Hello guys, Hope you are doing well. you will find into the video a review of plant before the flowering stage That's it ! it was the last week of veg stage, i'm switching now into flowering ! The box is full at 40% and this is what we want before the flowering stretch. she is very healthy and easy to grow. Hope you enjoy Take care guys and see you next week !

All in all a good run but because of my fault with the wrong lightcolour. Wont Happen again. On to the next one. (After I smoked All these)

Week 8 This week i ´ve been defoliating the cookies and stopped to give them nuts after some signs of stress on leaves, they are curling up and get burn out on their tips, this is most visible on pot 1 who´s appearing to be a sativa pheno! On the other hand Pot 2 who appears to be more indica pheno are showing more resistance to nuts! I Just been feeding them with ph water and have increase the distance for the ligths, I dont relate the burns on my leaves from that but with the temperatures on outside changing so drastic and the entire grow been heated and sealed (in order to save some money on energy) i only opened the windows to refresh the air two or tree times this week for a couple of hours generaly at 12 oclock to dont have my temps changing from 22 degres to 8 degres 😱, not the best conditions and not my easiest grow but what can i say? You can look them an judge... to me they are fucking Hero´s... They became thirsty, suffered from insufficient air flow, suffered slight burns for 24 hours of continuous light from the seed until now and still suffered from excessive fertilization but the ladies didn't fall off their high heels 🙌

Day 15 transplanted to a 10 gal container and 3×3 tent. Day 30 transitioning to flower. She's measuring 24 inches across definitely bushy and stout. Plan on scrogging through the upcoming weeks.

An Tag 43 und Tag 48 wurden beiden Pflanzen nochmal großzügig entlaubt. Viele Leute sagen "Mach das bloß nicht" und viele Andere sagen "würde ich auf jeden Fall machen". Grade bei den beiden +Speed Auto´s, die sehr klein und buschig wachsen, hielt ich dies für förderlich. So konnte ich sowohl die Lichtmenge für die wichtigen Buds erhöhen als auch gegen Schimmel vorbeugen. Viele LST Schnüre konnte ich bereits entfernen und alles wächst ungefähr in gewünschten Bahnen. Bei der höher gewachsenen Pflanze mache ich mir etwa sorgen wegen den hellen Spitzen der Blätter. Ansonsten entwickeln sich alle 4 Pflanzen ohne weitere Probleme.

Looking good hopefully all will be showing signs of flowering at the end of this week. I'll update a few more times this week.

De koningin is vandaag 21 september geoogst en in zijn geheel opgehangen in de tent. Als de toppen droog zijn zal ik de uitkomst delen. Beste Weedseedsexpress, Ik wil jullie graag hartelijk bedanken voor de kans om de Permanent Marker Autoflower te mogen kweken. Vanaf het begin groeide de plant krachtig en gezond, en de toppen die ze produceerd zijn werkelijk indrukwekkend. Ze werden zelfs zo zwaar dat ik extra ondersteuning moest bieden om de takken overeind te houden – een luxeprobleem waar ik als kweker heel blij van word. Ik ben meer dan tevreden met het eindresultaat en kan niet wachten om deze strain in de toekomst opnieuw te kweken. Nogmaals dank voor jullie geweldige service en kwaliteitszaden. Ik kijk ernaar uit om meer van jullie aanbod te ontdekken! ❤️

The Blue Zushi stretch has escalated 😅. I really can't raise the lamps any higher. The Blueberry Cheese has barely grown in height and is now about 60 cm away from the lamp. It is also about 7 days behind in development. The Blue Zushi is developing a really nice resin cover during this time I will remove a few fan leaves in the next few days so that the lower flowers get more light. there is already a lot going on in the tent. I have also installed two deep red Adlights from Mars Hydro that I had lying around. I added 40 liters to the autopot tank yesterday, but completely without nutrients. My tap water already contains a lot of calcium by itself

One more week of flush, the leaves are starting become yellow. The buds are getting fatter and frosty. Check out the video that i v made. One more thing fellas, i'v put two graphics showing an experience that i'v made measuring the ph and ec of runoff for some weeks: 1 - About PH: my soil always buffer about 1 pt of ph. Except in the day 08/08 that i made a full flush. 2 - About the EC: You can see in the start of the graphic some peaks. This is beacuse i was alternating between solution and tap water. After that i started aways put the solution in every watering and the graphic become more estable following the concentration of ec until the flush in 08/08. I dont know exactly why this buffer of ph and ec occurs. I think that it was because i'm recycling the soil over the grows. :] Next week, HARVESTTTT!

This strain was a lot of fun to grow, Training was easy, it responded well and rolled with the punches, She was CalMag hungry as that as my only noted deficiency. She was chopped at day 69, would have been 70 but I had mothers day plans with the Ol' lady. The wet weight is everything but the bigger fan leaves (larf galore) I had so much trim. I love the smell I loved growing it, but I do not like how airy the buds turned out, I use quality LEDs and have a white widow in another journal that grew along side it under the same conditions, even the same trainings to the point where there structured extremely similarly, and the White Widow is ROCK SOLID. BUT with that being said, the trichome production was very nice, the sugar leaves will leave me with a lot of hash (half the buds were considered larf but were so sticky and covered in trichomes) and the smell is very sweet, berries and a hint of lemonaid and must. Keep in mind this is a taste after just a couple days of drying a sample bud and no cure. Overall its definitely smokable ill probably give some out but I would rather keep the airy stuff for me to smoke/process because I love it all. I am currently taking the week as a T-break so I can give everyone here a proper smoke report, including myself. Dry bud weight is only 51g BUT all the trim did get me a couple of grams, probably 3 of some incredible hash, The Hash taste like blueberry pie and a glass of lemonaid, the flower is similar but with a bit of a musky punch, the high hit me straight in the head and had me laughing at myself for getting so stoned, literally all i wanted was that pie and lemonaid that's all that I had on my mind. after a bit it mellowed out into a nice relaxing sativa feel, I wasn't nearly as full of energy but I was by no means tired either.

I harvested the last plant in several steps. The results are impressive. The first fluffier one was not as productive with 301g wet and 43g dry, but I also harvested it too early for other reasons. I'm going to make myself a snack from the buds. The other one performed great. The buds are hard as stones and smell like tropical fruit gums. I'm really excited to see how it tastes! I'll update when everything is dry! 👻👽 UPDATE DRY: The run is done! The Tropicanna Poison was super easy to grow and smells simply great. The first testers almost blew me away, really strong. More than expected. I would definitely grow it again. I harvested the first one way too early on day 65 because I had problems with the humidity, so as expected only 43g dry. The beautiful but small one with the massive buds gave me 76g of super dense and delicious buds. Less than 2g of popcorn :) Of course, I only kept 49.9g of the most beautiful flowers and properly destroyed the rest. 😕

Week 1 (I think kinda confusing to a newbie) plant seems to be doing fine. Once the second set of leaves came in I moved outside for morning sun. By day 8 it spent the whole day outside. After day 9 it is officially an outside plant. I only put it in the green house at night and when it’s raining.

Northern lights looking beautiful! Really resilient! Smells like citrus and pine.

~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ ❤️💡🌱😽💨 First week of flower at last!!! This plant is sooo ready to go lol...we started her flower feeding regiment a whole week before flowering.. we also replaced x2 315watt Philip CMH fixtures for the FC-E6500 by Marshydro..what an awesome move.. this light is so f*cking incredible.. i honestly don't think a better 4x4 option exists, if it does Iv'e never seen it... this plant probably should of been flipped a few weeks back but she should have the room (hopefully)..we'd love to pull over a Lb and this is DEFINITELY the strain to do it.. we pull approx. 3-3.5oz dry on a 6 week veg with ILGM Big Bud, this should smash some records lol...Thanks for dropping by and happy harvests folks!! ⚡Mars Hydro/FC-E6500⚡ Specifications ⚙️: 650 watt, full spectrum design, removable light bars and driver 🔌 Diodes: BridgeLux chips (3978 count!) Veg Coverage: 6x6 ft 🌱 Flowering Coverage: 5x5 ft 🌼 High-Efficiency PPFD: 1200umol/m²/s ☢️ -IP65 waterproof ratings, tolerant to high humidity grow environments 💦 .. -Can be daisy-chained together and all controlled from a single light! 💡~💡~💡~💡~💡 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_

Week 11 7th week of flower. Light settings 12/12 on at 9.00am off 9.00pm Light power= 75% 184w Radiator settings LIghts on Turn on if temp drops below 22.5c Turn off if temp goes above 22.5c Lights off Turn on if temp drops below 18.5c Turn off if temp goes above 18.5c Extractor settings temperature 23c with 1c steps Humidity 50% with 0% steps Max speed 10 Min speed 4 VPD aim 1.0-1.4 DLI aim 35 Wednesday 5/4/23 Day 71 (Day 43 flower) Away Thursday 6/4/23 Day 72 (Day 44 flower) Away Autofeed 4 15 minute runs Normal pump 4 drops ph d Water Est. 24L Ec=0.22 Ph=6.3 Est. 18L left Roughly 2L each. Friday 7/4/23 Day 73 (Day 45 flower) Back home 3.30pm Movement fan has broke while away. 15L left Ec=0.225 Ph=6.7/6.7 Used15L over the week. Plants didn't like being on just water for a week. Needed PK #2 Height= 51CM Dist= 43CM DLI=27.3 Big defoliation. Lifted light and moved it. #2 Height= 51CM Dist= 49CM DLI=21.9 Saturday 8/4/23 Day 74 (Day 46 flower) Autofeed 5 runs (total 70 mins) Normal pump Different Feed times 4 drops ph d Nutes 15L Ec=1.37 Ph=6.3 8L left Roughly 2.3L each. #2 run 0.15L Ec=3.05 Ph=6.1 Radiator settings LIghts on Turn on if temp drops below 21.5c Turn off if temp goes above 21.5c Lights off Turn on if temp drops below 18.0c Turn off if temp goes above 18.0c Extractor settings temperature 23c with 1c steps Humidity 45% with 0% steps Max speed 10 Min speed 4 Been a hot day. Sunday 9/4/23 Day 75 (Day 47 flower) Nothing Monday 10/4/23 Day 76 (Day 48 flower) Nothing Tuesday 11/4/23 Day 77 (Day 49 flower) Autofeed 6 runs (2,3,5,15,15,15 total 55 mins) Normal pump Different Feed times 4 drops ph d Nutes 8L Ec=1.32 Ph=6.3 8L left Roughly 1.6L each. #2 Height= 51CM Dist= 49CM DLI=19.2 Light power= 100% 240w #2 Height= 51CM Dist= 49CM DLI=24.1 I was away for most of the week so the grow was on autopilot. I decided to have them just being watered rather then having any nutes this week. Some plants suffered a little but I got on top of it when I got home. #2 Tropicana Cookies FF has also done OK but could have done with some nutes during the week. Power used for week Light (2.21+2.22+2.21+2.21+2.22+2.21+2.25)=15.53Kw Extractor (0.55+0.57+0.55+0.54+0.58+0.58+0.58)=3.95Kw Fans (0.98+0.99+0.94+0.94+0.95+0.89+0.87)=6.56Kw Dehumidifier (++++++)=Kw Radiator (2.22+2.19+2.46+1.75+1.38+1.1+1.69)=12.79Kw Water pump. (0+0.01+0+0.01+0+0+0.01)=0.03Kw Total for the week=38.86Kw Back in a week. Thanks for stopping by

D36: (04/09/2022) Added water only to Maria (Around 300 ml). Nothing for Mario since he has root rot.

Buenas a tod@s!, bueno novena semana de flora de estás runtz x layer cake, seguramente ante última o última semana, les falta nada... La plata está hermosa, más allá de sus hojas marchitas tiene unos frutos super pretos, compactos, pesados y mucha resina... Tanbien ultima semana de nutientes. Aclarando q es mi segundo armario, y sigo teniendo algunos problemas q mejorar, siempre trabajando humildemente, planta humilde y muy hermosas, no tengo experiencia, escucho todas las opiniones, pero eso no me define, me encantan mis plantas y cada día trabajando para q sean mejor.. falta muy poco... Un saludo y buenos humos para tod@s!!! 💨💨🔥😎⚕️💪🏻 🇦🇷🤝🏻🇪🇦

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

We lost about 40 girls... we are not sure why... humidity, temperature and lamp distance are OK... only what we see, maybe tabs were too close to plant ?!??! 🤷🏻🤷🏻🤷🏻