Germination date 🌰 14/03/2021 Day 77 🌱 30/05/2021 Strain 🍁 Peyote wi-fi, Seedsman Nutrients 💉 Advanced nutrients PH perfect sensi grow A+B (veg) PH perfect sensi bloom A+b (flower) B-52 (through veg until week2 of flower) Voodoo juice (🖕🏻) Tarantula (🖕🏻) Piranha (🖕🏻) Sensizym (all the way through) Rhino skin (🖕🏻) add first leave for an hour Big bud coco (week2+ of flower Bud xfactor (🖕🏻) Nirvana (🖕🏻) Bud igniter (first 2weeks of flower) Overdrive (last 2weeks of flower) Flawless finish (flush week) RockHoldings Rockresinator(week2+ of flower) Vitalink calmag Set Up ⛺ amazon special 1.2m x1.2m 💡 spiderfarmer sf4000 📤📥 AC infinity 6inch 💧 10lt dehumidifier Notes🗒️✏️ Nothing to report from last week. Seems to be taking a little while to bud but no complaints as it smells 👃 f**king amazing. Been doing autos that long I forget how good photoperiods smelt. I get why people do autos but personally from now I will never do them again. Next grow will be the one to look out for 👀 Sin city's WHITE NIGHTMARE x Biscotti Sundae (Frosted Biscotti) Sin city's WHITE NIGHTMARE x Purple Yuzu (Yuzu Sorbet) Ethos genetics Grape God x Mandarin Sunset (Banana Hammock Rbx1) Depending on which one comes out better (purple matcha) / (peyote wifi) that will take the 4th spot in the tent for the next grow 🌱 Happy growing everyone 🌱❤️

Hallo zusammen 🤙. Das war es jetzt für sie wir sehen uns in 3 Wochen für den Erntebericht 🤙

Moved them both back indoors into the tiny tent under the Viparspectras..set timer to go 12/12.. I really need these bitches to flower...seems a lot like they were mislabeled... Finally....2 months in and pistils are popping after a few days of 12/12.... #notautosafterall 👉 Check out these new XS1000 lights on Amazon: https://amzn.to/3ttb2j9 👉 Get 10% OFF using this coupon code: lt10gdvip

This time I am growing Sativa strain that is Mexican Airlines from FastBuds and some cool hybrid Wedding Cheesecake from the same breeder. I started with 7 seeds. Mexican Airlines: 4 out of 5 germinated successfully. Wedding Cheesecake: 2 out of 2 germinated successfully. Currently I have 6 sprouts. Will keep 3 strongest of the first week of vegetation stage.

Zkittlez auto

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to Auto Alaskan Purple Grow by PoshGrow! 🍀 Week #5 2020 August 20th - 27th. General Info: When planted: 2020 July 23th. Week: 5 Days: 28 - 35 Last Update Day: 2020 August 27th. Plants: 5 Alaskan Purple Auto. Equipment: Tent: MarsHydro 1mx1mx2m or 39"x39"x72". Light: HLG 260w V2 Rspec QB Kit. Exhaust: 4" 322 CFM fan + Viper Carbon Filter. Intake: 4" 100 CFM Inline Fan. Oscillating Fan: Lower: 4" Ram Fan. Upper: 9" Voxon Box Fan. Humidifier: Taotronics TT-AH001. Dehumidifier: Pavlit MD750. Soil: NPK soil 40% Compost, 50% Peat Moss, 10% Agroperlite. Pot: 7 gallon Fabric Pot x 5pcs. Nutriens: Fox Farm Trio. PH Correcton: Chemoform pH-Minus Granulat. PH Pen: Cheap Chinese one, I callibrate it every time I use it. Water pump: Digital microscope: cheap Chinese USB X4, 1600X. Comment:

The seeds popped and are looking good in the humidity dome. I didint use rockwool. I wrapped a paper towel around the tap root and wicked it to the water

Defoled and tied down and she’s pumping now , another week of veg keeping up the defol and spreading her out before flipping. I’ll raise the lights this week to get a bit more vertical growth .

Still dealing with a little clawing... Two straight waterings with only pHed water (~1.75 G each time). No longer feeding any veg nutes so hoping things improve. Photos/video taken 105 days after breaking soil, day 28 of flower.

BLUE CUSH 35 Days from sprout. Light training. Leaf tucking and pony tailing. Pony tailing seems to help with the side branching. She is genetically a small plant I believe, but she might be smaller then expected.. Possibly due to lower RH early on. Otherwise she seems to be doing ok. Attempted a layer of clay pellets after soaking in PH'd water for 16 hours, to combat the gnats from reaching the soil. I feel like sand may have been a better choice and removed the clay before feeding again. There are so few gnats that its not much of an issue, but I'd like to eliminate the threat before flowering really kicks in.

🌸 Week 3 Flower — Bud stacking begins! 🌸 This week was a turning point — stretch finally slowed down and the plants are now fully focusing on bud formation. The tent started getting really humid due to the extra leaf mass and transpiration 🌿💦. That’s when I realized: a dehumidifier is a must. Without it, RH would climb over 70%, which is dangerous at this stage. Temperatures are still steady (27°C day / 20°C night), but now I’m working on dropping humidity to ~55–60% for safer flowering. Nutrient-wise, I reduced nitrogen even more, focusing on PK boosters and bloom nutrients. Bud sites are stacking nicely, pistils are shooting everywhere 🧡, and the first serious trichome production has started ✨. Mistakes from earlier (too much IR, uneven light distribution) are still visible on some plants, but overall the crop is healthy and vigorous. Next week should be the start of real frost explosion ❄️.

🍪🍬⛽️

I spend less time with them my friend usually look after them. She went on holiday so I will be with them lucky ones. They are okay big plants flowering nicely I constantly removing old yellow leafs. All the same they do well :).

I cleaned out the reservoir and switched from Veg to Bloom nutrients on day 36. My 35% Hydrogen Peroxide arrived, and that definitely makes a difference with keeping the reservoir cleaner longer, although I am still cleaning it maybe every 10 days or so, just a quick 2 min scrub with bleach/water, and then i run it through the pump and lines as well, then run plain water through it before hooking it all back up. The ONLY water I have used from my well this grow has been to clean my reservoir, everything else has come from my dehumidifer. I am now using some Cal Mag, Maxi Bloom, AND some Liquid Kool Bloom. I am running an EC of about 1450. My runoff from Coco is coming in around 1490 EC. Not seeing any issues with the ladies, just worried I may have waited a little too long to switch from Veg to Bloom Nutes, although I was adding about 25% Bloom for the past week or so. I guess we will see here soon if she gets some bigger flowers. Thanks for checking me out, and Blaze On!

Todos los días actualización nueva, pendientes !!

Can't tell I was happy with the outcome but I think this is more a indoor type.lots of problems with bugs and to hot weather.they did not Heald as fast as other autos..but still can't wait to smoke..

Day 36. One more heavy training session. Girls drink every day a liter at least, have to water them every 2-3 days and its good !! They got second wave of food , lost almost all oldest fan leaves and i think ill drop my plank to 80 g and will flower them next week. Still never cracked is it better to clear bottoms before flip or with first wave of defoliation during flower. This time i'll lolipop them in flower. In middle of last week found some mold on one leave - no waiting and hesitation - Biobizz LeafCoat applied ! Now can't find even smallest issue in them , pure perfectness !!! Still think 10 girls is too much for my setup to bush them out, but i just can't throw away any plant ;)))) Damn - lesson for life !! Leave branches alone after watering !!! Itchy fingers and smallest plant have only 7 proper branches left, thought to move one a bit ... ;))) Girls respond to training and food amazingly - late eves video is a proof ! Day 41. They just fly, thickness of branches is just amazing, leaves regrow faster than i pull them ;))) Today all girls will get bottoms cleaned and heavily defoliated, all tops will be HST'ed and i have power cut next tuesday, so monday - lights out for 24 hours of darkness, wednesday - flower starts ! Had to reposition them and bring in one more drain tray. Attack scheme still 2-3-3-2 ;))) All Blue Dreams hated it, loads of supercroping, one stem split, but it looks like all is finalised for flowering ! Girls got Great White Mycorrhizae tea. Next pure water, later Molasses and only then updated feed, Root Juice out, Bloom and Heaven 2x , all plans and changes ... Happy Growing !