Day 50 little defoliation not much plant is nice and healthy. Raised light again. Day 51 nothing done buds are getting bigger. Still nice and healthy. Day 52 plant is budding very nicely. All is good forgot pics for d52 so you have video. Day 53 refilled refill tank 920ppm ph'd 6.2 plant is doing great. Day 54 plant is green tips of leafs are great and she is bud building. Day 55 all is good. Day 56 plant is so beautiful I can't stop taking pictures of it.

Day 29 - Things going along nicely .... i trimmed down some of the bigger bottom leaves, i didn't have enough soil to fill the pots, and the plants are short, bushy, weird little things, that filled out the inside of the pots..... they are only 15cm tall, but the leaves are 10+ cm .... really weird little plants.... haha.... i decided to trim the fk out of one of the ones in the double pot, and practiced topping on her.... i'll cut her down maybe trimmer her of the pot, and focus on just the 2........ they seem to be still growing upwards, so i'm not in a hurry to put em into flower mode......... on the main one i noticed some neut deficiencies ... so i upped the neuts.... should be ok, i put a good heavy neut and it stopped the spots etc..... i think some bug or something got in the double pot and is livin in there, but he doesn't eat much, haha....... the main stalks are as big as my thumb and under each canopy is all the branches..... it's really strange, to go in under all the growth and see it all .... they look kinda small, but the entire under canopy of leaves is all full .... i'm moving the huge sized leaves out and pulling each branch up over them as they grow..... this is 21 THC and 19 CBD, so it's all the good stuff for medicine ... the smell is getting abit noticable and i'm hoping the exhaust fan arrives from china soon.... just got positive ventilation into a carbon fil. ... and daily air out........ i think i had the led lights too low..... so i'll raise em abit more and see if i can get em to stretch .... Day 30 -- added some video's...... daylight vids... worth watchin for a laugh ...... first grow, so idk what i'm doin, ... any help/comments appreciated Day 32 -- added some pics of the late night trim and LST,... idk looks ok.... Day 34 -- added a video...

All feeds with nutes use either a whole ratio or combination of "Veg Mix" and "Bloom Mix"concentrates DILUTED in water until a total ppm of add in is reached using a (Total Dissolved Solids)TDS Meter measured in PPM (parts per million). The "Veg Mix" concentrate will eventually be added in smaller ratios and "Bloom Mix" concentrate what will eventually replace the "Veg Mix" concentrate entirely with the ppm and ratios listed when I feed. Veg mix recipe is on week 3. Bloom Mix recipe is on week 5. Day 28 Just a quick update for this morning since I had to move the ties a little. LST, I now have them above the 3rd node branches. Stats are normal and the growth tips are turning upward now. Stats 73F/ 63% RH /IL Vent at 33% - Lights also same as before 345ppfd highest, 330 center, and 315 at lowest. No need for adjustment. She seems to be taking the training well. Day 29 Water day - checked last night and decided it was time again - Water by weight again so no runoff to measure at about 4 cups. (4 days since last feed by weight) 13lbs even par is 15lbs 4oz so I prepared 2lbs 4oz of water. Tested my meter and discovered it needed calibration (the temp is off and cant be calibrated - so I used a trusty meat thermometer. started with 4ppm and added 300ppm of epsom and vegmix ph balanced to 6.34 at 69.8F - then splashed with H2O2. Poured around roots and splashed the stem base - sprayed some on the leaves and use the whole amount. Had to adjust lights now to be brighter so I brought them down to 18.5" and ppfd is now 375highest 360 center and 345 at lowest. VPD is normal at 1.0 with 73F /63% RH /33% IL Vent - fans are pointed to directly now as the leaves are starting to get rough feeling - so I moved them a bit to blow less directly Training is doing good nice upward turn on the training (making small LST movements with the ties other than than... just letting it make new branches. Day 30 Checked PH and got 5.0 on my meter(+1.25 tested differential = 6.25) Stats normal too - 73F /64% RH /33% IL Vent - Lights at 385highest 370 center and 355 at lowest Removed cross ties since they are no longer needed to hold the fan leaves- training still looking nice as the new nodes are formed with 2nd node leaves growing already. Day 31 Identified an issue this morning - went back through my pictures, identified the start, looked at the day before, and noted my changes that I took notes on at that time. Went to google to diagnose a few possible hypothesis and am now testing with a treatment. (suggestions from more experienced growers welcome) This morning the issue was brown spots on the older fan leaves. Mostly just the left fan leaf. Seems to have started 2 days ago (day 29) when I changed the lights and fed her. When I fed her, I left water droplets on the leaves instead of drying them off after a short time (I had been drying them with a tissue after a wet pic. The fans were also adjusted after the lights were dropped closer as well. Hypothesis: I think the water was magnifying the light and caused light stress at the those spots. I dont think the wind of the fan had much to do with it other than they helped dry the leaves faster as noted by the water being completely gone by the time I took pictures later that night for measurements. Test: Raised my lights to a max of 350ppfd (from 385 highest reading this morning) Had to adjust fans again since they rest on my lights. Will observe looking for improvement over the next few days. ... another possibility was given to me by Guru @Roberts (thank you) as it possibly being a ph issue locking out calcium. So I thought I might test ph and then try diluted cal/mag in a spray if my soil is too low (also looked at pics and seen VERY close similarities in my pics and those) and so on that advice, I tested/calibrated my soil meter (it FINALLY calibrated for me!)I found my soil was avg of 3 readings at 5.153 (5.08,5.09,and 5.29) NOT GOOD - potentially locking out most all nutes. I weighed out about 2 cups of purified water with and added about half ml of CalMag, ph balanced the water to 6.5 at 69.7F (my research shows Calcium lockout starting at 6.3 or lower) and splashed with H2O2 - I poured most around the base and sprayed the leaves too. I made sure to use the whole solution by pouring the last of the solution in the spray bottle over the stem and stem base. EOD follow-up Found a possible calcium blockade in my ph and on my leaves (of which are now starting to curl so I placed my fans on oscillate to ensure they aren't doing more damage) --- fully documented now. otherwise measurements are now documented too. -- second node is starting to pull away from the last top so I will be looking to top again (3rd of possibly 4 total) and then HST and bind in a new spot Day 32 Doing a morning and evening check her now while I see this issue resolved. So far no sign of improvement since last night - might have gotten worse in comparison (will check pics side by side today) but I think because it's a immobile micronutrient so it should take time to stop then reverse. So in the mean time I am doing a slurry test as a 2nd test for my soil before I treat it. I took soil from 3 different spots and made sure I got some from about 4 inches deep in each spot (ensured calibrations were still good with buffer tests) 1st test is a PH meter tested directly in the soil. After using the 2 cups of 6.3 yesterday I got 3 readings today = 5.47, 5.32, and 5.5 to avg 5.43 - (yesterday's readings prior to treatment were 5.08, 5.09, and 5.29 to avg 5.153) - still far below the normal 6 to 7 for soil and the requirement of 6.3 and above for calcium to enter roots. suggesting again lockout. So while I am checking the plant conditions I also looked at the new nodes - 2nd node is starting to pull away from the first, but not quite enough to top yet - thinking I will be able to top tomorrow evening - should be able to correct the ph tonight, so I can see if there's results in that by then as well. I also set the fans to oscillate last night, so here's a video for that now. Measurements, ppfd, and vpd will be in tonight's check-up Day 32 So I started this evening by testing the run off with 2 meters, 5.7 and 5.67 - prepared 1 gallon of 7.5 ph water and splashed with H2O2 (checked with both meters) Before flushing, I documented the leaves again - still curled and look very dry but they still have some flexibility with them - they dont seem to getting any worse so the spray may have gotten enough to the leaves most affected. The new nodes and growth tips seem to be okay but not growing as fast as they were. That would still mean that the soil needs treatment before the roots begin to die off and I end up with stunted growth overall. SO I flushed the whole gallon and noted the runoff from the sides and bottom. I then tested the soil directly with 3 readings 5.95, 5.8, and 5.73 for avg 5.826 and tested the runoff water with 2 separate meters for 5.91 and 5.85 Not sure if I should feed dunk in 7.5 still or just use that number on the next feed in like 3 or 4 days. will discuss Other than that ppfd highest is 360, 350 at center, and 330 at lowest. VPD was 1.0 with temp at 74F /63% RH /33% IL Vent Growth tips could be ready to top again tomorrow night, but I would like the get the ph taken care of first. Day 33 Started the day by noticing the lower branches seem to stop turning and the next level branches seemed to have finally turned but nearly as fast as the first. (Noticed them having a start yesterday) Newer branches dont have any signs of issues. So the plan today is to make Bloom Juice for later. Feed Dunk with 100% Veg Juice at a ph of 7.3 test runoff and ppm. Top the 4 nodes and remove the lower leaves. Calcium issue or not, that was always the plan with main-linning the plant. However, because of the health issues, I dont think I am going to go past these 8 colas - rather instead I will simply stop here and do some HST to set them how I want them to grow and let her recover and finish her veg for about two or three weeks. Dunk had 300ppm of Epsom, Veg juice, in a little over 3 gallons of purified water. I had 7.4 ph and splashed with h2o2. I pushed the entire plant into the water and soaked for a few seconds. Drained by hanging the pot up for about 10 minutes and the stream started dripping instead. Checked the runoff with 2 meters 6.01 and 5.93 with 2580ppm. Then I topped all four nodes and removed the fan leaves from under my mainline. I used LST to finger press the new branches apart into the direction I plan to move them to get the nodes powering up. Next I will wait for a day to do HST on those new branches. Tonight I will simply let her rest. VPD still at 1.0 - 73F /63% RH /33% IL Vent - Lights are at 365 highest, 355 center, and 335 lowest. No need to measure for growth now as I dont plan to top anymore Day 34 Early entry to day for HST the morning after topping. So I started off the morning checking stats and health. PH levels are still low. 3 soil meter tests 5.41, 5.41, and 5.44 for an avg of 5.42 - kinda at a loss here but can only monitor now. VPD and lights are mostly the same - 74F /63% RH / 33% IL Vent - VPD not relevant today as the settings are the same and I am using HST to change the plant position. leaves are noted for future reference - all four lower fan leaves have signs of calcium deficiency but not progressing nearly as fast as the first two did, so I think my attempts via higher ph water and feed may have helped. (I know the microbes in the soil are supposed to help get past ph issues , but mine seem to not be heathy) HST used a cuticle rod from my wife's nail case and a paint stick to press the rod against the branches and smash the inside of the elbows I want to bend. LST I then cut 4 strips of garden wire at the same length, hooked the ends like candy canes and aligned them about 1/2 inch above the new node and into the soil. From the side they look level but have the leaves now facing sideways. They will turn in a day or two.

Just got back from holiday. Few days late on update. watering system wasn’t too shabby as it was a last minute holiday ahaha. 6 days away in Greece. Lovely. 1x Sweet Mandarine Zkittlez 1x Mimosa Bruce Banner Ready for harvest. Been put into two days of darkness!

Will give this Strain a 9/10 will come back with Dry Weight

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Note : + jegliches Zubehör wird in der GermniationsWoche aufgelistet . Zeitraffer Videos folgen immer nachträglich. + videos werden so geschnitten das nur ein geschlossenes Zelt erscheint , ergo Fehlen paar reale Minuten (ca 1 Std) . + + leider ist die Halterung defekt und die Videos etwas schief . Vielleicht mach ich mich irgendwann mal daran und korrigiere das. Sorry Day 43: + Höhe : 64cm Es wächst und wächst :)) Day 44: + Höhe : 65cm + 2 Liter Flaschenwasser , kein Dünger . + + PH 6.5 + Drain : PH 7.0 Höhe ist bei 63cm , das ist 3 cm mehr als die Vorgabe ausweisst :)) Day 45: + 66cm höhe. + 2 Liter Flaschenwasser + + Dünger für Blühte + + + Canna Terrar Flores (Achtung wirkt wie PH-) + + + Canna Boost + + + Cannazym + + + Greenhouse Feeding BioEnhancer als PH+ Jetzt säuft sie wie wilde , keine 24h und die 2Liter sind komplett wech . Mehr geht aber nicht in den Topf , ergo muss ich mir langsam mal überlegen ob ich Tropfbewässerung nutze . Und es treten duch das nur Wasser extreme "Calzium" Probleme auf . Hihi , aber so hab ich mal das ganze auf Startsequenze gesetzt . Ich arbeite also noch daran diese Mischung aus BioBizz Light und Urgesteinmehl nach Schätzung zu meistern :)) Den Fakt ist , da ist viel zu viel Urgesteinmehl drinne , was auch die Vegiphase schon gezeigt hat. Day 46: + 1.5 Liter Flaschenwasser + + Dünger für Blühte + + + Canna Terrar Flores (Achtung wirkt wie PH-) + + + Canna Boost + + + Cannazym + + + Greenhouse Feeding BioEnhancer als PH+ Day 47: Draussen zu heiss , drinnen zu feucht ... Ja genau was ich nicht brauch lol ... GoPro aus der Halterung gefallen :)) Day 48: + Höhe : 68cm + 2 Liter Flaschenwasser + + Dünger für Blühte + + + Canna Terrar Flores (Achtung wirkt wie PH-) + + + Canna Boost + + + Cannazym + + + Greenhouse Feeding BioEnhancer als PH+ Draussen zu heiss , drinnen naja ... Der Entfeuchter arbeitet weil ich sonst hier Tropenstimmung bekomme :)) Ein wenig Entlaubung . Day 49: + Höhe : 68cm + 2 Liter Flaschenwasser + + Dünger für Blühte + + + Canna Terrar Flores (Achtung wirkt wie PH-) + + + Canna Boost + + + Cannazym + + + Greenhouse Feeding BioEnhancer als PH+ + + + PH 5.8 + + Drain PH 6.5 Läuft bei der Lady , sie trinkt , liebt das Licht (auch wenn die Spitze etwas zuviel bekommt , sie betet halt etwas ) , Mangelerscheinungen sind echt gering . Hat die heissen Tage (27° im Zelt bei 60%-57% ) hat sie super überstanden . Ich werde immer besser im Gießen . 75% von oben , 25% lass ich von unten aufsaugen .

Monday 16/06/2025, start of week 5. The stretch is underway and has been for a few days, happy with how she looks health-wise currently although I've made some mistakes along the way that she has had to recover from. i made one final tuck under the net on day 29 (17/06/2025) although it's not a proper scrog by any stretch of the imagination, it's helped keep the canopy level so the side branching could catch up, and I'm letting her go now, hoping she stretches a good 20-30cm but we will see. Day 30, fed at 1.2ec, was probably 12 hours late on the watering as she did not look happy at all, bounced straight back though once fed. Day 31, not much to report, a lighter shade on the newest growth but to be expected at this stage, I've pinched a few of the lower fan leaves over the last few days. Day 32 backed the lights off slightly as the leaves were looking a little light stressed. Day 34, fed with calmag and water only at 0.4 ec, forgot to ph it down but hopefully it won't matter too much in soil, she's had a good stretch over the last day or 2.

Día 13 (06/05) Nos acercamos al final de la fase plántula. A ver si ahora empiezan a crecer de forma explosiva! He sido muy estricto con los riegos, dejando que el top hasta el nudillo se seque completamente! Día 14 (07/05) Hago una mejora en la tienda. Hasta ahora tenia intracción pasiva, pero ahora he instalado una turbina para que impulse aire fresco dentro de la tienda Día 15 (08/05) Con el calor que hace me sorprende que las macetas sigan aguantando la humedad, pero al hundir el nudillo sigue húmeda. Mañana haré un riego de nuevo Día 16 (09/05) Relleno el top con sustrato para compensar la compactación y dar mas soporte a las plantas Riego con 300 ml solo H20 - pH 6,2 Día 17 (10/05) Baño de sol de dos horas. Día 18 (11/05) Baño de sol de dos horas Día 19 (12/05) Riego 750 ml H2O + Regulator 0,15 ml/l + CaMg-Boost 0,25 ml/l + Startbooster 0,25 ml/l - pH 6.2 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

This week we will see the colas and buds really dense up. We are about 2 weeks away from harvest now. Next week I will start my flush.

Another great week for the girls, I have not seen a single deficiency, other than the odd yellowed leave under the canopy, typically due to lack of light. I will come through soon and do a defoliation of both tents to allow for more light to penetrate, and perhaps get a good push for some girthy colas 😋. I have lowered the temps a bit in the tents by controlling my lung room temps, and they are maxing out at 77 degrees and hitting a night time cool of about 70. I have also lowered the humidity to hover between 47 and 52 %. Hopefully will start to get some nice colours as they finish. As I mentioned before, when I water is when I like to get under the canopy and check the stems and leaves for anything that shouldn’t be… and typically once a week after watering I’ll wipe down the tent and sanitize any surface I can. There is no reason to get lazy, that’s how you get Pests, Mold, and PM. Happy Gardening 🇨🇦👊❤️

Die RP 43 hat in dieser Woche begonnen die ersten richtigen Stempel zu bilden 👀 Auch der Stretch war in dieser Woche sehr stark ausgebildet, sie hat sich fast verdoppelt 🌱🍀 Ich hoffe sie geht nicht mehr arg zu lange in die Höhe 😏 Ich habe zusätzlich in dieser Woche die untersten Triebe entfernt und sie zwar lichter geschnitten ✂️ Aufgrund der Blütenbildung habe ich begonnen den Blütedünger anzupassen und Big-Bud sowie B52 ergänzt.💦 Sie wird langsam hungrig und benötigt die Nährstoffe.

🇩🇪 Zum trocknen habe ich die Pflanze in drei Teile geteilt und sie leicht vorgetrimmt (Fanleafs ab) an Kleiderbügeln im Zelt aufgehangen. 13 Tage bei 62% Luftfeuchtigkeit und 23 Grad 🇺🇸 To dry, I divided the plant into three parts and lightly pre-trimmed it (removing fan leaves), then hung it on hangers in the tent. It dried for 13 days at 62% humidity and 23°C. 🇪🇸 Para secar, dividí la planta en tres partes y la pre-podé ligeramente (eliminando las hojas grandes), luego la colgué en perchas en la tienda. Se secó durante 13 días a 62% de humedad y 23°C.

Esta semana ya realice la poda. Se encuentra en estos momentos en etapa de secado dentro de una caja especial para secados de flores que uso siempre y nunca me falla. En humedo hay 195g y utilice una lampara led de 150w en todas sus etapas. Próximamente estaré publicando cuando este en curado.

~ WEDDING CHEESECAKE FAST FLOWER by FastBuds ~ Well fam, here we go again with another epic strain from FastBuds Fast Flowering stable. After having such tremendous success growing their Gorilla Cookies Fast Flower outdoors last year, I've decided to run another of their fast flowering strains outdoors this year... Wedding Cheesecake Fast Flower! The best description of this awesome cultivar comes directly from my friends at FastBuds which is as follows: "A delight for the mind and body: Wedding Cheesecake FF (Fast-Flowering) is a delicious strain that offers XXL yields in a 7-8 weeks flowering cycle. This terpene powerhouse produces loads and loads of mouth-watering vanilla-creamy-cookie-scented terps with hints of citrus and cinnamon. In addition to its delicious aromas, this variety grows very well in both indoor and outdoor setups, yielding up to 650 g/m2 without extra maintenance, making it the ideal strain for beginners as it allows you to maximize yields while minimizing work. This super-fast feminized photoperiod version offers upbeat and energetic yet deeply relaxing effects that are perfect for consumers looking for a heavily focused, motivating, and creative high. It’s the ideal strain for those needing that extra energy boost to start a busy day on the right foot. Wedding Cheesecake FF boasts chunky, large-sized buds with a thick layer of silvery-white resin and bright orange hairs scattered all over: that just screams ‘quality’. The lime-green flowers sparkle with an array of hues ranging from dark to light green, to magenta and purple, and reek of a delicious aroma that’ll remind you of fresh store-bought cheesecake. This is an XXL hybrid that grows extremely tall in height, reaching up to 3m with one huge main cola and multiple long side branches that can withstand enormous yields of up to 650 g/m2 with ease. Wedding Cheesecake FF is extremely resilient to the elements and can be grown in almost every climate as long as you’ve got the space and basics covered, making her an excellent choice for those looking for large-scale harvests with a quick turnaround time without much effort." ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Wedding Cheesecake FF indoors as our weather is still too cold to put her outside (nighttime temp's dipping regularly into the 30's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures stay above the mid 40's℉, at which point she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes, and then let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 5/3- Week Six is here and the Wedding Cheesecake Fast Flower by FastBuds is on a mission! Yesterday, I spread straw over the entire mound of soil and surrounding area to help keep the soil from drying out so fast and the watered with well water through a hose. 5/5- I'm noticing that something has taken a likening to the plant and is eating holes in her shade leaves. I went ahead and sprayed her down with Neem oil and will keep an eye out to try and determine what/who the culprit is. 5/7- Rain showers today handled the watering duties and all I did was to add some straw to a couple of bare spots on the mound. 5/9- I'm not seeing much in the way of any new damage to the leaves of the Wedding Cheesecake FF and I am continuing with the Neem oil preventative along with spreading some powdered cayenne pepper around the base of the plant to take care of any slugs that may think of trying a taste of cheesecake! There's another week gone by for this lady. The weather is getting better by the week and her growth is responding to it. The next weeks should be fun! Stay Blessed! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏