I started the week by flushing the plants. It was time. The oldest two plants have two weeks max. The run off started at 1900ppm and went down to 400ppm. By that time I had pumped about 60 litres through the four of them. The tap water is already 300ppm, so this is a good level I think. The leaves are responding. I can see the nutrients being drawn out of them. They are withdrawing. I am also looking at trichomes daily now. The mature plants are 1/3 clear, 2/3 cloudy, with a few amber. The younger plants are 2/3 clear, 1/3 cloudy, with no amber. I couldn’t wait any longer. I cropped and chopped 3 buds from under the canopy and dried them slowly on a metal plate over boiling water. It took 1/2 hour, but it produced a nicely dried pile of smelly weed. If I lost any potency in the process we didn’t notice. We got really really stoned. If this sample is any indication, I think it will easily be my best grow yet in terms of potency. Now I’m curious about the weight.

🇺🇸Grow Journal🇺🇸 – Mother Gorilla – Vegetative Stage – Week 4 The plant has recovered from last week’s defoliation, although I have noticed a slight decrease in its vigor. I believe this is normal, but it’s still progressing and growing. Environmental Conditions • Humidity: 70% • Temperature: ~78.8°F • pH Level: Still between 5.8 and 6.2; it had slightly increased, so I adjusted it back down to 5.8 • VPD: Still between 0.4 and 0.8, which is fine Most of the heavy work was done last week, so this week has been more about maintaining progress. I’m continuing to train the plant using the SCROG method, carefully tucking branches underneath the net whenever possible. I also did a light defoliation to maximize light penetration for the new developing branches that are forming new growth sites. I’m still waiting for my new nutrients, which should arrive this week. That means I’ll finally be able to switch to a proper nutrient solution. So far, I’ve only been using Formulex, which has worked fine, but I believe it’s now time to transition to proper vegetative nutrients. Regarding the roots, they still seem to be developing well (secondary rootlets). For now, everything is going smoothly. The plant has recovered from defoliation and, most importantly, from topping. I will continue training it carefully, and we’ll see how it evolves over the coming week. Looking forward to next week—we’ll keep a close eye on the progress. See you next week! Journal de Culture – Mother Gorilla – Croissance – Semaine 4 La plante a récupéré de la défoliation de la semaine dernière, même si je note quand même une diminution de sa vigueur. Je pense que c’est normal, mais elle continue d’évoluer et de pousser. Constantes environnementales • Humidité : 70% • Température : ~26°C • pH : Toujours entre 5.8 et 6.2 ; il avait un peu augmenté, donc je l’ai redescendu à 5.8 • VPD : Toujours entre 0.4 et 0.8, donc tout est bon Le gros du travail ayant été fait la semaine dernière, cette semaine est surtout consacrée au suivi et à l’entretien. Je continue tranquillement le palissage avec le SCROG, en replaçant les branches sous le filet lorsque c’est possible. J’ai aussi fait une légère défoliation pour maximiser la pénétration de la lumière sur les nouvelles petites branches qui forment de nouveaux sites de croissance. J’attends toujours mes nouveaux nutriments, qui devraient arriver cette semaine. Je vais enfin pouvoir passer sur une véritable solution nutritive. Jusqu’à présent, j’ai uniquement utilisé Formulex, ce qui convenait, mais je pense qu’il est maintenant temps de passer aux engrais de croissance. Concernant les racines, elles ont l’air de bien se développer (radicelles secondaires). Pour l’instant, tout suit son cours. La plante a bien récupéré de sa défoliation et surtout de son topping. Je vais poursuivre le palissage tranquillement et on va suivre son évolution jusqu’à la semaine prochaine. Hâte d’être à la semaine prochaine, on va suivre ça de près. 🤘😎

La planta le encantó la nueva luz y empezó estirarse demasiado tanto que para controlar su altura apliqué una poda apical la cual respondió muy bien y empezo a sacar 2 puntas principales

------------------------- | BLUEBERRY HARVEST | ------------------------- After two sunny days it is harvest time !! - Finally, 16 days of flush - At first thought, the trimming would be easier compared to my previous plants, but when removing more leaves, more buds appearing too hahaha so it took like 4 hours or more..It is not the most accuurate trimming but i had not much time that day, i removed all the leaves and branches after weighting it all.. Like i said on the last post, im harvesting having in mind my bud preferences (the later the better), weather, humidity and sunlight, so the first of the cloudy/rainy days she was chopped in the moring, attending the principle of thc regeneration at night.. Got to say that, i wasnt expecting such production (8 days until it was well dried), this plant started as the season saver because the Cheese started to flower in march, revegged etc.. and i have been rewarded with this BB, a true genetic portent !! If i could, i would keep her as mother forever ^^ The quality is awesome, she looks more like a WW talking about that coat of thc.. Flavour without proper cure, is deep earthy at first, transitioning to a fruity, sweet one, it is a pleasure to smoke, delicious sweet aftertaste.. Maybe, this is the last year with this awesome climate, because im planning to leave Spain.. Thank you for follow and check !!! I hope you liked the diary :) Song of the week : Radiohead - Codex Good Vibes GrowDiaries !! ---------------------------------------------------------------------------------------------------------------------------------------------- 000000000000

Start Woche 4 Blüte. Die ersten wirklich warmen Tage... Samstag leider über 30°C Im Zelt...🙄🙄 Nun ja, müssen die Damen leider durch, ne Klima kommt einfach nicht in Frage😏 Deshalb habe ich ja Purolyt getestet. Bisher schaun beide gut aus👍 So langsam sind die ersten Trichome sichtbar und sie fangen an leicht zu riechen. Die beiden bekommen morgen noch ihren Composttee, sonst passiert grade nicht viel. Hin und wieder werden ein paar Blätter entfernt. 🍀 Tag71 Beide haben jeweils 2l Composttee bekommen.

Off to a good start , remove humidity dome once she pushed through

Buenas compis 6 semana ya ... Esto se va acabando Afrontamos las ultimas etapas de este seguimiento , en el que comenzaremos a limpiar raíces , en días Ya parece que van acabando su ciclo , así que irán bien de tiempo .. Mil gracias Un saludo

She's still growing well this week. 8cm growth. It's her first week on 12/12. Changed to 6-12-36 powdered nutrients now.

Finally got my blue dream from seedsman. Winner of the contest seeds of knowledge Here it is finally growing this beautiful lady I gotta say this one was almost broken, it arrived very well but since it was hidden as requested when I open the box with a razor to take out the seeds Packs my bad accidentally cut a tiny side of the seed Shell which I noticed and place into germination and now she is here with us and we are very happy to welcome her to our family Day 1 Tuesday Place into germination Fully at dark until next morning from 8AM Started to get light since she is a photoperiod At the end of the Day went and hid it in a box very well so its dark Day 2 Same process took her out and start again sunny Day for her indoors of course Day 3 Thursday finally got her showing its cotyledons doing the same process :3 And im guessing it goes with the sun At the end of the Day got to give her enough darkness she is a feminized seeds which I have to make her flower after idk some time so I will try and see how much she can grow and get STRESS for yield and potency, thanks a lot for your support Happy Friday btw 👽🌱 **** DAY 5 My girl is a monster and needs to recover Taproot was coming out of peatmoss and I didnt even notice so had to transplant to a Bigger pot soon, since she is not an auto she is way too fast cuz taproot was out in the bottom so I had to take the net out of the Peat moss so roots van expand comfortably but accidentally broke the tiny edge while sliding with lil to no pressure and it sucks but it was stuck and it was either that or just plant it like that but well probably I should have left her be but anyways she is young I think she will develop more since she is in a better soil mix now than just Peat moss, wish me luck cuz I really hope she is better and gets to grow huge, we will see at the process right This is whats all about Lets do our best tho We will! Enjoy & happy growing everyone ☺️

What another great week!! The 5 Fastbud Wedding Cheesecake autos are looking great!! I started on veg feed this week on Monday , 1 tsp Rapid start , 1 tsp Calmag, 2 tsp micro , 3 tsp gro and 1 tsp of bloom, PH at 6.5 !! They are underneath my HLG Scorpion Rspec at 100%pwr , 49 inches to floor at Par 500-520 ! Loving it in their AC Infinity 3 gal pots ! Can’t wait to see these babies in their prime!! 😍😍 Y’all have a great amazing safe weekend and don’t forget Peace, Love, an Positive Vibes to you all beautiful ppl! Happy grows ✌️💚 Cheers

Another straightforward week. Increased pk 13/14 and they’re starting to fatten up nicely and smell good to. Bit annoying the left plant has a few branches that have stretched out as I’d of liked to have got the light down a bit closer to the ones below.

Gracias al equipo de Seedsman y XpertNutrients sin ellos esto no sería posible. 💐🍁 Bubba Cheescake: Bubba Cheesecake es un cruce de Bubba Kush anterior al 98 con Cheese Cake (Wedding Cake x Exodus Cheese). Se trata de un híbrido 70% índica con muy buenos rendimientos, alto en THC y bajo en CBD. Bubba Cheesecake prospera en interiores y exteriores. En interior se aconseja un corto periodo de crecimiento vegetativo debido a la cantidad de estiramiento que presentan las plantas. Las plantas pueden crecer hasta una altura de 250 a 350 cm. cuando se deja crecer naturalmente al aire libre. En interior, la floración dura entre 60 y 65 días, con rendimientos entre buenos y altos, de 400 a 550 gr/m2. Las plantas de exterior son capaces de producir entre 700 - 800 gr. peso seco. En las latitudes norteñas, en exterior, los productores pueden esperar cosechar a principios de octubre. Los cogollos son grandes y duros como piedras. Las plantas maduras muestran atractivos colores púrpuras y azules y brillan con una resina pegajosa que cubrirá las manos del cultivador si no tiene cuidado. El componente Wedding Cake agrega dulzura al sabor del regaliz y el aroma es terroso y musgoso. La producción de THC es muy alta con un nivel bajo de CBD. El efecto es intensamente narcótico, fuerte y duradero. 🌻🚀 Consigue aqui tus semillas: 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 2: Fantástica semana, estan creciendo de una forma espectacular gracias a la ayuda de @marshydro, @xpertnutrients y @trolmaster. Se puede apreciar un tercer nudo y se ven aparecer las primeras futuras ramas, espero que todo siga asi. Saldrá al exterior cuando amaine este temporal. Dia 16: Se planta en su lugar definitivo, ahora si comienza la aventura 💃💃💃. Potencia de la lámpara: 50%

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Week 7, day 44

My First diary. First time growing Fastberry bay Fastbuds. Hyped af. Hope for a big plant. Maybe you'd Like to follow this grow.

Another good foundation week for this Mimosa Cake Auto,,,showing good potential for a great canopy,,,which is what im