Hi, topman! This is 70 days from changed light 12/12. Next reports week well be with KiT2 and 77 day. Day of Harvest! See you and thank you for your time.

This gurl is done! She smells and feels sticky buds are not huge how I wanted but I take into consideration I use very little fertilizers and she started turning yellow and leaves dying!

Amazing told this run 110 grams of bud and 80 grams of shake. An amazing pungent fruity citrus cream cheese aroma eminates out of my cure vault. The high has been described by my friends as a creative uplifting high followed by intense couch lock. The grow itself was a breeze. The only real issues i had in the hydro grow was that i needed to to add cal mag because it was growing so fast and i was using distilled water. I basically did not train my plants other than defoliating to make space for air flow and light penetration. I would recommend this for any first time hydro grower that has a decent amount of space.

🍀06/07 - Empieza su sexta semana en etapa de floración. 🍀Se encuentra perfecta, se desarrollo muy rapido. 🍀Se encuentra llena de tricomas por todos lados, es increíble lo nevada que esta. 🍀En tres semanas aprox ya tengo pensando podarla, por el momento viene todo bien. 🍀Algunas hojas que se encuentran abajo de toda la planta se encuentran un poco amarillas, eso es porque tuve q cambiar de lado los paneles de led porque creció demasiado alto, los próximos cultivos voy a estar usando la red de scrog. 🍀En estos dias seguire subiendo imaganes de como se encuentra. 🍀😶‍🌫️🇦🇷Podes seguirme en Insta gram como @bruweed_arg🍀😶‍🌫️🇦🇷

vid is from day 22 picture is day 26

Growing beautifully, not much to add really,.. roll on next week

Will report back with a smoke report, but this thing smells incredible and budded extremely well. Didn't have much of any issues with pests. Got a BIG 4' plant from only super soil in a 5-gallon fabric pot. Tons of resin. Very impressed with these automatic genetics from Pride of the Lion Seeds... and I'll definitely be growing another one of them in the spring.

WASSSAAAAAA

Haven't had much time to take care of the plants, just fed once with 2ml/l of Bachumus Evolution Floración and that's it. The leaves continue to yellow. They're starting to smell a lot now, will probably have to use the filter in the next couple of weeks.

First signs of flower on the big one ✨ also I lowered and rearranged lights for a better Ppfd I should have started before but I also added some Voodoo Juice, hopefully it will help with the smaller ones 🤞🏻

This is my first grow ever I forgot to add more photos of other plants on next update I will have all photos!! I am doing everything organic, I am using compost soil from my cattle and other top soil I have been composting for awhile now. I am also using earth worm castings… I also have earthworms in the pot with the plants.. seem like all plants have ton of white hairs coming out now I don’t know what that means but I am learning as I go!!

The photos speak for themselves, I can only add that it was a pleasure ...

estas pequeñas sorprendieron con la prdoduccion de resina que dieron 22 gramos aprox y 19 cada planta

It's a nice change of pace to have these warmer temperatures, even if it's not quite spring yet. Low 40s during the day is a lot more comfortable than what we've been dealing with lately. Hopefully, this is a sign that spring's around the corner. My Blackberry Moon Rocks are growing like crazy! Most of the leaves are a nice, deep green, but I noticed a few on one plant were a little yellow. I did some research and it sounds like they need some extra nutrients, so I'll be adding those in on the next watering. I can't wait to see how much bigger and bushier these beauties get.

4/15/24 - 4/21/24 ----------------------------------- 4/2 - Seed popped ----------------------------------- 3 Gallon Fabric Pot 50% Cana-Coco 25% Vermiculite 25% Perlite ------------------------- Tent: 2’x4’x5’ GA Tent 600D 1 Gal Humidifier 1x 6” Clip Fan 1x 28” Oscillating Fan 1x 4” Carbon Filter with 2x 200 CFM ------------------------------------------- 3 Gallon Tall Fabric Pots Spider Farmer SF1000 LED - Spider-Farmer Optic 1 54W 3500k - opticledgrowlights Optic 1 XL 100W 3500k – opticledgrowlights Optic 1 XL 100W 3500k (Dimming) – opticledgrowlights Cana COCO - Coco Brick Perlite - Sta-Green Vermiculite – Sta-Green Filtered Water – Everest Machine Botanicare Cal-MAG Plus General Hydroponic – Flora Series General Hydroponic – pH Up, pH Down Great White - Root Magic Mycorrhizae+ Real Growers – Recharge Fish Head Farms – Fish Shit Brer Rabbit – Blackstrap Molasses

I got the 3 starting seeds from an exchange with a redditor who accidentally pollinated his batch with a male, meaning I didn't start with the best genetic which in the end, probably induced the hermie. It turned out good overall but it did fuck up a lot of things. I never thought 4 weeks of veg would be too much. They got tall in no time and almost tripled in height during the flowering stretch. Light burn had a significant impact on the trichome production of certain buds. The mold is on me. I should have controlled the conditions better. I had to throw away 10-15g because of it. And really nothing guarantees I removed it all. Plant B produced, in the books, a lot less than her sisters and that can be explained by 3 factors: -Mold attacked it more than the others; -I probably switched 1 or maybe 5 (who knows...) whole branches when they fell from the rope during the drying; -I left a lot of tiny popcorn buds that got separated in the ''to be extracted'' jar. The 3 plants were pretty similar in looks but gave rather different seeds. A, the hermie, gave a lot of seeds in general but almost half of them were immature or wonky and got discarded; B, a female, gave a lot of good ones but also quite a few bad ones. The have nice stripes giving them a tiger look; C, another female, still hasn't been plucked of all it's seeds but the ones I already collected are really big, round and mat. Plant A pollinated itself. Plant B got helped by me but C didn't received help from me nor the wind and still got hit, a lot less, but still. I'll never underestimate the propagation power of pollen again. FINAL HARVEST: PLANT A: - 91g - 281 viable seeds PLANT B: - 28g - 297 viable seeds PLANT C: - 89g - 149 viable seeds 167 mixed seeds from ABC EXTRACT MATERIAL: - 9g TOTAL: 217g / 894 seeds Height by weeks: 1 - 7 cm 2 - 20 cm 3 - 32 cm 4 - 49 cm Switch 5 - 81 cm 6 - 107 cm 7 - 120 cm 8 - 124 cm 9 - 125 cm 10 - 126 cm 11 - 126 cm 12 - 128 cm 13 - 128 cm Nice experience overall. A lot of thing could have gone better but the fact is I got a bunch of seeds for future pheno hunting and God knows I'll need to do some to straighten the genetic. See you all then!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

I'm clearly going to have some tucking and training ahead of me again this week...For anyone who wonders if heavy defoliation is detrimental - last week these plants were almost totally stripped and were ALL pulled back under the screen - and I'd said that you'd never know they were bare. Well, here we are a week later and we're pretty much doing the same thing again! I'm blindly hoping to avoid tucking much more. The screen is full and the girls are powerlifting it off the floor - we'll have to get some weights on to keep the canopy level, but what do we really expect from Bruce Banner?? I've also got my fingers crossed that we can manage the rest of the stretch with defoliation, though that's probably unlikely and we'll be working a very full scrog and tent for the next few weeks (based on some diaries it's about 4 weeks of stretch before they settle into a solid budset - I wish I had read that before vegging the screen to 80% capacity!