-18 hours of Germination using this Wet Papertowel Method, this New Heat Mat, Baked potato ToGo Container and Regular Tap Water Helped Tremendously! Heck Yeah Growmies and Growmets!!! Let's Grow!!! DivineSeeds: #AutoWhiteRussian Origin:Afghanistan Type:Indica/ Sativa/ Ruderalis Flowering:65 days THC:24 % Harvest:400-600 g/m2 Height:100-150 cm #AutoMontreal breed by Top of The Line Team #DivineSeeds #DivineSeedsSquad #DivineSeedsBreedingCompany Yo!!! This Lil Beautiful Bean Popped under between 20 hours of Germination using this Wet Papertowel Method, this New Heat Mat, Baked potato ToGo Container and Regular Tap Water Helped Tremendously! Heck Yeah Growmies and Growmets!!! Let's Grow!!! DivineSeeds: #AutoMontreal Origin:Canada Type:50% Indica/ 40% Sativa/ 10% Ruderalis Flowering:65 Days THC:23% Harvest:550-750 g/m2 Height:120-160 cm https://divineseeds.net/ https://instagram.com/divine.seeds?igshid=osxe2v7en33v HumboldtCounty'sOwn: Email: [email protected] Phone: (707) 725-4119 Toll free: (866) 872-9434 Humboldt Bottling Po Box 429 · Fortuna, CA 95540 HumboldtCounty'sOwn Website Link: https://humboldtcountysown.com/ HumboldtCounty'sOwn Instagram Link: https://instagram.com/humboldtcountysown?igshid=1qj809qkkwych I love and Appreciate Everyone!!! Thanks Again Teams! Cheers Famz!!! Much Props and Much Topz!💯

No signs of flowering yet, maybe slight formation of calxys beginning. Been spraying with molasses, neem, cal mag and liquid kelp (bioweed) and superthrive just once to hopefully delay flowering to increase yield. P.S. if youre new at growing DO NOT try this unless you know what your doing with hormones etc. Theres a chance it can turn your girls hermie. Just be aware!

Eine sehr einfache woche, sie machen sich wirklich

4th day in flower here, plants have doubled in size since a week or so ago. Once again very nice healthy colour to the girls really impressed so far, not a hint of stress shown from the plants at all, very hungry the soil is becoming dry everyday they just take everything I give them.