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@Terpyboyz
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So this grow came to a finshed but I had a. Few issues that’s held me up from finishing up the diary, but in due time I will reupload it all in order soon. But over all I will also update with a final weigh in of each strain produced so stay tuned 🤙🏻🔥
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In the end she went longer than expected but that's cool 😎 super dense buds . I really enjoyed this strain. Very nice smoke. Gromie's use the promo code GROWEED for a 15% discount on any Fast Buds purchase.
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@IamCy
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Here we are. Week 2. On the Day 8 video, I give my method on how I produce nearly zero larf and huge buds. It's easier than most think 😉
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Day 69 flowering: The Peyote Critical is dry and ready to trim for her harvest Tonight. She smells very fruit punchy and a sample bud nearly gave me a " whitey" as i overdid it with the dosage. lol. I have taken the Hulkberry out too for drying ( has her own diary) and also the RQS Critical( 60g dried bud). This made space to move the other ladies around and under the full working light. I took the Liberty haze out today at 69 Days from flower start ( not flip!). She was definitely ready to come out and her colours up close are so beautiful She is huge and has at least 30 decent bud sites. I struggled to get photos of her in such a small space so had to hang her just to get her all in a shot. Her colas are big too , hopefully she will topple my biggest yielded plant at over 10oz. The utopia has all been moved under the stronger bulb now so hopefully will finish this week better. They are very oily and smell of diesel oil and fruit. Not sure they will yield too much thanks to duff bulb but they look amazing and i cannot wait to try a bud of it. The Laughing buddha's are finishing up nicely now. Sadly the stunted one wont yield much at all but smells so pineapple. Her bug sis in growcaps soil is a monster like the liberty Haze so with only 1 week odd left. she is right under the 600w duff bulb close. Fingers crossed all will be done by next week and harvest reports can begin.
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Lots going down this week! I threw up a couple pictures today (day 74) I'll add more pictures and videos later on but I wanted to get this week started... Day 70; Nothing much to report except we got the new Optic 8+ 120°lens LED installed!! We got her put up around day 69. It's the 2nd optic weve bought and love these lights! The plants reacted immediately and we only started them at 80%veg brightness 20%bloom. Increasing 10% or so a day. I also built a cloning station so I can take cuts tomorrow just incase we fide a unicorn!🤞 Day 71; Officially on 12/12 light schedule now! I've decided to keep #2 in her 3gal pot. #1 gets the 10gallon smartpot. Both are doing terrific. I top dressed with .5 table spoons per gallon of soil on both plants using Papa's Perfect Poop. Made buy Michigan made mix m3 soil company. Its 5-10-2 using 100% organic fossilized bat guano Day 72: I defoliated both plants today. Fairly heavy but not extreme. I took all the larger fan leaves out that were blocking the lower bud sites. I also took clones today. 2 off each plant. 1 of each clone went into an ez-cloner that uses spraying water to root them. The other 2 cuts I put in a hydrofarm cloner that submerges the stems in aerated water until rooting. Day 73; The girls both bounced back and are praying beautifully after the defoliation. Both loved it! I can definitely see the start of new growth already on the lowers. I'm keeping the clones on 24hr light schedule. I know its addition stress but it's easier for me. Day 74: Literally nothing happened today. I sat and stared at them for maybe 2hrs but that's about it tho lol Day 75: A bit done today. I rearranged the tent hoping to give the girls a bit more leg room(you can see #2 in the picture is growing all funny because of space) I also watered #2 tonight. I'm finding the Choc Mint OG do not like much water. I also took them out of the tent for some glamour shots. You can really see what difference 7gallons of soil can make in terms of size. #1 is nearly a foot taller. Both are starting to stretch. Curious how much that is. Put #2 out in shades place outside to harden up Day 76: Not much going on. I watered number 1. Day 77: Big day today. 3 days ago I put #2 outdoors in shade to acclimate. I know I rushed that a bit. Should have let her harden up a bit more first, but I wanted her in the ground and growing roots while she has time. Its gonna be close but I figure all my other outdoor plants are just starting to flower so why not? My tent was a little crowded so being outside will give her the best chance possible! The Gear: 4x8 grow tent 2x Optic 8+ LEDs AC Infinity t6 fan 8,000 btu portable air conditioner 50pint dehumidifier Happy Frog Soil Mother nature 1 person dedicated to the plant
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@MrJoint
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✂️ Defoliation one more time and LST adjustment. 🥦We stay training. ✌️🎃Thank you for checking my cultivation.
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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Ya todos lo brazos estan dando su pelitos vamos bien y sin problema
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@Blakoby
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If anyone can help with the purple on the stems it would be greatly appreciated!
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@gr3g4l
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Esta semana es la última de las Mack&Crack, llevan ya varios riegos con agua sola, están mas que lavadas.
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Time flies, we have arrived in a flash at day 16, it seems like the other day that the first young ladies were appearing on September 1st. "Do you remember.....nanannana Septermber" She is Cosmic Noodles, a brand new strain from Zamnesia, so with little feedback but lots of expectations that we are here trying to make come true. In this case we are succeeding greatly, the plants are very pretty, one specimen is really gorgeous and super advanced for only being on day 16. The plant is at low/medium internodal distance and develops a very beautiful structure and leaves. We are very very very slightly under fertilization, I am trying to be perfect for the Zamnesia elves and I try to notice, whip myself and fix every mistake, even the smallest one. In this case we are a little under fertilization, in fact the plant is perfect, only that I went easy with the Grow to avoid over instead the Plagron tables, especially if created to measure, are very effective, follow them and you will not have problems. --- feeding program - As I mentioned I was shy about fertilizing and the seedlings are slightly, very slightly faded, let's learn to notice these small signs and with normal doses after two or three waterings we should be fine. You have to look at the plants guys. - Power Roots - 1ml/l - Alga Grow - 2 ml/l (instead of 4 ml/l I made this mistake) - Pure Zym - 1 ml/l - Sugar Royal - 1ml/l - Vita Race - 0.5 ml/l - The 100% Organic pack by Plagron can be found on Zamnesia at the link: https://www.zamnesia.io/it/11457-plagron-easy-pack-natural.html We are giving a lot of humidity with a humidifier and we oscillate between 65% and 70%. // Strain Description //cross of Alien OG and Rollercoaster Haze, Cosmic Noodles offers excellent yields and extraordinary aromas. This variety is very powerful, contains 25% THC and produces intense and long-lasting effects. Stimulating and relaxing at the same time, Cosmic Noodles causes an energizing cerebral effect that fades over time becoming soothing at a physical level. - Get a seed of this fantastic strain --- https://www.zamnesia.io/it/10777-zamnesia-seeds-cosmic-noodles.html - Soil and Fertilizers entirely organic --- https://plagron.com/en buy on www.zamnesia.io - Growbox and air system --- https://www.secretjardin.com/ - Light - Sp3000 - https://marshydro.eu/ - Music and sound --- I made my girls listen to 432hz frequencies and music from www.radionula.com - Z --- You can find these seeds, much more from the world of cannabis, mushrooms and an incredible series of accessories and gadgets on the reference site not only mine but of many growers ---- https://www.zamnesia.io
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@DanM1727
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Sorry about the shakiness of the video, first time with the new magnifier, will be better next time. Loving this plant.
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@4F1M6
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Stretch is done and flowers are piling on now! I treated with lost coast as a preventative. I kept the feed the same but it will be altered end of grow week. Canopy is getting hella full can't wait to watch these colas stack on. Until next update. Happy growing and stay lit fam.
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Day 45: The plants are growing strong, and looks healthy. I do not dare giving them any nutrients yet, but I still think I will give theme some soon
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A memorable harvest. The buds are all very high quality and cleaning them was easy as the pruning I did did not produce any popcorn buds. The flavor is among the most intense I've ever tried and it's a plant that I would recommend to everyone.
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@DrBud420
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13.06. Dos Si Dos 33# 1# Day 49# Dos Si Dos 33# 2# Day 49# I have nothing to write about these plants, they are progressing excellently, already tested strain, premium in every sense, 10/10 5 nights ago there was a strong storm, the morning after the storm when I came to the site, I found some plants crooked, some normal, but there were no broken ones, thank God, but they were very stressed and what you can see in the pictures appeared on the leaves, some leaves were crispy at the edges, but still green, mostly shoots before the newest ones. I haven't had this problem before, I researched a bit on the internet and came to the conclusion that the wind burned them, and I also turned to GW for an opinion, two characters confirmed my opinion. Two days later I noticed that the matter was getting worse and that it was spreading, which worried me, so I contacted GW again for an opinion. Some told me that it was mold, some that it was an infection, disease and so on, mostly guesswork, but no one specifically told me what was certain, so I decided on nim oil, and whatever it is, I guess the problem should be solved. According to some leaves, I would say that insects might be the problem, but I really don't know, I haven't had similar problems before. I regulate the ph of the plants, I still don't feed them, there is food in the ground for another week, except for the fact that I added cal-mag after that storm when I watered them. I want to say that the plants are certainly not locked, and the heat is not yet so high that this would happen from the same, the more the temperatures have dropped and now it is perfect. Since transplanting, I have watered the plants only 2 times. Yesterday morning they were topped for the second time, only the main branches, I will do the next topping of the side branches. Last night I sprayed the plants with neem oil and already today the problem seems to be going away, if I tripped at least it doesn't spread further, that's for sure. I didn't mix the oil very well, I didn't add any soap or anything like that because I wouldn't really spray the plants with any chemicals, and on some of the leaves on one or two plants there is that thick, brownish liquid, so I hope it won't hurt them, I noticed that today during the day, I couldn't see it at night. I still don't know what the problem is, but my guess is still that the wind burned them or some insects. Speaking of insects, I think I noticed thrips on one plant on the underside of the leaf, so in addition to the neem oil I already gave, I also ordered SMC, so I will spray that at least once a week while they are still young. Happy Growing and Stay High!!!
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Hello friends, everyone! I would like to inform you that this report is being held within the framework of the local growing championship in which the winners will appear in 4 categories .. Our girl will compete for the title in the Best CBD category! wish us good luck!