FASTBERRY DAY 49 FROM SPROUT Starting to show a small shade of some nice purples. Removed the plastic wrap covering and still seems to be no more gnats.

6 week...Work goes well The plants are in full bloom and begin to fatten the flowers, discreetly satisfied with how things are going ... but I am insatiable I want more and more

Such a pleasure to grow her, she came from an auto m8 and she ended up being Photoperiod, apart from that I'm glad I've discovered her, because her buds have one of the most special and sweetest fragrance I've ever smelled in a cannabis flower, super sweet and tropical guys, such a cool strain, I definitely want to fill a lot of jars of this for my personal stash, everytime I smell her flowers makes me remember the Caribbean Islands.

👉 This year, RQS launched 5 new regular strains. The seeds are packed with10 pieces. 38 seeds out of 50 turned out beautiful (see my previous diaries). 👉 Before I put the plants into bloom, I took 2 cuttings from each plant. As soon as I knew which were the female plants, I deleted the male plants and the corresponding clones. 👉 Now I am going to raise the cuttings and flower the female plants in 5 new diaries. This diary is about the Exotic Witches # 1, # 2, # 4.

Week 9 : Day 59 : Hey all So week 9 started and some of the lady’s are bulking up , like Westcoast O.G and some of the Strawberry Pie . But the Westcoast O.G in the picture gonna be first one to harvest and also gonna give me some fat dense buds , i can see that clearly 🤤. About the Gelatos I am a bit dissapointed cause all of them 3 are like normal plants with no strong structure or a big yield sign . I hope the taste gonna prove me wrong cause I like this strains , one of my favorites . I also can’t wait to try this Westcoast O.G and Strawberry Pie again , I growed them both on one of my last runs and they turned out pretty good , especially Strawberry Pie 🍓. I hoping for a 25-30 g per plant , but I guess this gonna be a hard one this time. Like my Gorilla Glue the only one Sativa in the tent but also the slowest in growing flower and she’s eaten much lesser than the her indica sisters . But wow she got s crazy fruity smell with full of crystals on her . Cream Cookies she growed so fast in the last week , that she has come close to the other ones . I think this strain is a very fast bloomer , my last one was ready on day 69 from seed to harvest with a good amount of amber . Also a very flavoury strain 🍪 Yesterday I started to feed them Batguano , this should bring some green healthy leafs again and fat smelly buds . My first Chem on the picture is really hungry right now , I started a bit late for her but this should be ok . Updates coming

Casually working up to week two nutrients which are shown. Babies look like they handled the flip very very well considering their long strenuous lives and the extra stress they got with the shwazzing! You can see they have put on easily 1.5-2inches in some areas. I could use some more light probably going to try and get another before the end of flower. Adjusted canopy and moved some plants around for more even coverage but also easier watering and clean up for stagnant water! That will be the last time I adjust before I let them just do their thing and stretch! Also been foliar feeding fish mix every three days at .5 ml/l

Day 84 Week 6 flowering 👍🍀

we are finally geting closer to the end, had some minor over feeding issues but quickly sorted so pretty much no damage just few leaves have some signs of burns, nothing drastic one more week of normal feeding and then I will add pK 13/14 for a week and finally 2 weeks flushing I bought humidifier but It doesn't really do much work. Humidity is still around 50% so i hope good airflow will be anough to avoid any mould

Muy contento con estas geneticas de fastbuds apunto de cortar, lo proximo que vereis es cuando las corte

Exceso de nutrientes

Purple afghan kush 1...she has taken her purpyra color ... there is very little left for her to be able to taste this variety ... she has a smell of tender wood ... sweet smell ... she did not grow enough but if she dedicated herself to fattening her head ... purple afghan kush 2 she had about 15 worms ... look for them one by one ... she did not grow well from the beginning ... I do not know what happened to her growth and flowering ... her buds are small ... let's hope she tastes good ...

D68 (28/01/2021): First official day of week 6 in bloom and first official day (second in reality) of pre harvest flush. The smell is strong when I open the tent! It does not take long for the whole room to smell dank and even outside the room. - temp: 24-25C light ON ; 20-22C light OFF - no water - RH: 49-52% light ON ; 49-50% light OFF D69 (29/01/2021): I did a first proper flush today by running approximately 2.5 gallons of water trough the medium. There is still a lot of nutrients in the medium. I might do the exact same flush tomorrow. The PH of the run off is high (around PH7-7.1). This is an other reason to flush again tomorrow. I also reduced the light ON schedule by two hours. So now 18h hours ON and 6 hours OFF. I will continue reducing the ON period until they are ready for a complete dark period. I also try to reduce the air temperature. - temp: 24-25C light ON ; 19-20C light OFF - water: PH6.4, 135PPM, 2.5 gallons each - run off: PH7 and 680PPM for Glue Gelato ; PH7.1 and 560PPM for Banana Kush - RH: 47% light ON ; 48-53% light OFF D70 (30/01/2021): I did a second flush since I was not satisfied with run off PPM of yesterday. Today, the run off PPM is under 300 which is what I wanted. The run off PH is still high with 7.1. Banana Kush is ripening with the pistils slowly turning brown. Glue Gelato look sooooo yummy and I can't wait to harvest it. There is a small color change in some leaves but I'm expecting more in the next days. - temp: 23-24C light ON ; 19C light OFF - water: PH6.4, 125PPM, 2.5 gallons each - run off: PH7.1 and 260PPM for Glue Gelato ; PH7.1 and 250PPM for Banana Kush - RH: 46% light ON ; 46-48% light OFF D71 (31/01/2021): Glue Gelato is changing color. I'm checking trichomes everyday on both girls and no amber yet. - temp: 23-24C light ON ; 19C light OFF - no water - RH: 45% light ON ; 49-50% light OFF D72 (01/02/2021): Well well well...the clock is ticking for those girls. Glue Gelato is now having a couple of amber trichomes. Banana Kush don't have any. Overall Banana Kush has less trichomes and fluffier buds. This girls is not ready yet but I will need to chop her down at the same time has the Glue Gelato because my drying space is the tent I'm growing in. I will try to push the Banana Kush to ripen faster by lifting up the lamp at 16 inches from top bud and by reducing of 2 hours the day period for a 16 hours light ON and 8 hours light OFF. But at least all Banana Kush trichomes looks milky. - temp: 23-24C light ON ; 18-19C light OFF - water: PH6.2, 123PPM, less then a gallon each - run off: PH7.4 and 200PPM for Glue Gelato ; PH7.4 and 287PPM for Banana Kush - RH: 45% light ON ; 40-45% light OFF D73 (02/02/2021): I gave water probably for the last time today. Trichomes on Glue Gelato are starting to amber on top buds. Not a lot of amber but you can see that all the trichomes are starting to degrade by curling down slowly. Trichomes on Banana Kush are not amber at all but they all look milky. Glue Gelato is ready to harvest but Banana Kush need more time. It's tough to make a decision to chop or not since they are not at the same stage of growth. I want to place them in a complete 48 hours of darkness before harvest. 1h less light today for a 15h light ON and 9h light OFF. The lamps are now at 20 inches. - temp: 23-24C light ON ; 18-19C light OFF - water: less then a gallon each - RH: 45% light ON ; 40-45% light OFF D74 (03/02/2021): Glue Gelato is ready for 48h of darkness. I will take her out of the tent and place her in an other room without light. I will try to maintain a proper environment in this room and if it smells to strong or if the condition are not good I will replace her in the tent and both will start the 48h darkness. The idea is to give Banana Kush 2 more days to ripe and swoll more. After 48h, I will chop Glue Gelato and place her in the tent to start drying. Banana Kush will then start her 48h darkness. I don't know if this strategy is good but I will monitor everything closely and react if something is wrong. - temp: 22-23C light ON ; 19C light OFF - no water - RH: 47% light ON ; 43-45% light OFF

Fat and sticky....

She’s doing fine just waiting for the peak of the plant to open before I pinch her she is still going steady. She has had her wilt and stands around 20cm

The High intensity was to strong (clearly my fault) in the beginning which stunted her overall stretch, she however still delivered super dense, terpene and trichome loaded buds.

12/16 - Howdy there, growers. Here we are at the start of week 11 for this lady. Day 77 from seed. She still have a little ways to go, but she is coming along quite nicely. Buds are stacked and rock hard and getting frostier by the day. I'm trying not to rush this one. I removed the Pro-TeKt from her regimen as she isn't growing anymore. I switched her feed over to ~1.0ec (500ppm) to taper her down during her last two weeks. I am also going to taper the lighting schedule down as we approach chop on her once her sister is no longer in the tent. Thanks for checking out the garden, and, as always, Happy Growing!

4/9/25 Welcome to another week. Week 5 since the flip of 12/12 just ended. So today is day 36 since 12/12. The begining of week 6. About halfway done with flowering. Time to start packing on weight. also its important to note that it took a week or so after 12/12 to have pistils show up. So its closer to 28 -30 days of Flowering. I have a foxtailing pheno thats mostly green with thin buds. I have a super chonker green pheno with ridiculously chunked up golfball sized nugs everywhere.(R3) I have a sativa style but thicker buds with nice long connected colas (R2) another smaller pheno with traits of some of the others and also green. and then a singlular pheno that looks gelato dominant and its purple pretty much everywhere to the point that i didnt notice its coloring until it was next to all of its sisters that are straight green. Very beautiful plants and a nice display of phenotypical expressions Top dressed 2tsp bloom 2tsp microbe charge 1tsp bloom booster A Special thank you to Zamnesia and

Culture facile sans aucun soucis belle plante tres esthétique une merveille pour les amateurs de gout fruité

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.