25.06 FD50 Nutrients: 14ml Biocanna Flores 2ml Biocanna Rhizotonic 10ml Bio Boost 29.06 FD54 Nutrients: 12ml Bio Boost

Day 120, 9/5, Day 50 of flowering, full week always one week back: Net down and holding in support. The discoloration is starting to spread and I'm still unsure exactly what it is or if there's time to address it at this point. I've got the lights pulled up just bit more, just over 17" to the canopy at the closest spot. Humidity and Temp have been goldilocks as far as I can tell. PPM was 1100 and 6.38pH on the last runoff with small amounts of Molasses, Senizyme, and a tiny bit of CalMag. Bag is 60% FFOF, 30% Coir, 10% additional Perlite. Have been using FF trio as my base at half strength, only other additions have been the occasional Senizyme and some B-52 back in veg. Day 121: Posting a video of entire plant. Having to get a bit creative to keep this lady up. Some more twist ties and a tie up. Day 122: Will feed tonight. Planning a clean feed with a slightly high pH to bring closer to 6.5pH. Fed: 6.8pH, 6L, 71F, 135ppm - Runoff 6.69, 545ppm. Day 123: Going to spend some time tonight trying to get a view of the trichomes without the glare of the LED on, seems I might get a better idea of what's going on. My wife took these pictures in the dark with flash only this morning. Day 124: Well, I think I'm considerably farther along than I initially thought. I think I'm harvesting this weekend? I've at least 60-70% cloudy, and several amber as seen in today's pictures. These are likely the best micro shots I can get. I have a handheld magnifying glass that's hard AF to keep lined up on my phone camera, especially with my shaky hands. On the other hand, these are pretty decent zoom pics, LG V60, so here's some free advertising for them I guess. Considerable yellowing of the leaves seemingly over night. Day 125, Thursday 9/10, Day 55 of flowering: So, I've decided we won't be using the Flawless Finish. I would have, had I another week to water again. Just worried about adding anything as this is my last watering. On my previous clean feed I had a run off in the low 500s, so I'm just going to 'flush' again with plain Ph'd water. Lights on thru Friday, if everything looks good, lights off all day Saturday. Sunday: harvest, trim, and dry! Night 125: So after fretting a bit much over the past two days, I called the guy at my local grow (and home brew!) shop. He advised 50/50 one way or the other on the Flawless, but of it was him he's pass if he was harvest Sunday, as I plan. Watered with plain ol' pH'd water, 6.5 input. The first gallon was warmer ~75F and run off was 6.3pH/1300ppm, second gallon at 70F, 6.5pH/900ppm. Assume the higher ppm is from the higher temp in water for the first gallon, which was done on purpose. Tomorrow is the last day of lights. Day 126: will attempt to get some good trichome shots tonight Going to confirm she's cloudy and amber all over then shut the lights off for 24hrs. Day 127: Lights out! In the dark til tomorrow. While I couldn't get any good shots, I would say bud to bud my trichomes are 80% cloudy, 10% glassy and 10% amber. There are a few trichomes I was able to focus on for a second that seemed reddish or purple: no idea what that means. My wife was able to sneak into the tent and get some Trichomes pictures. See the red & amber! Day 128, Pre-Harvest Sneak Peek & Pics: Wildly underestimated the amount of time this would take. We started at 3pm, and by 10pm we had only done about half the plant, maybe a little more. Granted we got up for small walks around the block every hour and stopped to make & eat dinner at some point, but I would say we did a solid 4-4.5 hours of trimming. We only clipped the arms of what we trimmed, so the rest is still in the dark tent. Hopefully we finish tonight! We weighed only the largest buds we've trimmed so far and it came out to 140grams wet. Day 130: Welp, didn't finish trimming til this morning. Worth it though! With some already drying for 2 days and some newer, the weight is likely hard to gauge at this point. Looks like the smalls are mostly dry already and weight in at 16gr, the slightly larger grade buds weighed in at 32gr, and the largest buds weighed in at 126gr now. We tried our hand at dry ice hash for all the trim (77gr wet) and got 2gr out of the 73 screen, and 10gr out of the 220 screen! Picture posted of product. See you all in 2 weeks for the harvest post!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

i have no more AN 3 Part i use now the Sensi Bloom A + B

Day 2. Training... Incl topping/FIM to increase nodes

Everything is starting to fill out. 2 are really starting to get nice colas. 2 are beginning to fill in nicely and the little midget has finally started to form buds. Probably end up moving her outside to finish up, being she's lagging behind. She's starting to show potential and may end up being a little bud bush...lol. They are all definitely going to have spaced out finish dates.

Defoliated today at day 21, I think I vegged a little too long as they got a lot bigger than anticipated. Now I know how this strain stretches I can judge my veg time a lot better next round.

Welcome to my Ðivine ØĠ Ķush diary. In this diary: Seeds: sponsored by Ðivine Șeeds Media: Promix HP Nutrients: Advanced Nutrients, Diablo Nutrients. Light and Weather: Şun☀️and Mother Earth.🌎 ___________________________ Feeding: Tue 18Jun: 13L water not pH'd Wed 19Jun: 10L water not pH'd Thu 20Jun: 6L nutrients pH'd 6.5 Sat 22Jun: 6L water not pH'd ___________________________ We went through a heatwave from Monday June 17th to Saturday June 22nd with temperatures of 34°Celsius with humid factor of 44°Celsius girls got thirsty and finally it poured rain from Saturday afternoon to Monday morning witch was more than welcome for the girls and our garden. Temps are back to normal now🌤️ Kush kush fell on Wednesday due of wind gusts, made an anti-tilting device🤓😂seems to work so far... we'll see when branches get heavier with the buds weights😅 ___________________________ Thanks for stopping by, likes and comments are appreciated!👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

Tous ce passe bien merci à tous pour votre soutien .

This girl is coming on really nicely starting to see a bit more to her now. I have up’d the big bud nutrients this week so hopefully it “puts the big in your buds” increasing both their size and quality It has been about two weeks now since I nearly snapped a branch clean off. I strapped and propped the snapped branch to the main stem as soon as it happened but when I checked on it the next day the strap had slip. I did worry as I wasn’t sure how long it had been apart but I re-strapped it and hoped for the best. So far the branch still looks healthy and I haven’t noticed any issues with it at all. The branch looks as if it’s healing really well so hopefully she pulls through. Happy Growing 🌱

The first thing I did was disinfect everything and put it in the shower. Then I calmly rewired the tent and filled the pots. 20% clay balls at the bottom as drainage. On top 50% Biobizz All- and 50% Biobizz Lightmix. I mixed the whole thing well beforehand. 3 seeds came out great, one is currently fighting through and I don't think anything will happen to the last. I planted them all today. Let's see if the last one can still make it.

Привет друзья, всем тем, кто следит за мной. Вновь обновляю свои дневники. Если вы следите за мной, то знаете, что на протяжение всей прошлой недели я брал клоны от своих растений. Вы можете следить за их развитием в соседнем моём дневнике. После того как я взял достаточное количество клонов, я сломал привычку растений выращивать один центральный стебель. Теперь я дам им немного покоя и буду наблюдать, как их внешний вид восстановиться после жёстких тренировок. Я даю растением небольшое питание, в основном из витаминов, гуминовых кислот и бактерий. Когда мои 50 клонов будут готовы к пересадке, я переставлю материнские растения в палатку поменьше и буду дальше экспериментировать над ними. Если вам интересно следить за приключениями травки в России, подписывайтесь на мои дневники

Bellissime genetiche

Bienvenidos la semana #3! 😁 Día #18. Crystal Candy XL Auto ha empezado a crecer de forma espectacular! Northern Lights auto ha mostrado las primeras preflores y también están empezando a crecer muy rápidamente Royal Bluematic ha pasado por el bloqueo de nutrientes por el ph alto en el agua de riego (es la única strain que la ha manifestado). Se ha regado con agua con ph ajustado para controlar el bloqueo. Orion F1 están yendo más lento que el resto...  Día #19. Crystal Candy XL Auto y Northern Lights han empezado una carrera hacia el cielo Se decide empezar con LST en todas las variedades (ver fotos)  Es primera vez con LST, así que espero hacerlo bien! Pero supongo tendré una curva de aprendizaje o ensayo y error Día #20. Crystal Candy XL Auto se muestra muy sedienta. Sus compañeras siguen con 100 ml de agua desde hace unos 4 días, y ella necesita 250 ml de agua cada 3 días (también es la más rapida). Se ha regado Crystal Candy XL Auto con 250 ml de agua más 2 ml/l de Bio-Grow de Biobizz Es necesario ajustar el LST a diario  Día #21 Se hace un riego con 100 ml a todas las plantas (excepto Crystal Candy XL que se regó día anterior). LST es exigente en su revisión diaria pero divertido! 😃 Día #22. El tiempo se está volviendo soleado y hacemos el primer baño de sol.  Crystal Candy XL Auto está respondiendo muy bien al LST, necesita ajustes a diario Royal Bluematic tbm responde bien al LST. Las Orion F1 están yendo lentas... No sé si cogerán velocidad la próxima semana He detectado que Northern Lights tiene una pequeña plaga de minadores de las hojas (solo una hoja afectada). Para atacar la plaga y por precaucion, se hace aplicación foliar a todas las plantas de jabón potásico y aceite de neem.  Día #23. Descubro que he quemado algunas hojas tras la aplicación foliar de ayer y meter demasiado rápido bajo la lampara... Salimos al exterior! 😂 La idea es dejarlas en exterior el resto del cultivo, a no ser que vuelvan las lluvias (espero que no! 🙏) Pequeños ajustes de LST  Gracias por leerme!  😄

Comenzamos su 2°semana aportando los nutrientes principales Deeper Underground de Top Crop para su sistema radicular añadiendo 1ml/l al agua de pH6 Día 9: La pequeña ya alcanzó los 5cm y sigue creciendo bastante bien. Ahora lo importante es que cree un sistema radicular en perfectas condiciones.

A great week. Included a diagram so you can better tell which plant is which. I will be changing that up come flower to better fit the plants so I'll label them later as well. Gonna flip to flower I believe to try and save space. Gonna be tight but wasn't planning that this time. I got some BT var israelensis or something close to that for the fungus gnats thanks to @resimax and will test that. Lastly, you might catch a glance at the upcoming diary...

Well, six months later the indoor side of the grow has finished. A final timelapse is up, capturing the last week of the plants' lives. I'm quite happy with this grow, aside from some foxtailing at the end all went extremely well. UKBS 1 with the frosty, insane smelling quality flower and Carl 1 with giant, luscious buds that smell very fruity. I'll post a harvest week once they're trimmed up, and of course review the strains once they're smokeable. I'm quite ecstatic about the weight and quality of this grow. This diary will continue, as the outdoor plants are still going strong. (of course) - However there will be no more EOS t5 pics until they head into flower in a couple weeks to a month here. updates come throughout the week, as per usual :p Thanks for coming.