Just water:))))))

Time to flower so she is now the flower tent whit some pretty big sisters.

I have a huge smile on my face because I’m getting closer and closer each day to smoking my own cannabis. Never buying weed again. Still on one tent but by this time next year the plan is to have a veg room and a flower room. Anyways. This past week has been great for the plants. Taking water nicely. All plants flowering nicely from what I can tell. Nothing looks odd. No insect or bacteria issues. Knock on weed... hope I didn’t jinx myself. I’m adding a dehumidifier and should be in by next weeks post. From what I read is if they get too wet and moist the plants can develop mildew or mold and fungus and also it says that after a rain or watering you want the pores of the leaves to open back up and if it’s too wet they never open and stop producing. Sounds good to me. Also. Got a new phone. First one in a long while and I made a short vid.

Week 2 in the books. I trimmed more lowers to help shape the tops the way I like. Swapped ratios from veg to flower to start the flowering process. One more week until autopilot.

Week 8, and they are shooting right up, not had any problems at all with them, gunna lolly pop them soon and let them recover then prep them to change to flower soon @growerchoice @SHOGUN COCO A 4ml/L 160ml @SHOGUN COCO B 4ml/L. 160ml @SHOGUN ACTIVE BOOST 2ml/L. 40ml @SHOGUN CAL MAG 1ml/L 20ml @SHOGUN ZENZYM 2.5ml/L. 100ml @shogun KATANA ROOTS 0.2ML/L 4ML

She’s not even close to being done and she’s looking so incredible. The smell gets intense the second you touch her. Nice and thick stems with a close bud structure. She’s drinking about 2-3 gallons per day at this point. Not showing any signs of slowing down, but the buds are super dense to the touch.

12/13 Week 5 and a big week it will be With all the problems of bad seed, bad rock-wool, and fungus gnats (just to add insult to injury) we still got a grow. All have been treated and top dressed screw those bugs let them eat neem oil and mosquito bits. The big girls in pots are good but the smaller in solo cups were damaged found the source of the critters but may not be able to eliminate it landlord let the roof get a little too leaky, That's ok we got this now. Ok fun stuff: Octo and Plenty will be topped today, its time Pussy is hitting the lower height range where she can be put into Flower. She has hit 16 inches expecting a 100-150% stretch so really want 18" before moving her. Waiting on a harvest there first in a couple of days so should be perfect. Small guys took a big hit from the bugs flushed them with 2.5% h2o2 as a last hurrah for the gnats last night then gave ALL some recharge this AM to give them a bit of help. 12/14 Order of flipping, Pussy (FF7) Plenty (Grn Crk) Octo (LSD) this is based on best guess (guess it is) on stretch Prob dont want the Green Crack (150%) getting too large before moving her, know how that can go 😏 Do want the LSD (100%) pretty good size and the F7(125%) is just ready to go any day. yeah I know wild assed guesses Octo and Plenty are topped and look well, very bushy already Ah screw it moved Pussy into Flower it got crowded in veg and she is 17.75 inches, close enough Solitaire moves in to replace Pussy in the Medicinals... yes its a good one as well. Dont judge... we all did it! Pics Have four different feeds for this grow so will be only tracking Pussy from now on... love this grow Heh The veg tent big girls - 24ml/gal Grow A/B + 2ml/g kanga The veg tent small girls - 10ml/gal " " + 2ml/g kanga The veg tent seedlings - 2ml/L " " +0.5ml/L kanga The sick plants - 1ml/L " " +0.5ml/L kanga Wont change what Pussy gets till tomorrow as she is in for a very long day today. Yep love it Will be working on the roots a while given how those things messed with those. All plants getting some kangaroots affected plants will stay on a low dose, 0.5ml/L till they turn around. !2/16 We have a case of mass survival here folks, the small affected plants are living that should not still be here. Not sure what we will do with em but welcome to the zoo girls. Wife has even managed to turn around a FF2 I was sure was doomed. Yeah she is a keeper Counting chickens perhaps but looks so much better Still dealing with gnats but they no longer dangerous those at least we understand. Each cycle there are fewer CLONE DAY FOR PUSSY! Been looking forward to this have the propagator set up in its own tent we will take up to six clones from her as we may want up to 3 for the grow. Octopussy and Plenty will go into the Flowering tent tomorrow. Chew Mee growing again, looks better Solitaire just looks great Bambi needs transplanting - 2 gal she is sativa The sick germs are turning around finally have them covered to prevent any further nastiness though not think its an issue any longer. See what we get here 12/17 #HisHope has saved another, meet Dink an FFT-2 she will likely stay in a one gallon pot we will see what she does This is particularly notable as she is in rock-wool was like the kiss of death The Medicinals are together in the Flowering tent Plenty and Octo will stay on their veg feed a bit longer. Bloom nuets introduced to Pussy she is stretching very slightly Germs are improving the Gold Leaf and FF8 doing best so far. 12/18 ILGM standing by their word and making good on the Gold Leaf, new pack on its way. Hope one of these will come on up. Clone day for Octopussy and Plenty six from each 12/19 Adjusted feed for the medicinals increasing bloom decreasing grow

Girls are doing well....seed looking like she earning her name..have some cutting off her n am please with the first week of flower....one got super stress n she dont even look like it jus watchin to see how things look later

todo va bien, olores fuertes e intensos y dulces, en general poco falta excepto dos plantas a las que todavía regaré algunas con fertilizantes 🍁🍁🍁🍁💪👽👌🍁🍁🍁🍁

Day 56 This plant looks so small, because i High stressed them, im still giving them a Chance but they are so smalltalk that they need weeks to recover , and if in 2 weeks the plant dont Show any signs of getting flowery then i will cut her. Some week or 2 stopped, because of The Training.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Last week of nutrients finished, plant will now be flushed for 2 weeks. Just had a harvest from another seed of this plant and we've decided to call the strain "Monkey Magic", as a few puffs will summon a cloud which whisks you away into the sky! 😀

Pre flowers 🌸

buds getting bigger

Harvest time 🤩💥 I am very pleased with the result, I'll let them dry slowly,and report about taste and weight.Stay tuned for more updates!

Easy to grow , no real problems . Was ready in 8 weeks but could have gone another at least . Next time I will flower for longer to see if there's much difference in that last week to ten days. You can find these beans at : Zamnesia.com 20%off code : ZAMMIGD2023

Glad to have gotten what I did. Small harvest. But it wasn't about that for me. I done a huge number of crops to hone in what traits I like. And now I pick with much more by looking at the genes and what went into making it.

preflower on Day 33