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@Mazgoth
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This strain is getting fatter every day and the trichomes multiply and become more amber.
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@Theia
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This plant is a beast.. she is massive and well into her stretch. Loads of bud sites and great spacing between nodes. I had to supercrop her as she was pushing twice the height of all othe girls in the room. She took it well and is fully recovered and just great. Love this plant. Very odd smell quite disgusting ATM tbh.. I started giving this girl a light misting with some fishmix and activera for a few days as she was looking like she was not getting enough food. 5 days on she is looking good Thanks for stopping by. Happy growing all🌿🌱 Stay safe😷😷😷
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Start of week 7 and all plants are looking great. Added the net to hold them later but I may take it out. Ran into a slight issue…my plants got so tall that I was not able to provide enough PPDF to the lower stems (I need a bigger tent and better lighting…in time). As a result a caused light burn on the top leaves. Additionally, early in my grow I was using Mass Pro. That alone with amendments and I foliaged early with Foop. I believe the Foop and Mass Pro were too much for these plants to handle. Still rocking though…
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@ThaKass
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Well I must be doing things right, for an auto this girl is doing really well. I’m still learning but will be growing more Bruce Banner. I’d soon rather have sativa but takes so much longer to grow. She takes all the nutrients I give and drinking more water this week, had to top her up a few times. PH is 6.4 TDS is right around 1000 Temp is 20c Humidity is 60-70
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@Dingle
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The trichomes towards the end of this week had started to go slightly cloudy so I decided not to feed and to start the flushing process instead. I flushed the medium with a couple of litres of water per plant until I got a good amount of run off. I think I may have not back off the nuets enough on the few last feeds as the majority of the leafs on all 4 plants are still quiet dark green in colour with no yellowing. I did a good amount of defoliation at the start of week to try and get as much light to the lower areas as possible. The buds are still developing ok I think they feel dense, they won’t be massive buds but I am hopeful for a decent yield overall. The smell is strong but the carbon filter is working great. Happy growing so far 🤞🏻
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@Njaak
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Microscope shows the telltale amber in some of the trichomes :). Did a full system clean with H2O2, long rinse, and the new solution is just pH balanced water and FloraKleen. Some crispy leaves at the moment but I'm not worried. Harvest is coming soon! Next post will be a harvest post and early smoke report 🙃
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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So welcome to Week 9 of the Sweet Seeds Gorilla Girl XL Autoflowering grow! Unfortunately after two years of due diligence- I was finally infected with Covid 19 so I have gotten really behind with this diary. In Week 7, I switched from the Grow to the Bloom fertilizer at a rate of 2.5 mls per gallon. I am still watering every 4th day at 3 litres per plant or 0.75 of a litre per day per plant. This strain seems particularly hungry/greedy so by the end of Week 7, I’ve decided to fertilize with every watering instead of every other. I’m not increasing the dosage of the fertilizer but the frequency in which it’s being given. Other than that the plants are doing fine through Weeks 7, 8 & 9 with the buds fattening up nicely, loads of wet to the touch resin and a super strong smell emanating from the tent even when it’s closed! That’s it for the past three weeks, Covid sucks and thank goodness harvest is just around the corner! Apologies for not offering up any further "mom’s tip of the week" but I’m just finishing up this diary for kicks at this point. Thanks.
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🌳🌳🌳🌳🌳
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**Encontrarás la traducción a español al final de la descripción** From/Desde: 01/03/19 || To/Hasta: 07/03/19 From day/Desde día: 8 || To day/Hasta día: 14 You can find the Gorillas Diary here: ** Podéis encontrar el diario de las Gorilla aquí:** https://growdiaries.woodroom.tel/diaries/25675-makingmoney-with-gorilla-mm-vs-gorilla -----IMAGES & VIDEOS----- Video 1: Timelaspe video covering 5 days, from Monday 4th night to Sunday 9th afternoon/night. One second Blackouts on video represents night times. I'm sorry for the dancing of colours specially first days, i will try to do it better next time. The background song is from the old awesome album "Deep Forest" of Deep Forest "Sweel Lullably" song (I think i'm going to use this song for almost all timelapse plant growing videos). -----WEEK SUMMARY----- I'm sorry about the delay updating this week, i traveled to Venice at the end of this week and that's why it's late and i have very few or non photos of the next week. I wish the almost 5 days timelapse video can cover the lack of images for the next week. As you can see the Moneys are growing very equitative, the one that looked small last week is now of the same size as the other one. among the 4 plants shown in the group photos, only one has some kind of problem, and is not a Money maker :). As soon as i could perform a man-lining on any of the moneys i will. LST STRUCTURE: To prevent breaking or making holes in the pot i designed this LST structure in blender and I printed it in 3D. They fit perfectly in the 8l RootPoch pots, so at time i didn't fixed them to the pots. If i see any of the plants lifting the structure i will just add weigh over it. -----WATERING CALENDAR----- 06/03/19 - 1.250 ml with all week nutrients and 1 CO2 tablet over the soil @ 0.4~0.5 E.C. PH5.5 (I'm going to travel to Venice till Sunday so i watered well) *****ESPAÑOL***** -----IMÁGENES Y VÍDEOS----- Vídeo 1: Vídeo TimeLapse que cubre 5 días, desde el lunes 4 por la noche hasta el domingo 9 por la tarde/noche. Los negros de 1 segundo que aparecen en el vídeo indican las noches. Siento mucho las variaciones de color entre los días, especialmente los primeros, la siguente vez intentare tener mejor color y más estable. La canción que suena de fondo es de un album antiguo maravilloso llamado "Deep Forest" de Deep Forest, la canción es "Sweet Lullably" y creo que la voy a usar en todos los timelapses de cultivo). -----SUMARIO SEMANAL----- Siento el retraso en el update de esta semana, me pilló el fin de la semana con un viaje que he hecho a Venecia y por esta razón llega tarde esta semana y de la siguiente tengo pocas fotos, espero cubrirlo bien con el vídeo timelapse que he hecho de casi 6 días Como se puede apreciar, las Moneys están creciendo equitativamente, la que parecía pequeña la semana pasada, se ha puesto a la par con la otra. De entre las 4 plantas que aparecen en las fotos de grupo, sólo una tiene algún tipo de problema, y no es una de las Money. Tan pronto como pueda man-lining en una de las Moneys se lo haré, creo que va a ser en semana 4. ESTRUCTURA LST: Para no agujerear los tiestos he diseñado esta estructura para LST con blender y la he impreso en 3D. Se ajusta a la perfección a los RootPoch de 8 Litros pro lo que de momento no la he fijado al tieso ni suelo mediante nada, si veo que alguna de las plantas la levanta, simplemente pondré peso sobre la estructura. -----CALENDARIO DE RIEGO----- 06/03/19 - 1.250 ml con todos los nutrientes semanales y una pastilla de CO2 sobre la tierra @ 0.4~0.5 E.C. PH5.5 (Como voy a viajar unos días he regado bien)
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Clipped all major fan leaves and started flushing yesterday with only 4ml/L of flawless finish. She's still very coated in resin and her smell is still very strong. No purple colors yet but she has moved from a very like green color to a more golden hue. I plan on watering/flushing every other day until next week when he reaches her 56 day mark. I may leave her a bit longer based of trichome development so we will see and until next week.............. #HappyGrowing
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Extremely disappointed with the yield but that is my mistake by keeping this 100% indica from getting bushier and expecting it to stretch more. Definitely many lessons learned this grow. Going to add weekly flushes with beneficials in my next grow schedule from now on.
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@Theia
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Hello growmigos I don't think she is gonna finish off. The weather has really come in it's cold and wet and I think I'm either going to have a Hermie bush of pollinated hash material or I will have a putrid soggy mess of rot.. Maybe I am being too pessimistic but that's how itt feels. I put her in way too late but such is life. The good news is she smells divine filling my garden with a kinda White widow citronella smell. Frost is not an issue either as she is stacking piles of this too. I have noticed an ant best nearby tried to use my lady to farm some aphids.. but they are not foliar with this species of plant and her resin so now I have hundreds of ant corpses stuck to the frost..🤣🤣 She will need a bath. That's all really will just keep an eye and if she looks like she's going south I will just chop her and bubble what we get. Thanks for stopping by. Stay safe 😷😷 Grow well 🌿🌱🌼
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@JohnWeed
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Hello guys, I had less time to updated these time However the girl are in bloom and are doing well I start this week 3ml/L biobloom and it is very good the buds start to inflate well
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Que pasa familia, vamos con la segunda semana de floracion de estas Lemonpaya feminizadas de fastbuds. Vamos al lío, de las 3 plantas, me quede con 2 por espacio, siempre pongo alguna semilla de más por si no abriese alguna por no perder ese hueco del indoor. También se trasplantaron a su maceta definitiva, en este caso de 7 litros que además provocó un shock que también solucione. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. Hasta aquí todo, Buenos humos 💨💨💨
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Week 7 of Veg for these beautiful Apple Betty ladies! It was a stressful week for the girls but they made it❤️ Day 44 - I decided the girls had outgrown their 2 gallon nursery pots and were ready to move on. After a quick cleanup of the stems close to the base, they were ready to go. I like to keep my soil in a Rubbermaid bin, either with a fan lightly blowing, or the exhaust line from my tent blowing at it. Since I started doing this, I have not had any gnats or pests in my grows, of course with regular cleanings and IPM…my mix is Promix HP with about 20% worm castings added to it. This time around, I decided to use some Dynomyco to increase the mycorrhiza count of my Promix HP. This will increase root development by adding all kinds of interconnected webbing and allow for better uptake of nutrients overall. Within an hour or so all of the ladies were transplanted ❤️ x2 in 7 gallon fabric pots, and 1 in a 5 gallon fabric pot ( the 5 gallon will finish in my 2x4 tent) Once the girls bounce back and take hold of their new forever pot, I will be setting up my custom “floating” SCROG net and allowing them some time to get bent and tucked all nice snug before I flip to a flower schedule. Happy Gardening ❤️🇨🇦🌱😎
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@Lazuli
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As u can see this plant is super bushy, but inside there are many rockhard buds full of frost
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Great week 4 for my 2 lovely Papaya Sherbet